Ago2/eIF2C2 Antibody

Western Blot: Ago2/eIF2C2 Antibody [NBP3-23235] - Western blot analysis of mouse recombinant Ago2/eIF2C2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2/eIF2C2 (NBP3-23235) (lanes 1 & 2) and rabbit polyclonal anti-Ago2/eIF2C2 (Ser-387) phospho-specific antibody (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt, Ch, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ago2/eIF2C2 Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Ago2/eIF2C2 synthetic peptide (coupled to KLH) corresponding to amino acids from the central region of human Ago2/eIF2C2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2/eIF2C2. [UniProt# Q9UKV8]
• Specificity: The antibody detects a 97 kDa protein corresponding to the apparent molecular mass of Ago2/eIF2C2 on SDS-PAGE immunoblots of human A431 cells and mouse recombinant Ago2/eIF2C2 full length protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGO2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Ago2/eIF2C2 Antibody

• AGO2argonaute 2
• argonaute2
• EC 3.1.26.n1
• EC 3.1.26.n2
• eIF2C 2
• eIF-2C 2
• Eukaryotic translation initiation factor 2C 2
• eukaryotic translation initiation factor 2C, 2
• hAgo2
• MGC3183
• PAZ Piwi domain protein
• PPD
• protein argonaute-2
• Protein slicer
• Q10

Background

Ago2 / eIF2C2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGO2