ACSL1 Antibody

Images

 
Western Blot: ACSL1 Antibody [NB110-99585] - Detection of ACSL in HepG2 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: ACSL1 Antibody [NB110-99585] - ACSL1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Simple Western: ACSL1 Antibody [NB110-99585] - Simple Western lane view shows a specific band for ACSL1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

ACSL1 Antibody Summary

Immunogen
Synthetic peptide made to an N-terminal portion of the human ACSL1 protein (within residues 1-100). [Swiss-Prot# P33121]
Localization
Single-pass type III membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACSL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:25 - 1:100
Application Notes
This ACSL1 antibody is useful for Western blot, where a band is seen approx. 78 kDa. In ICC/IF membrane staining was observed in HepG2 cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ACSL1 Peptide (NB110-99585PEP)
Publications
Read Publication using
NB110-99585 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, mouse, rat and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ACSL1 Antibody

  • ACS1LACS 2
  • Acyl-CoA synthetase 1
  • acyl-CoA synthetase long-chain family member 1
  • EC 6.2.1
  • FACL1EC 6.2.1.3
  • fatty-acid-Coenzyme A ligase, long-chain 1
  • LACS 1
  • LACSlong-chain 2
  • lignoceroyl-CoA synthase
  • Long-chain acyl-CoA synthetase 1
  • Long-chain acyl-CoA synthetase 2
  • Long-chain fatty acid-CoA ligase 2
  • long-chain fatty-acid-coenzyme A ligase 1
  • long-chain-fatty-acid--CoA ligase 1
  • Palmitoyl-CoA ligase 1
  • Palmitoyl-CoA ligase 2
  • paltimoyl-CoA ligase 1

Background

ACSL1 (long-chain-fatty-acid--CoA ligase 1), belongs to ATP-dependent AMP-binding enzyme family and is one of the 5 isoforms (ACSL - 1, -3, -4, -5 and -6) of long chain acyl CoA synthetases (ACSLs), the enzymes which plays a crucial role in regulating fatty acid metabolism by converting free fatty acids (FFAs) into fatty acyl CoA derivatives via a process called fatty acid activation, a modification required for any FFA to undergo further metabolism. ACSL1 is a single-pass type III membrane protein localized in mitochondrion outer membrane and membranes of peroxisomes, microsomes, ER of energy-metabolizing tissues such as fat, liver, and skeletal muscles. ACSL1 is a central enzyme in the intermediary metabolism due to its fundamental role in activation of long-chain fatty acids through the incorporation of CoA. These bioactive fatty acid metabolites are involved in many cellular biochemical processes, including protein transport, enzyme activation, and transcriptional control. Additionally, fatty acyl-CoAs are used as substrates in fatty acid beta-oxidation and the phospholipid biosynthesis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACSL1 Antibody (NB110-99585)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Product General Protocols

View specific protocols for ACSL1 Antibody (NB110-99585): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ACSL1 Antibody (NB110-99585) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-99585AF350
Alexa Fluor 405 NB110-99585AF405
Alexa Fluor 488 NB110-99585AF488
Alexa Fluor 532 NB110-99585AF532
Alexa Fluor 594 NB110-99585AF594
Alexa Fluor 647 NB110-99585AF647
Alexa Fluor 700 NB110-99585AF700
Alexa Fluor 750 NB110-99585AF750
Biotin NB110-99585B
DyLight 350 NB110-99585UV
DyLight 405 NB110-99585V
DyLight 488 NB110-99585G
DyLight 550 NB110-99585R
DyLight 594 NB110-99585DL594
DyLight 650 NB110-99585C
DyLight 680 NB110-99585FR
DyLight 755 NB110-99585IR
FITC NB110-99585F
HRP NB110-99585H
Janelia Fluor 549 NB110-99585JF549
Janelia Fluor 646 NB110-99585JF646

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Bioinformatics

Gene Symbol ACSL1