5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free

• Reactivity: Hu, Mu(-)
• Applications: Flow, ICC/IF, IHC, IP, Inhibition Activity
• Clone: 1E9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free Summary

• Description: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Highly purified human placental 5'-Nucleotidase/CD73 (Uniprot#: P21589)
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NT5E
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Inhibition Activity
• Application Notes: This antibody inhibits soluble placental 5'-NT enzymatic activity and recognizes lymphocyte CD73 in indirect immunofluorescence and immunoprecipitation assays. It is reported that 1E9 induces vigorous T cell proliferation in the presence of subatomic doses of phorbol myristate and F(ab')2 goat anti-mouse Ig

Reactivity Notes

This antibody does not show cross reactivity with mouse 5'-Nucleotidase/CD73

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free

• 5' nucleotidase (CD73)
• 5'-NT
• 5-NT
• 5'-Nucleotidase
• 5'-nucleotidase, ecto (CD73)
• CD73 antigen
• CD73
• E5NT
• EC 3.1.3.5
• ecto-5'-nucleotidase
• eN
• eNT
• NT
• NT55'-nucleotidase
• NT5E
• NTE
• Purine 5-Prime-Nucleotidase

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for 5'-Nucleotidase/CD73 Antibody (NBP3-45121). (Showing 1 - 1 of 1 FAQ).

1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: NT5E