Acinus Antibody - BSA Free

Western Blot: Acinus Antibody [NBP1-76869] - K562 whole cell lysate with Acinus antibody (CP) at 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: Acinus Antibody - BSA Free [NBP1-76869] - Immunocytochemistry staining of K562 cells using Acinus antibody at 10 ug/mL.

Immunocytochemistry/ Immunofluorescence: Acinus Antibody - BSA Free [NBP1-76869] - Immunofluorescence of Acinus in Hela cells with Acinus antibody at 10 ug/mL.

Immunocytochemistry/ Immunofluorescence: Acinus Antibody - BSA Free [NBP1-76869] - Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 ug/ml.Green: Acinus Antibody Blue: DAPI staining

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Acinus Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Acinus Antibody - BSA Free (NBP1-76869) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to amino acids near the C-terminus of the cleaved active peptide p17, which are identical to those of mouse Acinus. The immunogen is located within amino acids 1050 - 1100 of Acinus. Amino Acid Squence: TRTALHGVKWPQSNPK
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACIN1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: An approximate 220 kDa band can be detected in Western Blot.
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Acinus Peptide (NBP1-76869PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Acinus Antibody - BSA Free

• Acinus
• ACN
• apoptotic chromatin condensation inducer 1
• apoptotic chromatin condensation inducer in the nucleus
• fSAP152
• functional spliceosome-associated protein 152
• KIAA0670DKFZp667N107

Background

Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE . A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACIN1
• Uniprot:
  • AAD56724(Human)