Acidic Calponin Antibody

Western Blot: Acidic Calponin Antibody [NBP1-51972] - NBP1-51972 (0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP1-51972] - (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Acidic Calponin Antibody Summary

• Immunogen: Peptide with sequence C-HGEYQDDYPRDYQYS corresponding to C-Terminus according to NP_001830.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.2 - 0.6 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in human duodenum, heart and kidney lysates (calculated MW of 36.4 kDa band according to NP_001830.1). Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Lung (Bronchus).
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Acidic Calponin Antibody

• calponin 3, acidic
• Calponin, acidic isoform
• calponin-3
• dJ639P13.2.2 (acidic calponin 3)

Background

This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acidic Calponin Antibody (NBP1-51972)(1)

Publication using NBP1-51972

• Vinatzer U, Gollinger M, Mullauer L et al. Mucosa-associated lymphoid tissue lymphoma: novel translocations including rearrangements of ODZ2, JMJD2C, and CNN3. Clinical Cancer Res 2008-10-15 [PMID: 18927281]

Bioinformatics

• Gene Symbol: CNN3
• Entrez:
  • 1266(Human)
• Uniprot:
  • Q15417(Human)