Actin (Muscle Specific) Antibody (HHF35) [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt, Ca, Ch, Fe, Rb
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: HHF35
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Actin (Muscle Specific) Antibody (HHF35) [Alexa Fluor® 350] Summary

• Immunogen: SDS extract of human myocardium.
• Localization: Cytoplasmic
• Marker: Muscle Cell Marker
• Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Actin (Muscle Specific) Antibody (HHF35) [Alexa Fluor® 350]

• ACTA
• ACTC
• actin, alpha 2, smooth muscle, aorta
• actin, alpha cardiac muscle 1
• actin, alpha skeletal muscle
• actin, aortic smooth muscle
• ACTSA
• alpha-cardiac actin
• ASD5
• ASMA
• Cell growth-inhibiting gene 46 protein
• CFTD
• CFTD1
• CFTDM
• CMD1R
• CMH11
• MPFD
• NEM1
• NEM2
• NEM3
• nemaline myopathy type 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTA1
• Uniprot:
  • P68032()
  • P68133(Human)
  • P62736(Human)