AF10 Antibody (S07-2G2)

Western Blot: AF10 Antibody (S07-2G2) [NBP3-19560] - Western blot detection of AF10 in Hela, K562 cell lysates using NBP3-19560 (1:1000 diluted). Predicted band size: 113kDa. Observed band size: 140kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S07-2G2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

AF10 Antibody (S07-2G2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human AF10 (Uniprot # P55197)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MLLT10
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for AF10 Antibody (S07-2G2)

• AF10MGC75086
• ALL1-fused gene from chromosome 10 protein
• DKFZp686E10210
• myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 10
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 10
• protein AF-10
• type I AF10 protein

Background

AF10 is a putative transcription factor containing a leucine zipper motif. A t(10;11)(p12;q23) chromosomal translocation present in a proportion of acute myeloid leukaemias results in expression of an AF10 (carboxy terminus) MLL (amino terminus) fusion protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLLT10