Actin (Muscle Specific) Antibody (HHF35) [DyLight 755]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Fe, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
HHF35
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

Actin (Muscle Specific) Antibody (HHF35) [DyLight 755] Summary

Immunogen
SDS extract of human myocardium.
Localization
Cytoplasmic
Marker
Muscle Cell Marker
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Actin (Muscle Specific) Antibody (HHF35) [DyLight 755]

  • ACTA
  • ACTC
  • actin, alpha 2, smooth muscle, aorta
  • actin, alpha cardiac muscle 1
  • actin, alpha skeletal muscle
  • actin, aortic smooth muscle
  • ACTSA
  • alpha-cardiac actin
  • ASD5
  • ASMA
  • Cell growth-inhibiting gene 46 protein
  • CFTD
  • CFTD1
  • CFTDM
  • CMD1R
  • CMH11
  • MPFD
  • NEM1
  • NEM2
  • NEM3
  • nemaline myopathy type 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for pan Actin Antibody (NBP2-34634IR) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34634AF350
Alexa Fluor 405 NBP2-34634AF405
Alexa Fluor 488 NBP2-34634AF488
Alexa Fluor 532 NBP2-34634AF532
Alexa Fluor 594 NBP2-34634AF594
Alexa Fluor 647 NBP2-34634AF647
Alexa Fluor 700 NBP2-34634AF700
Alexa Fluor 750 NBP2-34634AF750
Allophycocyanin NBP2-34634APC
Allophycocyanin/Cy7 NBP2-34634APCCY7
Biotin NBP2-34634B
DyLight 350 NBP2-34634UV
DyLight 405 NBP2-34634V
DyLight 488 NBP2-34634G
DyLight 550 NBP2-34634R
DyLight 594 NBP2-34634DL594
DyLight 650 NBP2-34634C
DyLight 680 NBP2-34634FR
DyLight 755 NBP2-34634IR
FITC NBP2-34634F
HRP NBP2-34634H
Janelia Fluor 549 NBP2-34634JF549
Janelia Fluor 646 NBP2-34634JF646
PE NBP2-34634PE
PE/Atto594 NBP2-34634PEATT594
PE/Cy5.5 NBP2-34634PECY55
PE/Cy7 NBP2-34634PECY7

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Bioinformatics Tool for pan Actin Antibody (NBP2-34634IR)

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Calculators

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Bioinformatics

Gene Symbol ACTA1
Uniprot