ABCA4 Antibody

Immunocytochemistry/Immunofluorescence: ABCA4 Antibody [NBP3-00524] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunocytochemistry/Immunofluorescence: ABCA4 Antibody [NBP3-00524] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Ca
• Applications: ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ABCA4 Antibody Summary

• Description: Novus Biologicals Goat ABCA4 Antibody (NBP3-00524) is a polyclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KQQTESHDLPLHPR corresponding to C-Terminus according to NP_000341.2.
• Epitope: C-KQQTESHDLPLHPR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABCA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
• Application Notes: Immunofluorescence: Strong expression of the protein seen in the Endoplasmic Reticulum of MCF7 and HeLa cells.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCA4 Antibody

• ABC10
• ABCRRMP
• ARMD2DKFZp781N1972
• ATP binding cassette transporter
• ATP-binding cassette sub-family A member 4
• ATP-binding cassette transporter, retinal-specific
• ATP-binding cassette, sub-family A (ABC1), member 4
• ATP-binding transporter, retina-specific
• CORD3
• EC 3.6.3
• FFMFLJ17534
• photoreceptor rim protein
• retinal-specific ATP-binding cassette transporter
• retina-specific ABC transporter
• RIM ABC transporter
• RIM protein
• RmP
• RP19
• Stargardt disease protein
• STGD
• STGD1

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is a retina-specific ABC transporter with N-retinylidene-PE as a substrate. It is expressed exclusively in retina photoreceptor cells, indicating the gene product mediates transport of an essental molecule across the photoreceptor cell membrane. Mutations in this gene are found in patients diagnosed with Stargardt disease, a form of juvenile-onset macular degeneration. Mutations in this gene are also associated with retinitis pigmentosa-19, cone-rod dystrophy type 3, early-onset severe retinal dystrophy, fundus flavimaculatus, and macular degeneration age-related 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABCA4