CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glomeruli and tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Renin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Renin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glomeruli and tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4277-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4090-SP | |||
CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Renin was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Mouse Anti-Human Renin Propeptide Monoclonal Antibody (Catalog # MAB4447) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4447-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73851 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89723-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4277-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4090-SP | |||
4277-AS) was serially diluted and captured by Goat Anti-Mouse Renin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4277) coated on a Clear Polystyrene Microplate (Catalog # 
4277-AS) was serially diluted and captured by Goat Anti-Mouse Renin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # 
![Western Blot: Renin Antibody (2F10) [NBP2-45552] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Renin.](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Western-Blot-NBP2-45552-img0010.jpg "Western Blot: Renin Antibody (2F10) [NBP2-45552] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Renin.")
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Immunohistochemistry-NBP2-45552-img0009.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Immunohistochemistry-NBP2-45552-img0001.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Immunohistochemistry-NBP2-45552-img0002.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Immunohistochemistry-NBP2-45552-img0003.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Immunohistochemistry-NBP2-45552-img0004.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Immunohistochemistry-NBP2-45552-img0005.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Immunohistochemistry-NBP2-45552-img0006.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Immunohistochemistry-NBP2-45552-img0007.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Renin-Antibody-2F10-Immunohistochemistry-NBP2-45552-img0008.jpg)
![N/A Renin [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Renin_DY4277_ELISA_2022.jpg "N/A Renin [Biotin]")
![N/A Renin [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Renin_DREN00_ELISA_805.jpg "N/A Renin [HRP]")
![N/A Renin [HRP]](http://images.novusbio.com/fullsize/elisa/Renin_DREN00_ELISA_170.jpg)
![Immunohistochemistry-Paraffin: Renin Antibody (009) [NBP2-89723] - Staining of human RENIN in human kidney with rabbit monoclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Renin-Antibody-009-Immunohistochemistry-Paraffin-NBP2-89723-img0002.jpg "Immunohistochemistry-Paraffin: Renin Antibody (009) [NBP2-89723] - Staining of human RENIN in human kidney with rabbit monoclonal antibody at 1:1000 dilution.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Renin Antibody (009) [NBP2-89723] - Analysis of Human Renin expression on Jurkat cells. The cells were treated , stained with purified anti-Human Renin, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Renin-Antibody-009-Flow-Cytometry-NBP2-89723-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Renin Antibody (009) [NBP2-89723] - Staining of Human Renin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89723 antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89723_rabbit-monoclonal-renin-antibody-009-23520251664742.png)
![N/A Renin [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Renin_DY4090_ELISA_2007.jpg "N/A Renin [Biotin]")
![ELISA: Rat Renin ELISA Kit (Colorimetric) [NBP2-80264] - Samples were spiked with high concentrations of Rat Renin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80264_rat-renin-elisa-kit-colorimetric-13220241613065.jpg "ELISA: Rat Renin ELISA Kit (Colorimetric) [NBP2-80264] - Samples were spiked with high concentrations of Rat Renin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Renin ELISA Kit (Colorimetric) [NBP2-80264] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80264_rat-renin-elisa-kit-colorimetric-4320249115697.jpg)
![Western Blot: Renin Antibody (2H2) [H00005972-M01] - REN monoclonal antibody (M01), clone 2H2 Analysis of REN expression in K-562.](http://images.novusbio.com/fullsize/Renin-Antibody-2H2-Western-Blot-H00005972-M01-img0004.jpg "Western Blot: Renin Antibody (2H2) [H00005972-M01] - REN monoclonal antibody (M01), clone 2H2 Analysis of REN expression in K-562.")
![Sandwich ELISA: Renin Antibody (2H2) [H00005972-M01] - Detection limit for recombinant GST tagged REN is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Renin-Antibody-2H2-Sandwich-ELISA-H00005972-M01-img0003.jpg)
