Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free

Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase

Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.

Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.

Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.

Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI3G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free (NBP2-02241) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACO2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 85.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free

• aconitase 2, mitochondrial
• Aconitase
• aconitate hydratase, mitochondrial
• ACONM
• Citrate hydro-lyase
• EC 4.2.1
• EC 4.2.1.3
• MGC20605
• MGC33908

Background

Aconitase 2 is encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACO2