| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31995-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44076 | |||
| BSA Free | NBP2-52899-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35416-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97725-50ul | |||
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - ACSL5 monoclonal antibody (M01), clone 5H8 Analysis of ACSL5 expression in HepG2.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Western-Blot-H00051703-M01-img0012.jpg "Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - ACSL5 monoclonal antibody (M01), clone 5H8 Analysis of ACSL5 expression in HepG2.")
![Immunohistochemistry-Paraffin: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of monoclonal antibody to ACSL5 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Immunohistochemistry-Paraffin-H00051703-M01-img0009.jpg)
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of ACSL5 expression in transfected 293T cell line by ACSL5 monoclonal antibody (M01), clone 5H8.Lane 1: ACSL5 transfected lysate(82.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Western-Blot-H00051703-M01-img0013.jpg)
![Immunoprecipitation: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of ACSL5 transfected lysate using anti-ACSL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACSL5 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Immunoprecipitation-H00051703-M01-img0010.jpg)
![Sandwich ELISA: ACSL5 Antibody (5H8) [H00051703-M01] - Detection limit for recombinant GST tagged ACSL5 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Sandwich-ELISA-H00051703-M01-img0011.jpg)
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Representative western blotting (n = 3) for U87Empty, U87WT, and U87Mut cell protein extracts. The membranes were blotted with ACSL5 (H00051703-M01). The relative quantification of the bands is shown under the line. Blot was stripped and reprobed with anti-beta actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35574033/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/h00051703-m01_mouse-monoclonal-acsl5-antibody-5h8-25520231539511.jpg)
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Effects of IGF1R localization over cell metabolism. (A) U87Empty, U87WT, & U87Mut cells were cultured 24 h in serum-free medium or with 50-nM IGF1 stimulation (IGF1). To test the specificity of the response, pre-incubation (1 h) with 0.5 μM OSI906 was also performed (IGF1+ OSI). (A, B) GLUT1 mRNA expression was calculated by rqPCR by the relative quantitation method. (A) Values are presented as fold change compared to control conditions (serum-free). (B) IGF1 stimuli comparison between cell lines. The results are presented as fold change due to IGF1 stimulation over basal conditions (serum-free). Values are expressed as mean ± SD of three independent experiments performed in triplicates (*p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001, ANOVA, Tukey’s post-test). (C) LDH enzyme activity was measured & normalized to DNA content (mg). The results are expressed as mean ± SD of three independent experiments (**p < 0.005, ANOVA, Tukey’s post-test). (D) Representative western blotting (n = 3) for U87Empty, U87WT, & U87Mut cell protein extracts. The membranes were blotted with anti-pPDC (line 1), anti-FAS (line 3), anti-ACSL5 (line 5), & anti-pS6K (line 7). Each blot was stripped & reprobed with anti-beta actin (lines 2, 4, & 6) or antitotal-S6K (line 8). The relative quantification of the bands is shown under each line. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35574033), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00051703-m01_mouse-monoclonal-acsl5-antibody-5h8-310202415482271.jpg)
![Western Blot: ACSL5 Antibody [NBP1-59645] - Detection of ACSL5 in rat lysate. Image provided by Dr. Eric Klett of UNC School of Medicine.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NBP1-59645-img0004.jpg "Western Blot: ACSL5 Antibody [NBP1-59645] - Detection of ACSL5 in rat lysate. Image provided by Dr. Eric Klett of UNC School of Medicine.")
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP1-59645] - IHC analysis of human skeletal muscle tissue. Antibody concentration of 4 - 8ug/mL.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP1-59645-img0003.jpg)
![Western Blot: ACSL5 Antibody [NBP1-59645] - Human placenta tissue lysate. Antibody at a concentration of 1 ug/mL.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NBP1-59645-img0001.jpg)
![Simple Western: ACSL5 Antibody [NBP1-59645] - Simple Western analysis of mouse liver lysate. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Simple-Western-NBP1-59645-img0007.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining in human small intestine and pancreas tissues using anti-ACSL5 antibody. Corresponding ACSL5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP2-31995-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining in human small intestine and pancreas tissues using anti-ACSL5 antibody. Corresponding ACSL5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ACSL5 Antibody [NBP2-31995] - Analysis in control (vector only transfected HEK293T lysate) and ACSL5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NBP2-31995-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: ACSL5 Antibody [NBP2-31995] - Staining of human cell line U-251 MG shows localization to nucleoli & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31995-img0009.jpg)
![Western Blot: ACSL5 Antibody [NBP2-31995] - Analysis in human cell line CAPAN-2.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NBP2-31995-img0008.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP2-31995-img0010.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP2-31995-img0011.jpg)
![Western Blot: ACSL5 Antibody (CL0275) [NBP2-52899] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: ACSL5 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Western-Blot-NBP2-52899-img0006.jpg "Western Blot: ACSL5 Antibody (CL0275) [NBP2-52899] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: ACSL5 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)")
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human small intestine shows very strong cytoplasmic and membrane immunoreactivity in the glandular cells.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0005.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human colon (colorectal cancer) shows moderate membrane positivity in cancer cells.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0001.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human colon shows strong positivity in the intestinal glands.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0002.jpg)
![Immunohistochemistry: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human duodenum shows strong cytoplasmic and membrane immunoreactivity in the glandular cells.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-NBP2-52899-img0003.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human liver shows moderate cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ACSL5 Antibody [NB100-2918] - Staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NB100-2918-img0005.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NB100-2918] - Staining of human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NB100-2918-img0004.jpg)
![Immunohistochemistry: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31996_mouse-acsl5-mab-a9d3-r-298202414333655.jpg "Immunohistochemistry: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 76 kDa Observed band size: 65 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31996) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31996_mouse-acsl5-mab-a9d3-r-392024848509.jpg)
![Immunohistochemistry: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31995) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31995_mouse-acsl5-mab-a9d4-r-29820241492626.jpg "Immunohistochemistry: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31995) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31995) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31995_mouse-acsl5-mab-a9d4-r-3920248453515.jpg)
![Western Blot: ACSL5 Antibody [NBP3-35416] - Western blot analysis of various lysates using ACSL5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35416_rabbit-acsl5-pab-1012202411545513.jpg "Western Blot: ACSL5 Antibody [NBP3-35416] - Western blot analysis of various lysates using ACSL5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Western Blot: ACSL5 Overexpression Lysate (Adult Normal) [NBL1-07266] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ACSL5.](http://images.novusbio.com/fullsize/ACSL5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07266-img0002.jpg "Western Blot: ACSL5 Overexpression Lysate (Adult Normal) [NBL1-07266] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ACSL5.")
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-97725] - ACSL5 in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP2-97725-img0001.jpg "Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-97725] - ACSL5 in human liver with rabbit polyclonal antibody at 1:100 dilution.")