Actin (Muscle Specific) Antibody (HHF35)

Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed paraffin-embedded human Rhabdomyosarcoma stained with muscle specific actin monoclonal antibody (HHF35)

Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Skeletal muscle tissue lysate using pan Actin Antibody (HHF35).

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed, paraffin-embedded human leiomyosarcoma (10X) stained with muscle specific actin MAb (HHF35)

Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Simple Western lane view shows a specific band for pan Actin in 0.2 mg/ml of Muscle (Sk) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Electropherogram image of the corresponding Simple Western lane. pan Actin antibody was used at 10 ug/ml dilution of Muscle (Sk) lysates(s) respectively.

• Reactivity: Hu, Mu, Rt, Ca, Ch, Fe, Rb
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: HHF35
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Actin (Muscle Specific) Antibody (HHF35) Summary

• Immunogen: SDS extract of human myocardium.
• Localization: Cytoplasmic
• Marker: Muscle Cell Marker
• Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1.0ug/ml
  • Simple Western 10 ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
• Application Notes: Human Chromosome Location: 1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
  Immunohistochemistry (No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Actin (Muscle Specific) Antibody (HHF35)

• 1a4 sma
• 1a4 smooth muscle actin
• ACTA
• ACTC
• actin, alpha 2, smooth muscle, aorta
• actin, alpha cardiac muscle 1
• actin, alpha skeletal muscle
• actin, aortic smooth muscle
• ACTSA
• alpha sma 1a4
• alpha-cardiac actin
• anti-alpha SMA
• ASD5
• ASMA
• Cell growth-inhibiting gene 46 protein
• CFTD
• CFTD1
• CFTDM
• CMD1R
• CMH11
• MPFD
• NEM1
• NEM2
• NEM3
• nemaline myopathy type 3
• sma 1a4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTA1
• Uniprot:
  • P68032()
  • P68133(Human)
  • P62736(Human)