AKT1, Scene 1: The Cell Must Go On

Wed, 03/19/2014 - 13:30

Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis and cell growth. Akt1 acts as a transducer for growth factor receptors that modulate phosphatidylinositol 3-kinase (PI3K) activity. Akt is believed to be a factor in cancer as the tumor suppressor phosphatase and tensin homolog (PTEN) was found to antagonize both PI-3 kinase and Akt kinase activity. Akt’s major phosphorylation sites located at threonine 308 and serine 473 are required for its activation and three mammalian isoforms have been identified. Spanish researchers used the Akt antibody in their characterizations of transgenic mice with membrane-targeted PI3K to monitor morphological changes and tumorigenesis in mammary ducts1. The same research group also quantitativelyanalyzed transgenic mice cell lines constitutively overexpressing Akt - Akt antibody was used to examine correlations between activity and malignant phenotype2. Another application of the Akt antibody involved its use by Pommier’s group to monitor Akt signaling through liver X receptors (LXRs) in lipid rafts, and these studies yielded promising data for reduction of prostate tumor growth through downregulation of LXR3.

Western Blot: AKT1 Antibody Western Blot: AKT1 Antibody

Elamin, et al. used the Akt antibody to test the utility of the natural nontoxic compound curcumin as a type of Sonic Hedgehog (Shh)-targeted therapy in aggressive medulloblastomas4. They determined that curcumin suppressed cell proliferation, triggered cell cycle arrest, and had cytotoxic effects in a C-myc, N-myc, and cyclin D1-dependent manner. The Akt antibody allowed Wedels’s lab to test a combinatorial administration of the kinase inhibitor AEE788 with a histone deacetylase (HDAC) inhibitor in the treatment of prostate cancer5.

  1. 19047141
  2. 20174572
  3. 20190811
  4. 20025076
  5. 20954195

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