Adiponectin/Acrp30 Antibody (553530R) [Unconjugated]

Recombinant Human Adiponectin/Acrp30 protein was serially diluted 2-fold and captured by Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 553530R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Adiponectin/Acrp30 Antibody (553530R) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30
  Glu19-Asn244
  Accession # Q15848
• Specificity: Detects human Adiponectin/Acrp30 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Adiponectin/Acrp30 Antibody (553530R) [Unconjugated]

• ACDC
• Acrp30
• ACRP30ADPN
• adipocyte, C1Q and collagen domain containing
• Adiponectin
• adiponectin, C1Q and collagen domain containing
• AdipoQ
• ADIPQTL1
• ApM1
• apM-1
• APM1APM-1
• C1q and collagen domain-containing protein
• GBP28
• GBP28apM1
• Gelatin-binding protein

Background

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in non-metabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1‑3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin 13/T-Cadherin, as well as with several growth factors (4‑7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8, 9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10‑12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
9. Waki, H. et al. (2005) Endocrinology 146:790.
10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
12. Wang, Y. et al. (2008) Biochem. J. 409:623.
13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q15848()