ACE-2 Antibody (171606) [Unconjugated]

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Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB933) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
171606
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

ACE-2 Antibody (171606) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740 (predicted)
Accession # Q9BYF1
Specificity
Detects human ACE-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ACE-1 or rhNeprilysin is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ACE2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB933 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody (171606) [Unconjugated]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

Angiogensin I Converting Enzyme-2 (ACE-2), also called ACEH (ACE homolog), is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function (1). ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACE-2 Antibody (MAB933)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Functional Assay, IHC-P, Western Blot.


Filter By Application
Functional Assay
(1)
IHC-P
(1)
Western Blot
(4)
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Human
(5)
Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using MAB933 Applications Species
M Yamaguchi, S Hirai, T Sumi, Y Tanaka, M Tada, Y Nishii, T Hasegawa, H Uchida, G Yamada, A Watanabe, H Takahashi, Y Sakuma Angiotensin-converting enzyme 2 is a potential therapeutic target for EGFR-mutant lung adenocarcinoma Biochem. Biophys. Res. Commun., 2017;0(0):. 2017 [PMID: 28433633] (IHC-P, Human) IHC-P Human
Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol, 2014;88(2):1293-307. 2014 [PMID: 24227843] (Functional Assay, Human) Functional Assay Human
Iwata M, Silva Enciso JE, Greenberg BH Selective and specific regulation of ectodomain shedding of angiotensin-converting enzyme 2 by tumor necrosis factor alpha-converting enzyme. Am. J. Physiol., Cell Physiol., 2009;297(5):C1318-29. 2009 [PMID: 19759332] (Western Blot, Mouse) Western Blot Mouse
Lovren F, Pan Y, Quan A, Teoh H, Wang G, Shukla PC, Levitt KS, Oudit GY, Al-Omran M, Stewart DJ, Slutsky AS, Peterson MD, Backx PH, Penninger JM, Verma S Angiotensin converting enzyme-2 confers endothelial protection and attenuates atherosclerosis. Am. J. Physiol. Heart Circ. Physiol., 2008;295(4):H1377-84. 2008 [PMID: 18660448] (Western Blot, Human) Western Blot Human
Mizutani T, Fukushi S, Saijo M, Kurane I, Morikawa S JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells. Biochim. Biophys. Acta, 2005;1741(1):4-10. 2005 [PMID: 15916886] (Western Blot, Human) Western Blot Human
Hofmann H, Geier M, Marzi A, Krumbiegel M, Peipp M, Fey GH, Gramberg T, Pohlmann S Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor. Biochem. Biophys. Res. Commun., 2004;319(4):1216-21. 2004 [PMID: 15194496] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol ACE2
Uniprot