Actin (Muscle Specific) Antibody (HHF35) [Alexa Fluor® 750]


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Product Details

Reactivity Hu, Mu, Rt, Ca, Ch, Fe, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Alexa Fluor 750

Order Details

Actin (Muscle Specific) Antibody (HHF35) [Alexa Fluor® 750] Summary

SDS extract of human myocardium.
Muscle Cell Marker
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
IgG1 Kappa
Protein A purified
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  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein A purified


Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Actin (Muscle Specific) Antibody (HHF35) [Alexa Fluor® 750]

  • ACTA
  • ACTC
  • actin, alpha 2, smooth muscle, aorta
  • actin, alpha cardiac muscle 1
  • actin, alpha skeletal muscle
  • actin, aortic smooth muscle
  • alpha-cardiac actin
  • ASD5
  • ASMA
  • Cell growth-inhibiting gene 46 protein
  • CFTD
  • CFTD1
  • CMD1R
  • CMH11
  • MPFD
  • NEM1
  • NEM2
  • NEM3
  • nemaline myopathy type 3


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34634AF350
Alexa Fluor 405 NBP2-34634AF405
Alexa Fluor 488 NBP2-34634AF488
Alexa Fluor 532 NBP2-34634AF532
Alexa Fluor 594 NBP2-34634AF594
Alexa Fluor 647 NBP2-34634AF647
Alexa Fluor 700 NBP2-34634AF700
Alexa Fluor 750 NBP2-34634AF750
Allophycocyanin NBP2-34634APC
Allophycocyanin/Cy7 NBP2-34634APCCY7
Biotin NBP2-34634B
DyLight 350 NBP2-34634UV
DyLight 405 NBP2-34634V
DyLight 488 NBP2-34634G
DyLight 550 NBP2-34634R
DyLight 594 NBP2-34634DL594
DyLight 650 NBP2-34634C
DyLight 680 NBP2-34634FR
DyLight 755 NBP2-34634IR
FITC NBP2-34634F
HRP NBP2-34634H
Janelia Fluor 549 NBP2-34634JF549
Janelia Fluor 646 NBP2-34634JF646
PE NBP2-34634PE
PE/Atto594 NBP2-34634PEATT594
PE/Cy5.5 NBP2-34634PECY55
PE/Cy7 NBP2-34634PECY7

Additional Actin (Muscle Specific) Products

Array NBP2-34634AF750

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Gene Symbol ACTA1