17 beta-HSD1/HSD17B1 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

17 beta-HSD1/HSD17B1 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human HSD17B1.
  
  

  Source: E. coli

  Amino Acid Sequence: ALDPSQSFKVYATLRDLKTQGRLWEAARALACPQG

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: HSD17B1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-39053.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for 17 beta-HSD1/HSD17B1 Recombinant Protein Antigen

• 17 betaHSD1
• 17 beta-HSD1
• 17-beta-HSD 1
• 17-beta-hydroxysteroid dehydrogenase type 1
• 20 alpha-hydroxysteroid dehydrogenase
• 20-alpha-HSD
• E17KSR
• E2DH
• EC 1.1.1.62
• EDH17B1
• EDH17B2
• EDH17B2EDHB17
• EDHB17
• estradiol 17-beta-dehydrogenase 1
• estradiol 17-beta-dehydrogenase-1
• HSD17
• HSD17B1
• hydroxysteroid (17-beta) dehydrogenase 1 isoform
• hydroxysteroid (17-beta) dehydrogenase 1
• MGC138140
• Placental 17-beta-hydroxysteroid dehydrogenase
• SDR28C1
• short chain dehydrogenase/reductase family 28CE, member 1

Background

The principal human estrogen, 17 beta-estradiol, is a potent stimulator of certain endocrine-dependent forms of breast cancer. Because human estrogenic 17 beta-hydroxysteroid dehydrogenase (HSD17B1) catalyzes the last step in the biosynthesis of 17 beta-estradiol from the less potent estrogen, estrone, it is an attractive target for the design of inhibitors of estrogen production and tumor growth (1). It is concluded that the steroid-binding site of human placental HSD17B1 contains a histidine residue which proximates the upper A-ring region of the steroid as it undergoes the reversible binding step (2). Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies (3). Defects in the conversion of androstenedione to testosterone in the fetal testes by the enzyme HSD17B1 give rise to genetic males with female external genitalia (4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HSD17B1