ACSL1 Antibody - BSA Free

Western Blot: ACSL1 Antibody [NB110-99585] - Detection of ACSL in HepG2 whole cell lysates.

Immunocytochemistry/Immunofluorescence: ACSL1 Antibody [NB110-99585] - ACSL1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Simple Western: ACSL1 Antibody [NB110-99585] - Simple Western lane view shows a specific band for ACSL1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ACSL1 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an N-terminal portion of the human ACSL1 protein (within residues 1-100). [Swiss-Prot# P33121]
• Localization: Single-pass type III membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACSL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:25 - 1:100
  • Simple Western 1:100
  • Western Blot 1 ug/ml
• Application Notes: This ACSL1 antibody is useful for Western blot, where a band is seen approx. 78 kDa. In ICC/IF membrane staining was observed in HepG2 cells.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ACSL1 Peptide (NB110-99585PEP)

Reactivity Notes

Human, mouse, rat and monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACSL1 Antibody - BSA Free

• ACS1LACS 2
• Acyl-CoA synthetase 1
• acyl-CoA synthetase long-chain family member 1
• EC 6.2.1
• FACL1EC 6.2.1.3
• fatty-acid-Coenzyme A ligase, long-chain 1
• LACS 1
• LACSlong-chain 2
• lignoceroyl-CoA synthase
• Long-chain acyl-CoA synthetase 1
• Long-chain acyl-CoA synthetase 2
• Long-chain fatty acid-CoA ligase 2
• long-chain fatty-acid-coenzyme A ligase 1
• long-chain-fatty-acid--CoA ligase 1
• Palmitoyl-CoA ligase 1
• Palmitoyl-CoA ligase 2
• paltimoyl-CoA ligase 1

Background

ACSL1 (long-chain-fatty-acid--CoA ligase 1), belongs to ATP-dependent AMP-binding enzyme family and is one of the 5 isoforms (ACSL - 1, -3, -4, -5 and -6) of long chain acyl CoA synthetases (ACSLs), the enzymes which plays a crucial role in regulating fatty acid metabolism by converting free fatty acids (FFAs) into fatty acyl CoA derivatives via a process called fatty acid activation, a modification required for any FFA to undergo further metabolism. ACSL1 is a single-pass type III membrane protein localized in mitochondrion outer membrane and membranes of peroxisomes, microsomes, ER of energy-metabolizing tissues such as fat, liver, and skeletal muscles. ACSL1 is a central enzyme in the intermediary metabolism due to its fundamental role in activation of long-chain fatty acids through the incorporation of CoA. These bioactive fatty acid metabolites are involved in many cellular biochemical processes, including protein transport, enzyme activation, and transcriptional control. Additionally, fatty acyl-CoAs are used as substrates in fatty acid beta-oxidation and the phospholipid biosynthesis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACSL1 Antibody (NB110-99585)(1)

Publication using NB110-99585

• Fernando H, Wiktorowicz JE, Soman KV et al. Liver proteomics in progressive alcoholic steatosis Toxicol Appl Pharmacol 2012-11-29 [PMID: 23200777] (WB, Rat)

Bioinformatics

• Gene Symbol: ACSL1