BRPF1 Antibody

Images

 
Western Blot: BRPF1 Antibody [NBP2-15620] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [N1N2], N-term diluted at 1:500. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: BRPF1 Antibody [NBP2-15620] - Mouse testis. Peregrin stained by Peregrin antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: BRPF1 Antibody [NBP2-15620] - Western Blot analysis of BRPF1 expression in transfected 293T cell line by BRPF1 polyclonal antibody. A: Non-transfected lysate. B: BRPF1 transfected lysate. 7. 5% SDS PAGE ...read more
Knockout Validated: BRPF1 Antibody [NBP2-15620] - Wild-type (WT) and Peregrin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRPF1 antibody diluted at 1:500. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BRPF1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human BRPF1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (96%), Chicken (91%), Bovine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRPF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin -Assay dependent
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
137 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for BRPF1 Antibody

  • BR140
  • bromodomain and PHD finger containing, 1
  • Bromodomain and PHD finger-containing protein 1
  • BRPF1
  • peregrin
  • Protein Br140

Background

The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BRPF1
Uniprot