5'-Nucleotidase/CD73 Antibody [Unconjugated]

Images

 
Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or isotype control antibody (Catalog # ...read more
5'‑Nucleotidase/CD73 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 10 µg/mL ...read more
5'‑Nucleotidase/CD73 was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4488) at 15 µg/mL ...read more
Mouse CD4+ splenocytes was stained with Sheep Anti-Mouse/Porcine 5'‑Nucleotidase/CD73 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4488, filled histogram) or control antibody (Catalog # 5‑001‑A, ...read more

Product Details

Summary
Reactivity Mu, PoSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

5'-Nucleotidase/CD73 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503
Specificity
Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NT5E
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF4488 in the following applications:

Publications
Read Publications using
AF4488 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5'-Nucleotidase/CD73 Antibody [Unconjugated]

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

  1. Resta, R. et al. (1993) Gene 133:171.
  2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
  3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5'-Nucleotidase/CD73 Antibody (AF4488)(2)

We have publications tested in 2 confirmed species: Mouse, Porcine.

We have publications tested in 2 applications: Flow Cytometry, IHC.


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Flow Cytometry
(1)
IHC
(1)
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Mouse
(1)
Porcine
(1)
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Reviews for 5'-Nucleotidase/CD73 Antibody (AF4488) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using AF4488:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IF Other 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23678122
SpeciesOther

Blocking

Blocking DetailsSee PMID 23678122

Details

Detection NotesSee PMID 23678122

Comments

CommentsPublished: See PMID 23678122Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22219293
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22219293

Details

Detection NotesSee PMID 22219293

Comments

CommentsPublished: See PMID 22219293Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for 5'-Nucleotidase/CD73 Antibody (AF4488). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF4488

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Blogs on 5'-Nucleotidase/CD73.

Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target
By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post.

CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
01/08/2015
Application: IF
Species: Other

 
Anonymous
01/08/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NT5E
Uniprot