5'-Nucleotidase/CD73 Antibody (4G4)


Flow Cytometry: CD73 Antibody (4G4) [NBP1-60135] - Flow cytometric detection of human CD73 on Huvec (mAb 4G4, Cat.# NBP1-60135). Black, red and blue line represents cells only, isotype control and 4G4 (4 ug/ml, ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IP
0.1 mg/ml

Order Details

5'-Nucleotidase/CD73 Antibody (4G4) Summary

Inflamed synovial stroma from rheumatoid arthitis patients
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
No Preservative
0.1 mg/ml
Protein A or G purified


  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:50
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody works in Flow Cytometry, Functional, Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Read Publications using
NBP1-60135 in the following applications:


Positive control: HUVEC and fresh lymphocytes Negative control: COS cells

Alternate Names for 5'-Nucleotidase/CD73 Antibody (4G4)

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase


The monoclonal antibody 4G4 recognizes both membrane bound and soluble human CD73, also known as ecto-5'-nucleotidase. CD73 is a 70-kDa GPI-anchored cell surface molecule and belongs to the 5'-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain subpopulations of human lymphocytes. Like many glycosyl-phosphatidylinositol (GPI)-anchored molecules, it transmits potent activation signals in T cells when ligated by antibodies. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Ecto-5'-nucleotidase activity is an important mediator of the anti-inflammatory effect by converting extracellular AMP into a potent anti-inflammatory substance adenosine. CD73 has been shown to function as a co-stimulatory molecule in human T cells and to have a role in regulating lymphocyte adhesion. Triggering of CD73 on the surface of lymphocytes, but not on endothelial cells, results in the shedding of the CD73 and increased adhesion of lymphocytes to endothelium via LFA-1 clustering. Furthermore, CD73 has been implicated to mediate homing of skin-infiltrating lymphocytes in vivo. In B-cell chronic lymphocytic leukemia the expression of CD73 is decreased. Besides this, CD73 activity has been implicated as sensitive and useful indicator for mild zinc deficiency. The monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes, reduces enzyme activity, and inhibits the binding of lymphocytes to endothelial cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5'-Nucleotidase/CD73 Antibody (NBP1-60135)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.

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Showing Publications 1 - 8 of 8.
Publications using NBP1-60135 Applications Species
Cinti A, De Giorgi M, Chisci E et al. Simultaneous Overexpression of Functional Human HO-1, E5NT and ENTPD1 Protects Murine Fibroblasts against TNF-a-Induced Injury In Vitro. PLoS ONE. 2015 Oct 30 [PMID:26513260] (ICC/IF, Human) ICC/IF Human
De Giorgi M, Cinti A, Pelikant-Malecka I et al. Co-expression of functional human Heme Oxygenase 1, Ecto-5'-Nucleotidase and ecto-nucleoside triphosphate diphosphohydrolase-1 by self-cleaving 2A peptide system. Plasmid. 2015 Mar 14 [PMID:25779031] (ICC/IF, Human) ICC/IF Human
Niemela J, Henttinen T, Yegutkin GG, Airas L, Kujari AM, Rajala P, Jalkanen S. IFN-alpha induced adenosine production on the endothelium: a mechanism mediated by CD73 (ecto-5'-nucleotidase) up-regulation. J Immunol;172(3):1646-53. 2004 Feb 1. [PMID:14734746]
Airas L, Niemela J, Jalkanen S. CD73 engagement promotes lymphocyte binding to endothelial cells via a lymphocyte function-associated antigen-1-dependent mechanism. J Immunol;165(10):5411-7. 2000 Nov 15. [PMID:11067892]
Airas L, Niemela J, Salmi M, Puurunen T, Smith DJ, Jalkanen S. Differential regulation and function of CD73, a glycosyl-phosphatidylinositol-linked 70-kD adhesion molecule, on lymphocytes and endothelial cells. J Cell Biol;136(2):421-31. 1997 Jan 27. [PMID:9015312]
Airas L, Salmi M, Jalkanen S. Lymphocyte-vascular adhesion protein-2 is a novel 70-kDa molecule involved in lymphocyte adhesion to vascular endothelium. J Immunol;151(8):4228-38. 1993 Oct 15. [PMID:8409398]
Airas L, Hellman J, Salmi M, Bono P, Puurunen T, Smith DJ, Jalkanen S. CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73. J Exp Med;182(5):1603-8. 1995 Nov 1. [PMID:7595232]
Cavalcanti BN. Pulp biology: aggression repair. Thesis. 2014 (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 5'-Nucleotidase/CD73 Antibody (NBP1-60135). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies


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Blogs on 5'-Nucleotidase/CD73.

CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.

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Gene Symbol NT5E

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