5T4 Antibody

Western Blot: 5T4 Antibody [H00007162-B01P] - Analysis of TPBG expression in transfected 293T cell line by TPBG polyclonal antibody. Lane 1: TPBG transfected lysate(46.2 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated

5T4 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: TPBG (NP_006661.1, 1 a.a. - 420 a.a.) full-length human protein. MPGGCSRGPAAGDGRLRLARLALVLLGWVSSSSPTSSASSFSSSAPFLASAVSAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPAYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRAGAFEHLPSLRQLDLSHNPLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTSYVFLGIVLALIGAIFLLVLYLNRKGIKKWMHNIRDACRDHMEGYHYRYEINADPRLTNLSSNSDV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: TPBG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: It has been used for WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for 5T4 Antibody

• 5T4 oncofetal trophoblast glycoprotein
• 5T4 oncotrophoblast glycoprotein
• 5T4
• 5T4AG
• 5T4-AG
• 5T4M6P1,5T4 oncofetal antigen
• M6P1
• TPBG
• trophoblast glycoprotein

Background

The 5T4 gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is ass

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for 5T4 Antibody (H00007162-B01P). (Showing 1 - 1 of 1 FAQ).

1. What lysis buffer conditions are recommended. What positive controls for WB are recommended?
   • We distribute H00007162-B01P for Abnova, a company based in Taiwan, and are not involved in the development or testing of this product. I have attached their recommended protocol for western blot for you, which should contact all of the information that you need for your protocol. For a positive control, an over-expression lysate for 5T4 would work great (such as H00007162-T01 or NBL1-17210), as well as running a recombinant (or purified) 5T4 protein on a gel (like H00007162-P01). I hope this helps

Bioinformatics

• Gene Symbol: TPBG
• Uniprot:
  • NP_006661.1(Human)