53BP1 Antibody Pack

Images

 
Western Blot: 53BP1 Antibody Pack [NB100-926] - Analysis of human 53BP1, using NB100-305. Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 foci in proliferating MO59K cells; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours. ...read more
Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Placenta, Villi 40X. Using NB100-304.
Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - Detection of 53bp1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml anti-53bp1 NB100-304 in a 150 mcl reaction. Isotype control (black), ...read more
Western Blot: 53BP1 Antibody Pack [NB100-926] - Detection of Human [deleted mouse] 53BP1 by Western Blot on Whole cell lysate from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel using NB100-304.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 foci in proliferating MEFs; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours. Images ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Host
Rabbit
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Order Details

53BP1 Antibody Pack Summary

Immunogen
See individual datasheets
Specificity
These antiboides are specific for 53BP1.
Host
Rabbit
Gene
TP53BP1
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
The antibodies included in this pack are useful for Western blot and immunocytochemistry. See individual datasheets for suggested working dilutions.
Publications
Read Publications using
NB100-926 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Human and mouse. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative
0.09% Sodium Azide
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Purity
Immunogen affinity purified

Kit Components

Notes

1 vial each of: NB100-304 (0.1ml) and NB100-305 (0.1ml).

Alternate Names for 53BP1 Antibody Pack

  • 53BP1
  • MGC138366
  • p202
  • p53-binding protein 1
  • p53BP1,53BP1FLJ41424
  • TP53BP1
  • tumor protein 53-binding protein, 1
  • tumor protein p53 binding protein 1
  • tumor protein p53-binding protein, 1
  • tumor suppressor p53-binding protein 1

Background

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Publications for 53BP1 Antibody Pack (NB100-926)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 53BP1 Antibody Pack (NB100-926). (Showing 1 - 2 of 2 FAQs).

  1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
    • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
  2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
    • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

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Blogs on 53BP1.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Blocking 53BP1 Expression Lessens Tumor Development in BRCA1-Defective Mice
Our antibody database at Novus Biologicals provides research tools for the forefront of cancer research. Recently, a mouse study using 53BP1 and BRCA1 antibodies showed that deletion of 53BP1 greatly lessened the incidence of tumor development in mice...  Read full blog post.

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Bioinformatics

Gene Symbol TP53BP1
Entrez