Mouse Renin 1 DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Renin 1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ren1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Renin 1 DuoSet ELISA, 15 Plate

• angiotensin-forming enzyme
• Angiotensinogenase
• EC 3.4.23
• EC 3.4.23.15
• FLJ10761
• HNFJ2
• Prorenin
• REN
• renin precursor, renal
• Renin

Background

Renin is a secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. The 406 aa precursor includes a signal peptide (aa 1 - 23), a propeptide (aa 24 - 66), and a mature chain (aa 67 - 406). The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat.

Mouse Renin is a secreted, 42 - 47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22 - 402). In the kidney, prorenin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro-renin shares 70% and 85% aa sequence identity with human and rat pro-renin, respectively.

Publications for Renin (DY4277)(3)

Publications using DY4277

• Berger, JH;Shi, Y;Matsuura, TR;Batmanov, K;Chen, X;Tam, K;Marshall, M;Kue, R;Patel, J;Taing, R;Callaway, R;Griffin, J;Kovacs, A;Hirenallur-Shanthappa, D;Miller, R;Zhang, BB;Flach, RJR;Kelly, DP; Two-hit mouse model of heart failure with preserved ejection fraction combining diet-induced obesity and renin-mediated hypertension Scientific reports 2025-01-02 [PMID: 39747575] (Mouse)
• LY Herat, AL Magno, C Rudnicka, J Hricova, R Carnagarin, NC Ward, A Arcambal, MG Kiuchi, GA Head, MP Schlaich, VB Matthews SGLT2 Inhibitor-Induced Sympathoinhibition: A Novel Mechanism for Cardiorenal Protection JACC Basic Transl Sci, 2020-01-29;5(2):169-179. 2020-01-29 [PMID: 32140623] (Mouse)
• Harlan S, Ostroski R, Coskun T, Yantis L, Breyer M, Heuer J Viral transduction of renin rapidly establishes persistent hypertension in diverse murine strains. Am J Physiol Regul Integr Comp Physiol, 2015-06-24;309(5):R467-74. 2015-06-24 [PMID: 26108870] (Mouse)

FAQs for Renin (DY4277). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Ren1