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AG-2/AGR2 Antibody

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Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-05933] - Detection of AGR2 in mouse prostate.
Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-05933] - Detection of AGR2 in mouse prostate.
Immunohistochemistry: AG-2 Antibody [NBP1-05933] - Detection of AGR2 in mouse kidney.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications IHC, IHC-P, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-05933AF350
Alexa Fluor 405
NBP1-05933AF405
Alexa Fluor 488
NBP1-05933AF488
Alexa Fluor 532
NBP1-05933AF532
Alexa Fluor 594
NBP1-05933AF594
Alexa Fluor 647
NBP1-05933AF647
Alexa Fluor 700
NBP1-05933AF700
Alexa Fluor 750
NBP1-05933AF750
Biotin
NBP1-05933B
DyLight 405
NBP1-05933V
DyLight 488
NBP1-05933G
DyLight 550
NBP1-05933R
DyLight 594
NBP1-05933DL594
DyLight 650
NBP1-05933C
DyLight 680
NBP1-05933FR
DyLight 755
NBP1-05933IR
FITC
NBP1-05933F
HRP
NBP1-05933H
Janelia Fluor 549
NBP1-05933JF549
Janelia Fluor 646
NBP1-05933JF646
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AG-2/AGR2 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human AGR2 (within residues 50-100). [Swiss-Prot# O95994]
Localization
Secreted
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:100-1:300
Application Notes
This AGR2 antibody is useful for Immunohistochemistry paraffin embedded sections. It is not applicable for Immunocytochemistry/Immunofluorescence.
Control Peptide
AG-2/AGR2 Protein (NBP1-05933PEP)

Reactivity Notes

Human and mouse. Predicted to react with bovine based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AG-2/AGR2 Antibody

  • AG2
  • AG-2
  • AG2member 17
  • AGR2
  • anterior gradient 2 homolog (Xenopus laevis)
  • anterior gradient homolog 2 (Xenopus laevis)
  • GOB-4
  • hAG-2
  • HPC8
  • PDIA17
  • secreted cement gland homolog

Background

Originally discovered in Xenopus laevis wherein it plays a role during early neural development, AGR2 (anterior gradient protein 2 homolog) is now known as a member of protein disulfide isomerase (PDI) family of ER-resident proteins which is expressed in a variety of normal as well as transformed epithelial tissues. AGR2 has been implicated in the folding of proteins as a chaperone protein. It interacts with LYPD3, DAG1 (alphaDAG1) and MUC2 (disulfide-linked), and is essential for MUC2 translation as well as secretion. AGR2 plays a key role in the production of mucus by intestinal cells and has also been suggested to be a proto-oncogene that may involve in cellular migration, differentiation and growth. Differential expression of AGR2 was first found in estrogen receptor (ER)-positive breast cancer cell lines and subsequently, elevated AGR2 levels have been seen in subsets of other cancers also wherein its upregulation enhances classical hallmarks of cancer such as metastasis, invasion, colony formation, and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-05933AF350
Alexa Fluor 405 NBP1-05933AF405
Alexa Fluor 488 NBP1-05933AF488
Alexa Fluor 532 NBP1-05933AF532
Alexa Fluor 594 NBP1-05933AF594
Alexa Fluor 647 NBP1-05933AF647
Alexa Fluor 700 NBP1-05933AF700
Alexa Fluor 750 NBP1-05933AF750
Biotin NBP1-05933B
DyLight 405 NBP1-05933V
DyLight 488 NBP1-05933G
DyLight 550 NBP1-05933R
DyLight 594 NBP1-05933DL594
DyLight 650 NBP1-05933C
DyLight 680 NBP1-05933FR
DyLight 755 NBP1-05933IR
FITC NBP1-05933F
HRP NBP1-05933H
Janelia Fluor 549 NBP1-05933JF549
Janelia Fluor 646 NBP1-05933JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol AGR2
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