AG-2/AGR2 Antibody

Western Blot: AG-2 Antibody [NBP1-05936] - Human stomach lysate.

Immunocytochemistry/Immunofluorescence: AG-2 Antibody [NBP1-05936] - AGR2 antibody was tested in MCF7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: AG-2 Antibody [NBP1-05936] - Detection of AGR2 in mouse kidney.

Immunohistochemistry: AG-2 Antibody [NBP1-05936] - Detection of AGR2 in mouse prostate.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

AG-2/AGR2 Antibody Summary

• Immunogen: Synthetic peptide made to an N-terminal portion of mouse AGR2 (within residues 1-50). [Swiss-Prot# O88312]
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:100-1:300
• Application Notes: This AGR2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band can be seen at ~19.9 kDa. In ICC/IF cytoplasmic staining is observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  AG-2/AGR2 Protein (NBP1-05936PEP)

Reactivity Notes

Human, mouse and bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AG-2/AGR2 Antibody

• AG2
• AG-2
• AG2member 17
• AGR2
• anterior gradient 2 homolog (Xenopus laevis)
• anterior gradient homolog 2 (Xenopus laevis)
• GOB-4
• hAG-2
• HPC8
• PDIA17
• secreted cement gland homolog

Background

Originally discovered in Xenopus laevis wherein it plays a role during early neural development, AGR2 (anterior gradient protein 2 homolog) is now known as a member of protein disulfide isomerase (PDI) family of ER-resident proteins which is expressed in a variety of normal as well as transformed epithelial tissues. AGR2 has been implicated in the folding of proteins as a chaperone protein. It interacts with LYPD3, DAG1 (alphaDAG1) and MUC2 (disulfide-linked), and is essential for MUC2 translation as well as secretion. AGR2 plays a key role in the production of mucus by intestinal cells and has also been suggested to be a proto-oncogene that may involve in cellular migration, differentiation and growth. Differential expression of AGR2 was first found in estrogen receptor (ER)-positive breast cancer cell lines and subsequently, elevated AGR2 levels have been seen in subsets of other cancers also wherein its upregulation enhances classical hallmarks of cancer such as metastasis, invasion, colony formation, and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AG-2/AGR2 Antibody (NBP1-05936)(1)

Publication using NBP1-05936

• Maurel M, Obacz J, Avril T et al. Control of anterior GRadient 2 (AGR2) dimerization links endoplasmic reticulum proteostasis to inflammation EMBO Mol Med Apr 30 2019 [PMID: 31040128] (IHC-P, Human)

Bioinformatics

• Gene Symbol: AGR2