AG-2/AGR2 Antibody

Immunohistochemistry-Paraffin: AG-2 Antibody [NB110-17780] - Immunohistochemical staining with NB110-17780, anti- AG-2 antibody, in formalin-fixed, paraffin-embedded normal colon tissue at 1:100 dilution.

• Reactivity: Hu, Bv
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

AG-2/AGR2 Antibody Summary

• Immunogen: A synthetic peptide corresponding to an internal region of human AGR2 (within residues 50-100). [UniProt# O95994]
• Localization: Secreted
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
• Application Notes: This AGR2 antibody is useful for ELISA and Immunohistochemistry paraffin embedded sections. Use in Western blot reported in scientific literature (PMID: 22072305).

Reactivity Notes

Human. Immunogen sequence has 94% homology to bovine and 88% homology to mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AG-2/AGR2 Antibody

• AG2
• AG-2
• AG2member 17
• AGR2
• anterior gradient 2 homolog (Xenopus laevis)
• anterior gradient homolog 2 (Xenopus laevis)
• GOB-4
• hAG-2
• HPC8
• PDIA17
• secreted cement gland homolog

Background

Originally discovered in Xenopus laevis wherein it plays a role during early neural development, AGR2 (anterior gradient protein 2 homolog) is now known as a member of protein disulfide isomerase (PDI) family of ER-resident proteins which is expressed in a variety of normal as well as transformed epithelial tissues. AGR2 has been implicated in the folding of proteins as a chaperone protein. It interacts with LYPD3, DAG1 (alphaDAG1) and MUC2 (disulfide-linked), and is essential for MUC2 translation as well as secretion. AGR2 plays a key role in the production of mucus by intestinal cells and has also been suggested to be a proto-oncogene that may involve in cellular migration, differentiation and growth. Differential expression of AGR2 was first found in estrogen receptor (ER)-positive breast cancer cell lines and subsequently, elevated AGR2 levels have been seen in subsets of other cancers also wherein its upregulation enhances classical hallmarks of cancer such as metastasis, invasion, colony formation, and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AG-2/AGR2 Antibody (NB110-17780)(6)

Publications using NB110-17780

• Xiao C, Li M, Huang Q, Si-Tu J SPAG9 promotes prostate cancer proliferation and metastasis via MAPK signaling pathway Am J Transl Res [PMID: 31497238]
• Alavi M, Mah V, Maresh EL et al. High expression of AGR2 in lung cancer is predictive of poor survival. BMC Cancer 2015 [PMID: 26445321] (IHC)
• Mao L, Yang C, Wang J et al. SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion. J Transl Med 2013 May 4 [PMID: 23642278] (IHC, Human)
• Wayner EA, Quek SI, Ahmad R, Ho ME, Loprieno MA, Zhou Y, Ellis WJ, True LD, Liu AY. Development of an ELISA to detect the secreted prostate cancer biomarker AGR2 in voided urine. Prostate. 2011 Nov 9. [PMID: 22072305] (WB, Human)
• Maresh EL, Mah V, Alavi M et al. Differential expression of anterior gradient gene AGR2 in prostate cancer. BMC Cancer;10(1):680. 2010 Dec 13. [PMID: 21144054]
• Wang J, Qin R, Ma Y et al. Differential gene expression in normal esophagus and Barrett's esophagus. J Gastroenterol 2009 [PMID: 19468668] (Human)

Bioinformatics

• Gene Symbol: AGR2
• Entrez:
  • 10551(Human)
• Uniprot:
  • O95994(Human)