AF4 Antibody - BSA Free

Immunoprecipitation: AF4 Antibody [NBP1-28705] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF4 antibody NBP1-28728 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. AF4 was also immunoprecipitated by rabbit anti-AF4 antibody NBP1-28705, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

AF4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AF4 Antibody - BSA Free (NBP1-28705) is a polyclonal antibody validated for use in WB and IP. Anti-AF4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human proto-oncogene AF4 using the numbering given in entry NP_005926.1 (GeneID 4299).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AFF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AF4 Antibody - BSA Free

• AF-4
• AF4/FMR2 family member 1
• AF4/FMR2 family, member 1
• AF4myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 2
• ALL1-fused gene from chromosome 4 protein
• FEL
• MLLT2MGC134969
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 2
• PBM1
• pre-B-cell monocytic leukemia partner 1
• Protein AF-4
• Protein FEL
• Proto-oncogene AF4

Background

AF4 is a member of the AF4/LAF4/FMR2 (ALF) family of transcription cofactors. It has been identified as part of a translocation [t(4:11)(q21;q23)] found in patients with acute leukemia involving MLL/HRX. AF4 functions as a cofactor of transcriptional elongation and chromatin remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AF4 Antibody (NBP1-28705)(2)

Publications using NBP1-28705

• Wu F, Nie S, Yao Y Et al. Small-molecule inhibitor of AF9/ENL-DOT1L/AF4/AFF4 interactions suppresses malignant gene expression and tumor growth Theranostics 2021-07-13 [PMID: 34373735] (WB)
• Mak AB, Nixon AM, Moffat J et al. The mixed lineage leukemia (MLL) fusion-associated gene AF4 promotes CD133 transcription. Cancer Res 2012-04-01 [PMID: 22337994]

Bioinformatics

• Gene Symbol: AFF1
• Entrez:
  • 4299(Human)
• Uniprot:
  • NP_005926.1(Human)