Aconitase 1 Antibody

Western Blot: Aconitase 1 Antibody [NBP1-51965] - NBP1-51965 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Aconitase 1 Antibody Summary

• Immunogen: Peptide with sequence C-QVDFNRRADSLQKNQ corresponding to internal region according to NP_002188.1.
• Epitope: C-QVDFNRRADSLQKNQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Approx. 100 kDa band observed in human liver lysates (calculated MW of 98.4 kDa band according to NP_002188.1).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Aconitase 1 Antibody

• aconitase 1, soluble
• Aconitase
• aconitate hydratase
• Citrate hydro-lyase
• cytoplasmic aconitate hydratase
• EC 4.2.1
• EC 4.2.1.3
• Ferritin repressor protein
• IREB1IREBP1
• IRE-BP 1
• IREBP
• Iron regulatory protein 1
• iron-responsive element binding protein 1
• Iron-responsive element-binding protein 1
• IRP1ACONS

Background

Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aconitase 1 Antibody (NBP1-51965)(1)

Publication using NBP1-51965

• Wang W, Di X, D'Agostino RB et al. Excess capacity of the iron regulatory protein system. J Biol Chem 2007 Aug [PMID: 17604281]

Bioinformatics

• Gene Symbol: ACO1
• Entrez:
  • 48(Human)
  • 11428(Mouse)
  • 50655(Rat)
• Uniprot:
  • NP_002188.1(Human)