Actin (Muscle Specific) Antibody (HHF35)

Images

 
Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed paraffin-embedded human Rhabdomyosarcoma stained with muscle specific actin monoclonal antibody (HHF35)
Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Skeletal muscle tissue lysate using pan Actin Antibody (HHF35).
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed, paraffin-embedded human leiomyosarcoma (10X) stained with muscle specific actin MAb (HHF35)
Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Simple Western lane view shows a specific band for pan Actin in 0.2 mg/ml of Muscle (Sk) lysate(s). This experiment was performed under reducing ...read more
Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Electropherogram image of the corresponding Simple Western lane. pan Actin antibody was used at 10 ug/ml dilution of Muscle (Sk) lysates(s) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Fe, RbSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
HHF35
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Actin (Muscle Specific) Antibody (HHF35) Summary

Immunogen
SDS extract of human myocardium.
Localization
Cytoplasmic
Marker
Muscle Cell Marker
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0ug/ml
  • Simple Western 10 ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
Application Notes
Human Chromosome Location: 1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
Immunohistochemistry (No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Actin (Muscle Specific) Antibody (HHF35)

  • 1a4 sma
  • 1a4 smooth muscle actin
  • ACTA
  • ACTC
  • actin, alpha 2, smooth muscle, aorta
  • actin, alpha cardiac muscle 1
  • actin, alpha skeletal muscle
  • actin, aortic smooth muscle
  • ACTSA
  • alpha sma 1a4
  • alpha-cardiac actin
  • anti-alpha SMA
  • ASD5
  • ASMA
  • Cell growth-inhibiting gene 46 protein
  • CFTD
  • CFTD1
  • CFTDM
  • CMD1R
  • CMH11
  • MPFD
  • NEM1
  • NEM2
  • NEM3
  • nemaline myopathy type 3
  • sma 1a4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Actin (Muscle Specific) Antibody (NBP2-34230) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34634AF350
Alexa Fluor 405 NBP2-34634AF405
Alexa Fluor 488 NBP2-34634AF488
Alexa Fluor 532 NBP2-34634AF532
Alexa Fluor 594 NBP2-34634AF594
Alexa Fluor 647 NBP2-34634AF647
Alexa Fluor 700 NBP2-34634AF700
Alexa Fluor 750 NBP2-34634AF750
Allophycocyanin NBP2-34634APC
Allophycocyanin/Cy7 NBP2-34634APCCY7
Biotin NBP2-34634B
DyLight 350 NBP2-34634UV
DyLight 405 NBP2-34634V
DyLight 488 NBP2-34634G
DyLight 550 NBP2-34634R
DyLight 594 NBP2-34634DL594
DyLight 650 NBP2-34634C
DyLight 680 NBP2-34634FR
DyLight 755 NBP2-34634IR
FITC NBP2-34634F
HRP NBP2-34634H
Janelia Fluor 549 NBP2-34634JF549
Janelia Fluor 646 NBP2-34634JF646
PE NBP2-34634PE
PE/Atto594 NBP2-34634PEATT594
PE/Cy5.5 NBP2-34634PECY55
PE/Cy7 NBP2-34634PECY7

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Bioinformatics

Gene Symbol ACTA1
Uniprot