Reactivity | Hu, Mu, Rt, Ca, Ch, Fe, RbSpecies Glossary |
Applications | WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready |
Clone | HHF35 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | 1.0 mg/ml |
Immunogen | SDS extract of human myocardium. |
Localization | Cytoplasmic |
Marker | Muscle Cell Marker |
Specificity | This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | ACTA1 |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.) |
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | 10 mM PBS |
Preservative | No Preservative |
Concentration | 1.0 mg/ml |
Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NBP2-34634AF350 | |
Alexa Fluor 405 | NBP2-34634AF405 | |
Alexa Fluor 488 | NBP2-34634AF488 | |
Alexa Fluor 532 | NBP2-34634AF532 | |
Alexa Fluor 594 | NBP2-34634AF594 | |
Alexa Fluor 647 | NBP2-34634AF647 | |
Alexa Fluor 700 | NBP2-34634AF700 | |
Alexa Fluor 750 | NBP2-34634AF750 | |
Allophycocyanin | NBP2-34634APC | |
Allophycocyanin/Cy7 | NBP2-34634APCCY7 | |
Biotin | NBP2-34634B | |
DyLight 350 | NBP2-34634UV | |
DyLight 405 | NBP2-34634V | |
DyLight 488 | NBP2-34634G | |
DyLight 550 | NBP2-34634R | |
DyLight 594 | NBP2-34634DL594 | |
DyLight 650 | NBP2-34634C | |
DyLight 680 | NBP2-34634FR | |
DyLight 755 | NBP2-34634IR | |
FITC | NBP2-34634F | |
HRP | NBP2-34634H | |
Janelia Fluor 549 | NBP2-34634JF549 | |
Janelia Fluor 646 | NBP2-34634JF646 | |
PE | NBP2-34634PE | |
PE/Atto594 | NBP2-34634PEATT594 | |
PE/Cy5.5 | NBP2-34634PECY55 | |
PE/Cy7 | NBP2-34634PECY7 |
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