53BP1 Antibody Pack

Western Blot: 53BP1 Antibody Pack [NB100-926] - Analysis of human 53BP1, using NB100-305. Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 foci in proliferating MO59K cells; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours. Images captured in a Kodak digital image system on a Leica fluorescent microscope.

Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Placenta, Villi 40X. Using NB100-304.

Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - Detection of 53bp1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml anti-53bp1 NB100-304 in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red).

Western Blot: 53BP1 Antibody Pack [NB100-926] - Detection of Human [deleted mouse] 53BP1 by Western Blot on Whole cell lysate from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel using NB100-304.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 foci in proliferating MEFs; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours. Images captured in a Kodak digital image system on a Leica fluorescent microscope.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC
• Host: Rabbit
• Concentration: Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

53BP1 Antibody Pack Summary

• Immunogen: See individual datasheets
• Specificity: These antiboides are specific for 53BP1.
• Host: Rabbit
• Gene: TP53BP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Application Notes: The antibodies included in this pack are useful for Western blot and immunocytochemistry. See individual datasheets for suggested working dilutions.

Reactivity Notes

Human and mouse. Other species have not been tested.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Concentration: Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
• Purity: Immunogen affinity purified

Notes

1 vial each of: NB100-304 (0.1ml) and NB100-305 (0.1ml).

Alternate Names for 53BP1 Antibody Pack

• 53BP1
• MGC138366
• p202
• p53-binding protein 1
• p53BP1,53BP1FLJ41424
• TP53BP1
• tumor protein 53-binding protein, 1
• tumor protein p53 binding protein 1
• tumor protein p53-binding protein, 1
• tumor suppressor p53-binding protein 1

Background

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Publications for 53BP1 Antibody Pack (NB100-926)(3)

Publications using NB100-926

• Wang Z, Yin H, Zhang Y et al. miR-214-mediated downregulation of RNF8 induces chromosomal instability in ovarian cancer cells. Cell Cycle 2014 [PMID: 25483088] (WB)
• Sin HS, Barski A, Zhang F et al. RNF8 regulates active epigenetic modifications and escape gene activation from inactive sex chromosomes in post-meiotic spermatids Genes Dev 2012 Dec 15 [PMID: 23249736] (IHC, ICC/IF, Mouse)
• Kang Y, Lee J-H, Hoan NN et al. Protein Phosphatase 5 Regulates the Function of 53BP1 after Neocarzinostatin-induced DNA Damage. J Biol Chem;284(15):9845-9853. 2009 [PMID: 19176521]

FAQs for 53BP1 Antibody Pack (NB100-926). (Showing 1 - 2 of 2 FAQs).

1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
   • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
   • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

Bioinformatics

• Gene Symbol: TP53BP1
• Entrez:
  • 7158(Human)
  • 27223(Mouse)
  • 296099(Rat)