ACE-2 Antibody (535919) [Alexa Fluor® 647]

HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse anti-Human Alexa Fluor® 647-conjugated ACE-2 Monoclonal Antibody (Catalog # FAB9332R). Quadrant markers were set based on Mouse IgG2A Isotype Control (IC003R, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 535919
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

ACE-2 Antibody (535919) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ACE-2
  Gln18-Ser740
  Accession # Q9BYF1
• Specificity: Detects human ACE-2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Reconstitution Instructions: Reconstitution default not known

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody (535919) [Alexa Fluor® 647]

• ACE2
• ACE-2
• ACEH
• ACEHangiotensin I converting enzyme 2
• ACE-related carboxypeptidase
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• angiotensin-converting enzyme 2
• Angiotensin-converting enzyme homolog
• DKFZp434A014
• EC 3.4.17
• EC 3.4.17.23
• Metalloprotease MPROT15

Background

Angiogensin I Converting Enzyme-2 (ACE-2), also called ACEH (ACE homolog), is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function (1). ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.

1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
2. Crackower,  M.A. et al. (2002) Nature 417:822.
3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE-2 Antibody (FAB9332R)(10)

Publications using FAB9332R

• Suryawanshi, RK;Jaishankar, P;Correy, GJ;Rachman, MM;O'Leary, PC;Taha, TY;Zapatero-Belinchón, FJ;McCavitt-Malvido, M;Doruk, YU;Stevens, MGV;Diolati, ME;Jogalekar, MP;Richards, AL;Montano, M;Rosecrans, J;Matthay, M;Togo, T;Gonciarz, RL;Gopalkrishnan, S;Neitz, RJ;Krogan, NJ;Swaney, DL;Shoichet, BK;Ott, M;Renslo, AR;Ashworth, A;Fraser, JS; The Mac1 ADP-ribosylhydrolase is a Therapeutic Target for SARS-CoV-2 bioRxiv : the preprint server for biology 2024-08-09 [PMID: 39149230] (Flow Cytometry, Human)
• Park, C;Hwang, IY;Yan, SL;Vimonpatranon, S;Wei, D;Van Ryk, D;Girard, A;Cicala, C;Arthos, J;Kehrl, JH; Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage eLife 2024-03-20 [PMID: 38507462] (Flow Cytometry, Human)
• Hou, J;Wei, Y;Zou, J;Jaffery, R;Sun, L;Liang, S;Zheng, N;Guerrero, AM;Egan, NA;Bohat, R;Chen, S;Zheng, C;Mao, X;Yi, SS;Chen, K;McGrail, DJ;Sahni, N;Shi, PY;Chen, Y;Xie, X;Peng, W; Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection Nature communications 2024-01-02 [PMID: 38168026] (Flow Cytometry, Human)
• AD López-Muño, I Kosik, J Holly, JW Yewdell Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunity Science Advances, 2022-08-03;8(31):eabp9770. 2022-08-03 [PMID: 35921414] (Flow Cytometry, Human)
• L El-Shennaw, AD Hoffmann, NK Dashzeveg, KM McAndrews, PJ Mehl, D Cornish, Z Yu, VL Tokars, V Nicolaescu, A Tomatsidou, C Mao, CJ Felicelli, CF Tsai, C Ostiguin, Y Jia, L Li, K Furlong, J Wysocki, X Luo, CF Ruivo, D Batlle, TJ Hope, Y Shen, YK Chae, H Zhang, VS LeBleu, T Shi, S Swaminatha, Y Luo, D Missiakas, GC Randall, AR Demonbreun, MG Ison, R Kalluri, D Fang, H Liu Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2 Nature Communications, 2022-01-20;13(1):405. 2022-01-20 [PMID: 35058437] (Flow Cytometry, Human)
• GM Castiglion, L Zhou, Z Xu, Z Neiman, CF Hung, EJ Duh Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by�epistasis acting on ACE2 PloS Biology, 2021-12-21;19(12):e3001510. 2021-12-21 [PMID: 34932561] (Flow Cytometry, Human)
• AD López-Muño, I Kosik, J Holly, JW Yewdell Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity bioRxiv : the preprint server for biology, 2021-12-13;0(0):. 2021-12-13 [PMID: 34931195] (Flow Cyomtetry, Flow Cytometry, Hamster, Human, Primate - C. aethiops)
• J Yewdell, A Lopez-Muno, I Kosik, J Holly Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity Research square, 2021-12-13;0(0):. 2021-12-13 [PMID: 34931184] (Flow Cytometry, Chinese Hamster, Hamster - Mesocricetus auratus (Golden Hamster), Human)
• A Hudák, A Letoha, L Szilák, T Letoha Contribution of Syndecans to the Cellular Entry of SARS-CoV-2 International Journal of Molecular Sciences, 2021-05-19;22(10):. 2021-05-19 [PMID: 34069441] (Flow Cytometry, Human)
• R Liu, JL Americo, CA Cotter, PL Earl, N Erez, C Peng, B Moss MVA Vector Vaccines Inhibit SARS CoV-2 Replication in Upper and Lower Respiratory Tracts of Transgenic Mice and Prevent Lethal Disease bioRxiv : the preprint server for biology, 2021-01-01;0(0):. 2021-01-01 [PMID: 33442693] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • Q9BYF1()