A20/TNFAIP3 Antibody (775912) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 775912
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

A20/TNFAIP3 Antibody (775912) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human A20
  Lys91-Leu263
  Accession # P21580
• Specificity: Detects human A20 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human A20/TNFAIP3 (aa 440-790) is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A20/TNFAIP3 Antibody (775912) [Alexa Fluor® 647]

• A20
• A20TNFA1P2
• EC 3.4.19.12
• EC 6.3.2.-
• MGC104522
• MGC138687
• OTU domain-containing protein 7C
• OTUD7C
• OTUD7CMGC138688
• Putative DNA-binding protein A20
• TNF alpha-induced protein 3
• TNFA1P2
• TNFAIP3
• tumor necrosis factor alpha-induced protein 3
• tumor necrosis factor inducible protein A20
• tumor necrosis factor, alpha-induced protein 3
• Zinc finger protein A20

Background

A20 (TNF alpha -induced protein 3) is a cytoplasmic zinc finger protein that inhibits NF kappa B activity and tumor necrosis factor-mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF-induced NF kappa B-dependent gene expression by interfering with RIP- or TRAF2-mediated transactivation signaling. A20 contains an N-terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor-interacting protein (RIP), thus mediating distinct regulatory effects in the down-regulation of NF kappa B signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for A20/TNFAIP3 Antibody (FAB7598R). (Showing 1 - 1 of 1 FAQ).

1. Following cleavage of A20/TNFAIP3, two full length fragments are released, one with 37 kDa and other one was approx. at 52 kDa. There are mentioning of 75Kda fragment and 37 KDa fragment in scientific papers but not 52 KDa. What is the significance of that specific band?
   • There is data available that explains 52 KDa band in A20/TNFAIP3. Using this antibody in Western Blot will result multiple bands. In LPS treatment, 52 KDa putative cleavage band was observed; however, the same band was not found in untreated THP1 cells.