ABCB5 Antibody [Alexa Fluor® 647]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 647

ABCB5 Antibody [Alexa Fluor® 647] Summary

• Immunogen: A synthetic peptide made to an internal portion of the human ABCB5 protein (between residues 150-200) [UniProt Q2M3G0]
• Localization: Cell membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCB5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ABCB5 Antibody [Alexa Fluor® 647]

• ABCB5 P-gp
• ABCB5
• ABCB5alpha
• ABCB5beta
• ATP-binding cassette protein
• ATP-binding cassette sub-family B member 5
• ATP-binding cassette, sub-family B (MDR/TAP), member 5
• EC 3.6.3
• EC 3.6.3.44
• EST422562
• P-glycoprotein ABCB5

Background

ATP-Binding Cassette proteins comprise a superfamily of transporters with a wide variety of substrates. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. It has been identified as a novel drug transporter in human malignant melanoma. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for ABCB5 Antibody (NBP1-77687AF647) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 06/21/2016

Summary

• Application: Flow Cytometry
• Sample Tested: Primary mouse melanoma
• Species: Mouse
• Lot: A-3-0050916-AF647

Bioinformatics

• Gene Symbol: ABCB5