Ago2/eIF2C2 [p Tyr393] Antibody

Western Blot: Ago2/eIF2C2 [p Tyr393] Antibody [NBP3-23364] - Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2/eIF2C2 (NBP3-23228) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt, Ch, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ago2/eIF2C2 [p Tyr393] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-Ago2/eIF2C2 (Tyr-393) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 393 in human Ago2/eIF2C2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2/eIF2C2. The site is also conserved in Ago1 and Ago4, but not in Ago3. [UniProt# Q9UKV8]
• Modification: p Tyr393
• Specificity: This antibody was cross-absorbed to unphospho-Ago2/eIF2C2 (Tyr-393), before affinity purification using phospho-Ago2/eIF2C2 (Tyr-393) peptide. The antibody detects a 97 kDa protein corresponding to the apparent molecular mass of phosphorylated Ago2/eIF2C2 on SDS-PAGE immunoblots of human A431 cells that were treated with EGF. This reactivity is not observed after lambda phosphatase dephosphorylation.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGO2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Ago2/eIF2C2 [p Tyr393] Antibody

• AGO2argonaute 2
• argonaute2
• EC 3.1.26.n1
• EC 3.1.26.n2
• eIF2C 2
• eIF-2C 2
• Eukaryotic translation initiation factor 2C 2
• eukaryotic translation initiation factor 2C, 2
• hAgo2
• MGC3183
• PAZ Piwi domain protein
• PPD
• protein argonaute-2
• Protein slicer
• Q10

Background

Ago2 / eIF2C2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGO2