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Rat Renin ELISA Kit (Colorimetric)

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ELISA: Rat Renin ELISA Kit (Colorimetric) [NBP2-80264] - Standard Reference Curve
ELISA: Rat Renin ELISA Kit (Colorimetric) [NBP2-80264] - Standard Reference Curve

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.625-1000 pg/mL
Sensitivity
9.375 pg/mL

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Rat Renin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat REN in samples. No significant cross-reactivity or interference between Rat REN and analogues was observed.
Standard Curve Range
15.625-1000 pg/mL
Sensitivity
9.375 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.04%
Intra-Assay
CV% < 5.13%
Spike Recovery
88-110%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
REN

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100X)
  4. Concentrated HRP Conjugate (100X)
  5. Concentrated Wash Buffer (25X)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard and Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Rat Renin ELISA Kit (Colorimetric)

  • angiotensin-forming enzyme
  • Angiotensinogenase
  • EC 3.4.23
  • EC 3.4.23.15
  • FLJ10761
  • HNFJ2
  • Prorenin
  • REN
  • renin precursor, renal
  • Renin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.
Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody - BSA Free [NBP1-30027] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of Serpin A8 (NBP1-30027) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
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ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15&amp;#160;&amp;micro;g/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4&amp;#160;&amp;deg;C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05&amp;nbsp;µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). A specific band was detected for ACE/CD143 at approximately 180&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
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Rat Renin ELISA
Kit (Colorimetric)
NBP2-80264
Rat Renin ELISA Kit (Colorimetric)
Species: Rt
Applications: ELISA

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Bioinformatics

Gene Symbol REN
Uniprot