| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70101 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87406-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59414-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89878-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99767-50ul | |||
![Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Western-Blot-NBP2-03107-img0012.jpg "Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human lymphoma tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0010.jpg)
![Flow Cytometry: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Adenylosuccinate Lyase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Flow-Cytometry-NBP2-03107-img0001.jpg)
![Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adenylosuccinate Lyase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adenylosuccinate Lyase.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Western-Blot-NBP2-03107-img0002.jpg)
![Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Adenylosuccinate Lyase monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Western-Blot-NBP2-03107-img0011.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0003.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0005.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0007.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human Kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0008.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human liver tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0009.jpg)
![Adenylosuccinate Lyase Antibody (OTI2D10)[NBP2-03107]Simple Western� analysis of endogenous protein RNH1 from HELA lysates (0.5 mg/mL) using RNH1 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-03107_mouse-adenylosuccinate-lyase-mab-oti2d10-simple-western-131120251236350.jpg%253fstore=america&image-type=image)
![Western Blot: Adenylosuccinate Lyase Antibody [NBP1-87406] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADSL antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Western-Blot-NBP1-87406-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Adenylosuccinate Lyase Antibody [NBP1-87406] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADSL antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87406-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0014.jpg)
![Western Blot: Adenylosuccinate Lyase Antibody [NBP1-87406] - Analysis in human cell line HL-60.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Western-Blot-NBP1-87406-img0005.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human cerebral cortex shows no positivity in neuronal cells.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0010.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0011.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0012.jpg)
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-87406-img0013.jpg)
![Western Blot: Adenylosuccinate Lyase Antibody (16C10) [NBP2-59414] - Lane 1: Recombinant Protein, Lane 2: HeLa cell lysates, Lane 3: 293T cell lysates Lane 4: Jurkat cell lysates, Lane 5: HepG2 cell lysates Lane 6: A549 cell lysates Lane 7: MCF7 cell lysates](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-16C10-Western-Blot-NBP2-59414-img0001.jpg "Western Blot: Adenylosuccinate Lyase Antibody (16C10) [NBP2-59414] - Lane 1: Recombinant Protein, Lane 2: HeLa cell lysates, Lane 3: 293T cell lysates Lane 4: Jurkat cell lysates, Lane 5: HepG2 cell lysates Lane 6: A549 cell lysates Lane 7: MCF7 cell lysates")
![Immunocytochemistry/Immunofluorescence: Adenylosuccinate Lyase Antibody (16C10) [NBP2-59414] - Analysis of ADSL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ADSL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-16C10-Immunocytochemistry-Immunofluorescence-NBP2-59414-img0002.jpg)
![Flow Cytometry: Adenylosuccinate Lyase Antibody (16C10) [NBP2-59414] - Analysis of ADSL in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-16C10-Flow-Cytometry-NBP2-59414-img0003.jpg)
![Western Blot: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-007-Western-Blot-NBP2-89878-img0001.jpg "Western Blot: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:55 kDa")
![Immunoprecipitation: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-ADSL rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADSL rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-007-Immunoprecipitation-NBP2-89878-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Staining of ADSL in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89878 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89878_rabbit-monoclonal-adenylosuccinate-lyase-antibody-007-235202514492358.png)
![Western Blot: Adenylosuccinate Lyase Antibody [NBP2-15296] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE ASL antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Western-Blot-NBP2-15296-img0002.jpg "Western Blot: Adenylosuccinate Lyase Antibody [NBP2-15296] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE ASL antibody, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP2-15296] - Paraffin-embedded mouse fore brain. ADSL antibody [N3C3] dilution: 1:500.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP2-15296-img0003.jpg)
![Immunoprecipitation:Adenylosuccinate Lyase AntibodyNBP3-29821] -Detection of human Adenylosuccinate Lyase/ADSL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Adenylosuccinate Lyase/ADSL antibody NBP3-29821 (lot NBP3-29821-1) used for IP at 6 µg per reaction. Adenylosuccinate Lyase/ADSL was also immunoprecipitated by rabbit anti-Adenylosuccinate Lyase/ADSL antibody BL18708. For blotting immunoprecipitated Adenylosuccinate Lyase/ADSL, NBP3-29821 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29821_rabbit-adenylosuccinate-lyase-pab-3012202417141114.jpg "Immunoprecipitation:Adenylosuccinate Lyase AntibodyNBP3-29821] -Detection of human Adenylosuccinate Lyase/ADSL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Adenylosuccinate Lyase/ADSL antibody NBP3-29821 (lot NBP3-29821-1) used for IP at 6 µg per reaction. Adenylosuccinate Lyase/ADSL was also immunoprecipitated by rabbit anti-Adenylosuccinate Lyase/ADSL antibody BL18708. For blotting immunoprecipitated Adenylosuccinate Lyase/ADSL, NBP3-29821 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Adenylosuccinate Lyase Overexpression Lysate (Adult Normal) [NBL1-07363] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenylosuccinate Lyase.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07363-img0002.jpg "Western Blot: Adenylosuccinate Lyase Overexpression Lysate (Adult Normal) [NBL1-07363] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenylosuccinate Lyase.")
![Western Blot: Adenylosuccinate Lyase Antibody [NBP2-99767] - Anti-Adenylosuccinate Lyase rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-Western-Blot-NBP2-99767-img0002.jpg "Western Blot: Adenylosuccinate Lyase Antibody [NBP2-99767] - Anti-Adenylosuccinate Lyase rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa")
![Immunocytochemistry/ Immunofluorescence: Adenylosuccinate Lyase Antibody (05) [NBP3-26085] - Immunofluorescence staining of Adenylosuccinate Lyase in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-26085 (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Goat Anti-mouse IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-26085_mouse-adenylosuccinate-lyase-mab-05-azide-and-bsa-free-23520251628184.png "Immunocytochemistry/ Immunofluorescence: Adenylosuccinate Lyase Antibody (05) [NBP3-26085] - Immunofluorescence staining of Adenylosuccinate Lyase in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-26085 (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Goat Anti-mouse IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.")
![SDS-Page: Recombinant Human Adenylosuccinate Lyase Protein [NBP1-72514] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Adenylosuccinate-Lyase-Protein-SDS-Page-NBP1-72514-img0001.jpg "SDS-Page: Recombinant Human Adenylosuccinate Lyase Protein [NBP1-72514] - 15% SDS-PAGE (3ug)")