5'-Nucleotidase/CD73 Antibody (496406) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse 5'‑Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB4488) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 496406
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

5'-Nucleotidase/CD73 Antibody (496406) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
  Trp29-Lys549
  Accession # Q61503
• Specificity: Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: NT5E

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5'-Nucleotidase/CD73 Antibody (496406) [Unconjugated]

• 5' nucleotidase (CD73)
• 5'-NT
• 5-NT
• 5'-Nucleotidase
• 5'-nucleotidase, ecto (CD73)
• CD73 antigen
• CD73
• E5NT
• EC 3.1.3.5
• ecto-5'-nucleotidase
• eN
• eNT
• NT
• NT55'-nucleotidase
• NT5E
• NTE
• Purine 5-Prime-Nucleotidase

Background

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

1. Resta, R. et al. (1993) Gene 133:171.
2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for 5'-Nucleotidase/CD73 Antibody (MAB4488). (Showing 1 - 1 of 1 FAQ).

1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: NT5E
• Uniprot:
  • Q61503()