Adiponutrin/PNPLA3 Antibody

Western Blot: Adiponutrin/PNPLA3 Antibody [NBP1-00227] - Staining of Mouse Adrenal Gland lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Adiponutrin/PNPLA3 Antibody [NBP1-00227] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunohistochemistry-Paraffin: Adiponutrin/PNPLA3 Antibody [NBP1-00227] - Staining of paraffin embedded Human Kidney. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Adiponutrin/PNPLA3 Antibody Summary

• Description: This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2. Other Gene IDs: 116939 (mouse); 362972 (rat)
• Immunogen: Peptide with sequence EHDICPKVKSTN corresponding to internal region according to Uniprot Mouse NP_473429.2.
• Specificity: This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PNPLA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 8 ug/mL
  • Peptide ELISA 1:16000
  • Western Blot 0.01 - 0.03 ug/mL
• Control Peptide:

  Adiponutrin/PNPLA3 Protein (NBP1-00227PEP)

Reactivity Notes

Expected from sequence similarity: Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Adiponutrin/PNPLA3 Antibody

• Acylglycerol O-acyltransferase
• Adiponutrin
• ADPN
• ADPNdJ796I17.1
• C22orf20
• Calcium-independent phospholipase A2-epsilon
• chromosome 22 open reading frame 20
• EC 2.3.1.-
• EC 2.7.7.56
• EC 3.1.1.3
• EC 4.2.3.4
• FLJ22012
• iPLA(2)epsilon
• iPLA2-epsilon
• patatin-like phospholipase domain containing 3
• patatin-like phospholipase domain-containing protein 3
• PNPLA3

Background

The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adiponutrin/PNPLA3 Antibody (NBP1-00227)(1)

Publication using NBP1-00227

• Steinberg GR, Kemp BE, Watt MJ. Adipocyte triglyceride lipase expression in human obesity. Am J Physiol Endocrinol Metab 2007-10-01 [PMID: 17609260]

Bioinformatics

• Gene Symbol: PNPLA3
• Entrez:
  • 80339(Human)
  • 116939(Mouse)
  • 362972(Rat)
• Uniprot:
  • NP_473429.2(Mouse)