Adiponectin/Acrp30 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by ...read more
Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Adiponectin/Acrp30 at approximately 41 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Adiponectin/Acrp30 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30
Glu19-Asn244
Accession # Q15848
Specificity
Detects human Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross‑reactivity with recombinant mouse Adiponectin is observed and less than 15% cross-reactivity with recombinant rat Adiponectin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADIPOQ
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1065 in the following applications:

Publications
Read Publications using
AF1065 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Adiponectin/Acrp30 Antibody [Unconjugated]

  • ACDC
  • Acrp30
  • ACRP30ADPN
  • adipocyte, C1Q and collagen domain containing
  • Adiponectin
  • adiponectin, C1Q and collagen domain containing
  • AdipoQ
  • ADIPQTL1
  • ApM1
  • apM-1
  • APM1APM-1
  • C1q and collagen domain-containing protein
  • GBP28
  • GBP28apM1
  • Gelatin-binding protein

Background

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10-12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).
  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  9. Waki, H. et al. (2005) Endocrinology 146:790.
  10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  12. Wang, Y. et al. (2008) Biochem. J. 409:623.
  13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Adiponectin/Acrp30 Antibody (AF1065)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: IHC, IHC-P, Native SDS-PAGE, Neutralization, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(1)
Native SDS-PAGE
(1)
Neutralization
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(8)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF1065 Applications Species
B Hu, X Lv, H Chen, P Xue, B Gao, X Wang, G Zhen, JL Crane, D Pan, S Liu, S Ni, P Wu, W Su, X Liu, Z Ling, M Yang, R Deng, Y Li, L Wang, Y Zhang, M Wan, Z Shao, H Chen, W Yuan, X Cao Sensory nerves regulate mesenchymal stromal cell lineage commitment by tuning sympathetic tones J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32191640] (IHC, Mouse) IHC Mouse
FJ Ruiz-Ojeda, A Anguita-Ru, AI Rupérez, C Gomez-Llor, J Olza, R Vázquez-Co, M Gil-Campos, G Bueno, R Leis, R Cañete, LA Moreno, A Gil, CM Aguilera Effects of X-chromosome Tenomodulin Genetic Variants on Obesity in a Children's Cohort and Implications of the Gene in Adipocyte Metabolism Sci Rep, 2019;9(1):3979. 2019 [PMID: 30850679] (Western Blot, Human) Western Blot Human
K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity J Immunol Res, 2017;2017(0):6468097. 2017 [PMID: 28424792] (Western Blot, Human) Western Blot Human
FJ Ruiz-Ojeda, C Gomez-Llor, CM Aguilera, A Gil, AI Rupérez Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes PLoS ONE, 2016;11(3):e0152550. 2016 [PMID: 27023799] (Western Blot, Human) Western Blot Human
Beier E, Sheu T, Dang D, Holz J, Ubayawardena R, Babij P, Puzas J Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY. J Biol Chem, 2015;290(29):18216-26. 2015 [PMID: 25975268] (Western Blot, Mouse) Western Blot Mouse
von Eynatten M, Liu D, Hock C, Oikonomou D, Baumann M, Allolio B, Korosoglou G, Morcos M, Campean V, Amann K, Lutz J, Heemann U, Nawroth PP, Bierhaus A, Humpert PM Urinary adiponectin excretion: a novel marker for vascular damage in type 2 diabetes. Diabetes, 2009;58(9):2093-9. 2009 [PMID: 19509019] (Western Blot, Human) Western Blot Human
Miller M, Cho JY, Pham A, Ramsdell J, Broide DH Adiponectin and functional adiponectin receptor 1 are expressed by airway epithelial cells in chronic obstructive pulmonary disease. J. Immunol., 2009;182(1):684-91. 2009 [PMID: 19109202] (IHC-P, Human) IHC-P Human
Ebinuma H, Miida T, Yamauchi T, Hada Y, Hara K, Kubota N, Kadowaki T Improved ELISA for selective measurement of adiponectin multimers and identification of adiponectin in human cerebrospinal fluid. Clin. Chem., 2007;53(8):1541-4. 2007 [PMID: 17599956] (Native SDS-PAGE, Human) Native SDS-PAGE Human
Retnakaran R, Connelly PW, Maguire G, Sermer M, Zinman B, Hanley AJ Decreased high-molecular-weight adiponectin in gestational diabetes: implications for the pathophysiology of Type 2 diabetes. Diabet. Med., 2007;24(3):245-52. 2007 [PMID: 17305786] (Western Blot, Human) Western Blot Human
Iversen PO, Wiig H Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis. Clin. Cancer Res., 2005;11(19):6793-9. 2005 [PMID: 16203766] (Neutralization, Human) Neutralization Human

Review for Adiponectin/Acrp30 Antibody (AF1065) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1065:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous -
Immunohistochemistry-Paraffin Human 01/05/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 21935928
SpeciesHuman

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Anonymous -
01/05/2015
Application: Immunohistochemistry-Paraffin
Species: Human

Bioinformatics

Gene Symbol ADIPOQ
Uniprot