ACSL5 Antibody (CL0275) - Azide and BSA Free

Analysis in human duodenum and tonsil tissues using NBP3-44076 antibody. Corresponding ACSL5 RNA-seq data are presented for the same tissues.

Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: ACSL5 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Staining of human endometrium shows strong membranous and cytoplasmic positivity in glandular cells.

Staining of human colon shows strong membranous positivity in glandular cells.

Staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0275
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ACSL5 Antibody (CL0275) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ACSL5 Antibody (CL0275) - Azide and BSA Free (NBP2-52899) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9ULC5, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACSL5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ACSL5 Antibody (CL0275) - Azide and BSA Free

• ACS2
• ACS5FACL5 for fatty acid coenzyme A ligase 5
• acyl-CoA synthetase long-chain family member 5
• EC 6.2.1
• EC 6.2.1.3
• FACL5fatty acid coenzyme A ligase 5
• fatty-acid-Coenzyme A ligase, long-chain 5
• LACS 5
• Long-chain acyl-CoA synthetase 5
• long-chain fatty acid coenzyme A ligase 5
• long-chain-fatty-acid--CoA ligase 5

Background

Long-chain-fatty-acid--CoA ligase 5, or ACSL5, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in both synthesis of cellular lipids and degradation via beta-oxidation. Like other isozymes of this family, ALCS5 converts free long-chain fatty acids into fatty acyl-CoA esters. Specifically, ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage.

This isozyme is highly expressed in uterus and spleen, and in trace amounts are found in normal brain. It also has significantly increased expression in malignant gliomas, where the protein functions in mediating fatty acid-induced glioma cell growth. Therefore, ALCS5 is thought to have a key role in the survival of glioma cells and may be a potential target for novel cancer treatments. Three transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACSL5