Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-54578SS | |||
| Azide and BSA Free | NBP2-81001 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56507SS | |||
| Azide and BSA Free | NBP2-80787 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24683SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-89062SS | |||
| Azide and BSA Free | NBP2-80895 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-524SS | |||
| Azide and BSA Free | NBP2-80892 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-56893SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33337SS | |||
| Azide and BSA Free | NBP2-80656 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1992SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-127SS | |||
![Immunohistochemistry-Frozen: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP in mouse brain. Antibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100. Image from verified customer review.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Immunohistochemistry-Frozen-NBP1-54578-img0007.jpg "Immunohistochemistry-Frozen: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP in mouse brain. Antibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100. Image from verified customer review.")
![Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP expression in Raji (1), K562 (2), KG1 (3), MRC5 (4), IC2Tr (5), HEL (6), P19 (7) and WR19L (8).](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Western-Blot-NBP1-54578-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] - Analysis of TXNIP (NBP1-54578), ACTB, CASP1 (cleaved), and IL1B (cleaved) protein in livers from mice treated with WY-14643 for 48 h. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33203882/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Azide-and-BSA-Free-Western-Blot-NBP1-54578-img0011.jpg)
![Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] - An intracellular stain was performed on THP-1 cells with TXNIP Antibody (JY2) NBP1-54578AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Azide-and-BSA-Free-Flow-Cytometry-NBP1-54578-img0010.jpg)
![Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - Hepa1 lysates probed with anti-TXNIP (JY2) at 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged). 30ug protein per lane. Image from verified customer review.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Western-Blot-NBP1-54578-img0006.jpg)
![Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - AMPK mediates anti-pyroptotic effects of Exendin-4. (a) ROS determination by FACS. The left arrow indicated the ROS-negative population and the right pointed the positive. (b) Transcription activity of TXNIP in cardiomyocytes. (c) Western blot. (d) RNA silencing of TXNIP in cardiomyocytes. (e) IL-1 ELISA with TXNIP RNAi. (f) Caspase-1 activity assay with TXNIP RNAi. (g) Quantification of pAMPK/AMPK with CC treatment. (h) IL-1 ELISA with CC treatment. (i) Caspase-1 activity assay with CC treatment. Values are the mean SEM of 3 samples per group.0.05,0.01,0.005, and0.001. Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway. J Diabetes Res (2019)](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Western-Blot-NBP1-54578-img0009.jpg)
![Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:250 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with hematoxylin. This TXNIP antibody generated a diffused cytoplasmic signal in the cells of various tubules and glomeruli.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Immunohistochemistry-Paraffin-NBP1-54578-img0003.jpg)
![Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with hematoxylin. This TXNIP antibody generated a diffused cytoplasmic signal in the cells of various tubules with negligible staining in glomeruli.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Immunohistochemistry-Paraffin-NBP1-54578-img0005.jpg)
![Flow Cytometry: TXNIP Antibody (JY2) [NBP1-54578] - An intracellular stain was performed on RH-30 cells with TXNIP (JY2) Antibody NBP1-54578F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Flow-Cytometry-NBP1-54578-img0008.jpg)
![Immunoprecipitation: TXNIP Antibody (JY2) [NBP1-54578] - Txnip/VDUP1 from Raji with NBP1-54578 (1) or mouse IgG1 (2).](http://images.novusbio.com/fullsize/TXNIP-Antibody-JY2-Immunoprecipitation-NBP1-54578-img0002.jpg)
![Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] - AMPK mediates anti-pyroptotic effects of Exendin-4. (a) ROS determination by FACS. The left arrow indicated the ROS-negative population & the right pointed the positive. (b) Transcription activity of TXNIP in cardiomyocytes. (c) Western blot. (d) RNA silencing of TXNIP in cardiomyocytes. (e) IL-1 beta ELISA with TXNIP RNAi. (f) Caspase-1 activity assay with TXNIP RNAi. (g) Quantification of pAMPK/AMPK with CC treatment. (h) IL-1 beta ELISA with CC treatment. (i) Caspase-1 activity assay with CC treatment. Values are the mean ± SEM of 3 samples per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, & ∗∗∗∗p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31886288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54578_mouse-monoclonal-txnip-antibody-jy2-310202415384194.jpg)
![Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Knockout-Validated-NB100-56507-img0032.jpg "<b>Genetic Strategies Validation. </b>Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIHx2011;3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 ug/mL mouse anti-IkB-a monoclonal (NB100-56507, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Western-Blot-NB100-56507-img0031.jpg)
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (6A920) [NB100-56507] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IkB-alpha (6A920) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Immunocytochemistry-Immunofluorescence-NB100-56507-img0035.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha Antibody (6A920) [NB100-56507] - Analysis of a FFPE tissue section of human breast using 1:200 dilution of IkB-alpha clone 6A920 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and membrane staining of glandular cells was observed.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Immunohistochemistry-Paraffin-NB100-56507-img0036.jpg)
![Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - An intracellular stain was performed on NIH3T3 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Flow-Cytometry-NB100-56507-img0034.jpg)
![Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - IkBa using NB100-56507 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Western-Blot-NB100-56507-img0027.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NB100-56724 (lanes 1 & 2) or NB100-56507 (that recognizes both non-phospho and phosphorylated forms of IkBa) and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Western-Blot-NB100-56507-img0028.jpg)
![Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - Intracellular staining of 10^6 ThP-1 cells using 0.25 ug of NB100-56507. Shaded histogram represents cells alone, green represents the isotype control, and red represents the IkBa antibody. Novus's intracellular flow kit was used for this test, and an anti-mouse IgG FITC conjugated secondary.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Flow-Cytometry-NB100-56507-img0025.jpg)
![Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - An intracellular stain was performed on Hek293 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Flow-Cytometry-NB100-56507-img0033.jpg)
![Simple Western: IkB-alpha Antibody (6A920) [NB100-56507] - Simple Western lane view shows a specific band for IKB alpha in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Simple-Western-NB100-56507-img0024.jpg)
![Western Blot: STING/TMEM173 Antibody [NBP2-24683] - STING/TMEM173 expression was induced in human breast MDA-MB-231 cells followed by Western blotting using STING/TMEM173 Antibody antibody (1:1000). Only one specific band at an apparent molecular mass of 37 kDa was observed. Image from verified customer review.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Western-Blot-NBP2-24683-img0014.jpg "Western Blot: STING/TMEM173 Antibody [NBP2-24683] - STING/TMEM173 expression was induced in human breast MDA-MB-231 cells followed by Western blotting using STING/TMEM173 Antibody antibody (1:1000). Only one specific band at an apparent molecular mass of 37 kDa was observed. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - RH-30 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with STING/TMEM173 Antibody at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha Tubulin Antibody (DM1A) (NB100-690) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24683-img0017.jpg)
![Western Blot: STING/TMEM173 Antibody [NBP2-24683] - Total protein from THP-1, HT-29, U2OS cells and human spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml STING/TMEM173 Antibody in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Western-Blot-NBP2-24683-img0011.jpg)
![Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Human colon cancer tissue section using STING/TMEM173 Antibody at 1:100 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated very weak cytoplasmic staining in columnar epithelial cells with a very strong signal in the secretory/goblet cells.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Immunohistochemistry-Paraffin-NBP2-24683-img0006.jpg)
![Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Mouse lung tissue section using STING/TMEM173 Antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated mainly a cytoplasmic staining in the bronchiolar and alveolar epithelial cells.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Immunohistochemistry-Paraffin-NBP2-24683-img0007.jpg)
![Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on U937 cells with NBP2-24683AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Flow-Cytometry-NBP2-24683-img0020.jpg)
![Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody NBP2-24683AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody---BSA-Free-Flow-Cytometry-NBP2-24683-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - HT-29 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with STING/TMEM173 Antibody at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24683-img0015.jpg)
![Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Flow-Cytometry-NBP2-24683-img0012.jpg)
![Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on RH30 cells with STING/TMEM173 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Flow-Cytometry-NBP2-24683-img0016.jpg)
![Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of 293T cells using Centrin 3 antibody (3E6). Image from verified customer review.](http://images.novusbio.com/fullsize/Centrin-3-Antibody-3E6-Western-Blot-H00001070-M01-img0011.jpg "Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of 293T cells using Centrin 3 antibody (3E6). Image from verified customer review.")
![Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - CETN3 monoclonal antibody (M01), clone 3E6 Analysis of CETN3 expression in Jurkat.](http://images.novusbio.com/fullsize/Centrin-3-Antibody-3E6-Western-Blot-H00001070-M01-img0007.jpg)
![Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of CETN3 expression in transfected 293T cell line by CETN3 monoclonal antibody (M01), clone 3E6.Lane 1: CETN3 transfected lysate(19.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Centrin-3-Antibody-3E6-Western-Blot-H00001070-M01-img0008.jpg)
![Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of CETN3 over-expressed 293 cell line, cotransfected with CETN3 Validated Chimera RNAi ( Cat # H00001070-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CETN3 monoclonal antibody (M01), clone 3E6 (Cat # H00001070-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Centrin-3-Antibody-3E6-Western-Blot-H00001070-M01-img0010.jpg)
![ELISA: Centrin 3 Antibody (3E6) [H00001070-M01] - Centrin 3 Antibody (3E6) [H00001070-M01] - Detection limit for recombinant GST tagged CETN3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Centrin-3-Antibody-3E6-ELISA-H00001070-M01-img0009.jpg)
![Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Western-Blot-NB110-89062-img0007.jpg "Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89062-img0008.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Immunohistological results at 12 weeks for (A) Group I, (B) Group II (C) Group III.The pictures are arranged by staining technique (columns) and by the investigated treatment (rows). Areas that stained positive for osteopontin are indicated by red arrowheads. Image collected and cropped by CiteAb from the following publication (//peerj.com/articles/3513) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunohistochemistry-NB110-89062-img0012.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Osteogenic differentiation in 3D. Immunostaining for osteopontin (OPN) and nuclear DAPI in 3D cultures after 5 weeks. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34948393/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunohistochemistry-NB110-89062-img0013.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Analysis of Osteopontin on human lung adenocarcinoma.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunohistochemistry-NB110-89062-img0009.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks. The pictures are arranged by staining technique (columns) and the investigated treatment (rows). Areas that stained positive for osteocalcin and osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected and cropped by CiteAb from the following publication (//peerj.com/articles/3513) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunohistochemistry-NB110-89062-img0011.jpg)
![Simple Western: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Simple-Western-NB110-89062-img0010.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Photomicrographs of immunostaining for osteocalcin & osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) & the investigated treatment (rows). Areas that stained positive for osteocalcin & osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected & cropped by CiteAb from the following publication (//peerj.com/articles/3513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89062_mouse-monoclonal-osteopontin-opn-antibody-1b20-3102024153560.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Photomicrographs of immunostaining for osteocalcin & osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) & the investigated treatment (rows). Areas that stained positive for osteocalcin & osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected & cropped by CiteAb from the following publication (//peerj.com/articles/3513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89062_mouse-monoclonal-osteopontin-opn-antibody-1b20-31020241534321.jpg)
![Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Photomicrographs of immunostaining for osteocalcin & osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) & the investigated treatment (rows). Areas that stained positive for osteocalcin & osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected & cropped by CiteAb from the following publication (//peerj.com/articles/3513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89062_mouse-monoclonal-osteopontin-opn-antibody-1b20-310202415394515.jpg)
![Immunohistochemistry-Paraffin: O-GlcNAc Antibody (RL2) [NB300-524] - Analysis of a FFPE tissue section of the mouse colon using 1:200 dilution of O-GlcNAc [RL2] antibody (NB300-524). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Immunohistochemistry-Paraffin-NB300-524-img0011.jpg "Immunohistochemistry-Paraffin: O-GlcNAc Antibody (RL2) [NB300-524] - Analysis of a FFPE tissue section of the mouse colon using 1:200 dilution of O-GlcNAc [RL2] antibody (NB300-524). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.")
![Western Blot: O-GlcNAc Antibody (RL2) [NB300-524] - Impact of OGA inhibition by thiamet G in primary cultures of NCM. Representative western blots (left panel) and quantification (right panel) of O-GlcNAcylated proteins levels in control (Ctrl) and NCM treated with 100 nM of thiamet G (TG) during 24 h (n = 12). Image collected and cropped by Citeab from the following publication (Interplay Between Phosphorylation and O-GlcNAcylation of Sarcomeric Proteins in Ischemic Heart Failure. Front Endocrinol (Lausanne) (2018) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Western-Blot-NB300-524-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (RL2) [NB300-524] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-O-GlcNAc (RL2) at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Immunocytochemistry-Immunofluorescence-NB300-524-img0013.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on Neuro2a cells with O-GlcNAc Antibody [RL2] NB300-524 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0018.jpg)
![Western Blot: O-GlcNAc Antibody (RL2) [NB300-524] - Analysis of mouse cortical brain lysates using O-Linked N-Acetylglucosamine Monoclonal Antibody. Blots containing cortical extracts from 4 individual C57BL/6 mice (Lanes 1-4) were blocked with 5% milk in TBST, and probed with MA1-072 at 1:1000, followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Western-Blot-NB300-524-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (RL2) [NB300-524] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-O-GlcNAc (RL2) at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Immunocytochemistry-Immunofluorescence-NB300-524-img0012.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - Analysis using Alexa Fluor (R) 647 conjugate of NB300-524. An intracellular stain was performed on Jurkat cells with O-GlcNAc antibody (RL2) NB300-524 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0006.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on U-937 cells with O-GlcNAc antibody (RL2) NB300-524AF647 (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature . Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0007.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on Jurkat cells with O-GlcNAc antibody (RL2) NB300-524PE (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature . Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0008.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on SK-MEL-28 cells with O-GlcNAc antibody (RL2) NB300-524AF647 (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature . Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0009.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on HeLa cells with O-GlcNAc Antibody [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0010.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on Neuro2a cells with O-GlcNAc Antibody [RL2] NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0014.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on RH30 cells with O-GlcNAc [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0015.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on Jurkat cells with O-GlcNAc Antibody [RL2] NB300-524 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0017.jpg)
![Western Blot: O-GlcNAc Antibody (RL2) - BSA Free [NB300-524] - Analysis of O-GlcNAcylated LV proteins by Western blot & WGA-SDS-PAGE gel electrophoresis. (A) Red ponceau staining (left panel) & western blot (right panel) of O-GlcNAcylated proteins (50 μg) extracted from sham- & HF-rats treated or not with thiamet G. The positions of molecular weight are indicated as kilodalton (kDa) on the left. (B) Red ponceau staining (left panel) & WGA-SDS-PAGE of O-GlcNAcylated proteins levels (middle panel) of O-GlcNAcylated desmin levels (right panel) from the same samples. The arrow in desmin WGA gels indicates the non-O-GlcNAcylated form. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30344511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-524_mouse-monoclonal-o-glcnac-antibody-rl2-310202415394544.jpg)
![Western Blot: O-GlcNAc Antibody (RL2) - BSA Free [NB300-524] - Effect of OGA inhibition by thiamet G in isolated perfused heart. (A) Description of the protocol designed for thiamet G (TG) perfusion in sham- (n = 6) & HF- (n = 7) rats 6 weeks post-MI. (B) Western blot (left panel) & quantification (right panel) of O-GlcNAcylated proteins levels measured in proteins extracted from LVs of isolated perfused sham- & HF-rat hearts treated or not with 100 μM thiamet G for 2 h (n = 7 in each group). (C) Western blots (upper panel) & quantification (lower panel) of total desmin levels in the same samples. (D) Phosphorylation profiles of desmin were analyzed in the same samples by Phos-tag™ gel. Graphs show mean ± SEM values expressed in arbitrary units (A.U.). The positions of molecular weight are indicated as kilodalton (kDa) on the left. *P < 0.05; ** < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30344511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-524_mouse-monoclonal-o-glcnac-antibody-rl2-31020241621845.jpg)
![Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used at 0.1 ug/ml. Lower panel: Rabbit anti-RPA32 antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0047.jpg "Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used at 0.1 ug/ml. Lower panel: Rabbit anti-RPA32 antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and EPE treated cells (right). Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor (R) 488 phalloidin (green).](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-23017-img0046.jpg)
![Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of mouse gut, MOCK treatment (left) and CIP treatment (right).Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunohistochemistry-Paraffin-NBP1-23017-img0050.jpg)
![Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Samples: Whole cell lysate (50 ug for WB in A; 1 mg for IP, 20% of IP loaded in B) from HeLa Cells that had been treated with EPE (+) or mock treated (-). In B, immunoprecipitated RPA32 was either treated with phosphatase (+) or mock treated (-). Antibodies: Phosphorylated RPA32 was detected using rabbit anti-phospho RPA32 Ser4/Ser8 antibody NBP1-23017 lot 2 at 1 ug/ml. Total RPA32 was detected using rabbit anti-RPA32 antibody NB100-332 at 1 ug/ml. RPA32 was immunoprecipitated using NB100-332 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds for phospho RPA32 Ser4/Ser8 (A and B) and 1 second for total RPA32 (A and B).](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows Westen Blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 ug/ml with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0042.jpg)
![Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of human breast carcinoma, MOCK treatment (left) and CIP treatment(right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) (used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunohistochemistry-Paraffin-NBP1-23017-img0049.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with EPE (+) and/or Lambda PP (+) or mock treated (-). Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-RPA32 antibody NB100-332. For blotting immunoprecipitated Phospho RPA32 (S4/S8), rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 was used at 0.1 ug/ml. Lower Panel: Rabbit anti-RPA antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunoprecipitation-NBP1-23017-img0048.jpg)
![Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. (1) siRNA ATG9A knockdown and (2) wildtype ATG9A.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Western-Blot-NB110-56893-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. (1) siRNA ATG9A knockdown and (2) wildtype ATG9A.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody [NB110-56893] - After stimulation with the NOD1 ligand, macrophages increased their expression of autophagy proteins Atg9, LC3 and IRGM. Alveolar macrophages (AMs), monocyte-derived macrophages (MDMs) and monocytes (MNs) were incubated in the presence of 5 ug/ml of Tri-DAP for 24 h. Cells were pre-incubated with Rip2/p38 inhibitor SB203580 (SB) for 30 min prior to Tri-DAP stimulation to block NOD1-mediated responses, as indicated. Atg9 and LC3 proteins were measured in the cytosolic fractions by western blot analysis. Image collected and cropped by CiteAb from the following publication (//bmcpulmmed.biomedcentral.com/articles/10.1186/1471-2466-14-152), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Western-Blot-NB110-56893-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody [NB110-56893] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-ATG9A at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-56893-img0008.jpg)
![Immunohistochemistry: ATG9A Antibody [NB110-56893] - Staining in mouse intestine.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunohistochemistry-NB110-56893-img0005.jpg)
![Flow Cytometry: ATG9A Antibody [NB110-56893] - An intracellular stain was performed on Hela Cells with NB110-56893 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).](http://images.novusbio.com/fullsize/ATG9A-Antibody-Flow-Cytometry-NB110-56893-img0007.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of chloroquine, spautin-1 & MG132 on ATG protein levels in A549 cells. (A) A549 cells were treated with 50 μM chloroquine, 10 μM spautin-1 or 5 μM MG132 in the presence or absence of 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3, mean±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415371977.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - CD133 promoted glucose uptake.A. Levels of glucose uptake in Huh-7con & Huh-7sh133 cells. Mean fluorescence intensity of 2-NBDG uptake was measured by FASC analysis after incubating for 6 h. B. Time course of 2-NBDG uptake in these two cell lines. Statistical significance indicated as *p<0.05; **p<0.01. C. CD133 or empty vector was transfected respectively into LM3 cells. Glucose uptake was measured at the indicated times upon the medium added with 100 µM 2-NBDG. *p<0.05. D. Detection of relative activities of signaling pathways in Huh-7con & Huh-7sh133 cells by Western blotting. con: Huh-7con cells; sh: Huh-7sh133 cells. E. Detection of autophagic genes in Huh-7con & Huh-7sh133 cells by Western blotting. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437259), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415382433.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of TGF beta 1 on ATG protein levels & LC3B lipidation in A549 cells. (A) A549 cells were treated with 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415384151.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Mouse spleen cryosection.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Immunohistochemistry-Paraffin-NBP2-33337-img0009.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Mouse spleen cryosection.")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Mouse spleen.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Immunohistochemistry-Paraffin-NBP2-33337-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Wehi-3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti CD68 (FA-11) [NBP2-33337] at a 1:100 dilution overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Immunocytochemistry-Immunofluorescence-NBP2-33337-img0012.jpg)
![Flow (Intracellular): CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - An intracellular stain was performed on Raw 246.7 cells with CD68/SR-D1 (FA-11) antibody NBP2-33337 (blue) and a matched isotype control MAB006 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by DyLight488-conjugated anti-rat secondary antibody.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Flow-Intracellular-NBP2-33337-img0011.jpg)
![Western Blot: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Expression on J774 cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Western-Blot-NBP2-33337-img0010.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - An intracellular stain was performed on Raw264.7 cells with CD68/SR-D1 Antibody (FA-11) NBP2-33337B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Flow-Cytometry-NBP2-33337-img0013.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (FA-11) [NBP2-33337] - Staining of permeabilised Mouse peritoneal Macrophages cells with Rat anti Mouse CD68 Antibody (Clone FA-11) visualised with F(ab')2 Goat Anti Rat IgG:FITC (Mouse Adsorbed).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-FA-11-Flow-Cytometry-NBP2-33337-img0007.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, and protein expression of oncogenes and tumor suppressive genes in 1alpha(OH)ase -/- mice. Mice from each group were treated as described in Figure 1. Representative micrographs of skin sections stained immunohistochemically for SOD2 and gamma-H2AX. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.12951), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-NB100-1992-img0013.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, and protein expression of oncogenes and tumor suppressive genes in 1alpha(OH)ase -/- mice. Mice from each group were treated as described in Figure 1. Representative micrographs of skin sections stained immunohistochemically for SOD2 and gamma-H2AX. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.12951), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1992-img0010.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Western blot analysis of Rat Tissue lysates showing detection of SOD2/Mn-SOD protein using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NB100-1992-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1992-img0012.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - Immunohistochemistry analysis using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-NB100-1992-img0017.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-31020241555245.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-3102024168392.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-31020241553748.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - CAV-1 preserves mitochondrial respiratory function through regulation of OXPHOS expression.(A) OXPHOS complex subunits were detected through western blotting with appropriate antibodies. CAV-1 treatment significantly restored hypercholesterolemia-associated increased ETC complex I–V proteins levels. (B) Percentage of the OXPHOS complex (I–V) band intensities is presented in the graph. (C) Representative immunoblot displaying levels of CAV-1, CyPA, mitochondrial biogenesis markers, & antioxidant enzymes in the NC, HC, & HCAV-1 groups. (D) Columns represent average values over three independent experiments. The density for the NC group was set at 1; * & † are significantly different for the NC & HC groups, respectively, at P < 0.05. beta -actin was used as a loading control. Values are means ± SD. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154210), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-3102024155523.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-310202415541978.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-310202415535131.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-10-34), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1992_rabbit-polyclonal-sod2-mn-sod-antibody-3102024165716.jpg)
![Western Blot: DDX6 Antibody [NB200-191] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-NB200-191-img0014.jpg "Western Blot: DDX6 Antibody [NB200-191] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunocytochemistry-Immunofluorescence-NB200-191-img0008.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NB200-191-img0013.jpg)
![Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunoprecipitation-NB200-191-img0012.jpg)
![Western Blot: DDX6 Antibody [NB200-191] - Staufen1 protein but not mRNA steady-state levels are increased in neurodegenerative disease cells & tissues. Western blot analysis of SCA2- FBs (a) & LBCs (b) show increased STAU1 levels compared with normal controls. DDX6 levels are unchanged. HD & SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal & five SCA2 FBs, & two normal & three SCA2 LBCs were used. c, d Western blot analyses of ATXN2Q127 (c) & BAC-Q72 (d) mouse cerebellar extracts (24 weeks of age) showing increased Stau1 levels compared with wild-type or BAC-Q22 controls (n = 2–3 animals per group). e Western blot of FB extracts from an ALS patient with the TDP-43G298S mutation show increased STAU1 levels. beta -Actin was used as loading control & representative blots of three independent experiments are shown. f–hSTAU1 RNA levels are unaltered in SCA2 & ALS cells & SCA2 mice. qRT-PCR analyses of STAU1 mRNA in SCA2 FBs & ALS FB with TDP-43G298S mutation (f) or SCA2 LBCs (g). h qRT-PCR analyses of cerebellar RNAs from ATXN2Q127 & BAC-Q72 mice compared to wild-type littermates (24 weeks of age; n = animals per group). Gene expression levels were normalized to Actb Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-191_rabbit-polyclonal-ddx6-antibody-310202416212368.jpg)
![Immunohistochemistry: AIP/ARA9 Antibody (35-2) [NB100-127] - Fig 1. Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A and B Examples of low AIP expression; C and D: Examples of high AIP expression; E Normal human pituitary staining with omitting primary antibody (negative control); F Normal human pituitary staining with AIP (positive control); Scale bar = 1000 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117107) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-35-2-Immunohistochemistry-NB100-127-img0013.jpg "Immunohistochemistry: AIP/ARA9 Antibody (35-2) [NB100-127] - Fig 1. Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A and B Examples of low AIP expression; C and D: Examples of high AIP expression; E Normal human pituitary staining with omitting primary antibody (negative control); F Normal human pituitary staining with AIP (positive control); Scale bar = 1000 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117107) licensed under a CC-BY license.")
![Simple Western: AIP/ARA9 Antibody (35-2) [NB100-127] - Simple Western lane view shows a specific band for AIP/ARA9 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-35-2-Simple-Western-NB100-127-img0009.jpg)
![Western Blot: AIP/ARA9 Antibody (35-2) [NB100-127] - Detection on HEK 293T/17 cells. Lane 1: IP ARA9, WB ARA9, Lane 2: WB ARA9 with whole cell lysate. Photo courtesy of Liling Zeng, Boston University.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-35-2-Western-Blot-NB100-127-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AIP/ARA9 Antibody (35-2) [NB100-127] - Effect of miR-34a (A) and anti-miR-34a (B) on endogenous AIP protein levels in HEK293 and in GH3 (C) cells 48 hours after transfection. Data are shown as mean +/- SEM, **, P<0.01 ***, P<0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25658813/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-35-2-Western-Blot-NB100-127-img0014.jpg)
![Flow Cytometry: AIP/ARA9 Antibody (35-2) [NB100-127] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using AIP/ARA9 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-35-2-Flow-Cytometry-NB100-127-img0008.jpg)
![Immunohistochemistry-Paraffin: AIP/ARA9 Antibody (35-2) [NB100-127] - AIP/ARA9 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human AIP/ARA9 (35-2) Monoclonal Antibody (Catalog # NB100-127) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in splenocytes.](http://images.novusbio.com/fullsize/AIP-ARA9-Antibody-35-2-Immunohistochemistry-Paraffin-NB100-127-img0010.jpg)
![EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(A) Cartoon of human EPS15 domain-organization and the amino-acid sequence. Indicated by color code are the SPOP/SPOPL binding site (red) and the lysine residue (yellow), which is ubiquitinated in a CRL3SPOPL–dependent manner in vivo. In addition, the amino-terminal Ca2+-binding EF-hand motifs (EH), the coiled-coil domain involved in dimerization and the two carboxy-terminal ubiquitin-interacting motifs (UIMs) involved in ubiquitin-binding are indicated. (B) EPS15 ubiquitin-profiling. Peptides containing EPS15 modification sites were quantified with LC-MS/MS after enrichment of the K-epsilon -GG motif from whole cell digests of HeLa cells treated with siSPOPL or siControl. Normalized precursor mass intensity profiles for EPS15 sites corresponding to K793, K801 and K693 are shown (raw data in Figure 4—figure supplement 1B). Quantification of the beta -Actin K113 and the polyubiquitin K11 linkage peptide control for comparable enrichment. Data are mean ± SD, N = 3. **p≤0.01. (C) Purified SPOPL was incubated as indicated with GST-tagged wild-type EPS15 or GST-EPS15 mutants, where the predicted SPOPL binding motifs have been mutated individually (GST-EPS15S605-S607A and EPS15S744-S746A, respectively), pulled down with glutathione sepharose (IP [GST]) and bound proteins were analyzed by Coomassie blue staining (upper panel) and immunoblotting (lower panels). Note that SPOPL readily binds to GST-EPS15 and GST-EPS15S605-S607A, but this interaction is strongly reduced with the GST-EPS15S744-S746A mutant. (D) HeLa cells stably expressing GFP-tagged wild-type EPS15, the EPS15S744-S746A or the EPS15K793R mutants from a doxycycline-inducible promoter were transfected as indicated (+) with control siRNA or siRNA depleting SPOPL. The levels of EPS15-GFP, EGFR and for control tubulin (TUB) were analyzed by immunoblotting with specific antibodies. Experiments were quantified in Fiji and the EPS15 levels plotted as fold-increase compared to controls. Data are mean ± SEM, N = 4. *p≤0.05. Note that SPOPL depletion does not further increase the levels of both EPS15 mutants. (E) Total cell extracts were prepared from HeLa cells expressing either GFP-tagged wild-type, the EPS15S744-S746A mutant or the EPS15K793R mutant in the presence (+) or absence (-) of HA-tagged SPOPL overexpression. The levels of EPS15-GFP, SPOPL-HA and control GAPDH were analyzed by immunoblotting. Note that overexpression of SPOPL-HA is able to induce degradation of wild-type but not the EPS15S744-S746A-GFP or the EPS15K793R-GFP mutant.DOI://dx.doi.org/10.7554/eLife.13841.009EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(A) Alignments of the carboxy-terminal domains of EPS15 proteins from various species. Conserved SPOPL-binding motifs and putative ubiquitination sites are highlighted by yellow boxes. (B) Peptides containing EPS15 modification sites were quantified with LC-MS/MS after enrichment of the K-epsilon -GG motif from whole cell HeLa digests treated with siSPOPL and siControl. Raw intensities for each of the triplicate LC-MS/MS runs are shown with each of the siControl conditions scaled to 100% intensity. Normalized precursor mass intensity profiles for EPS15 sites corresponding to K793, K801 and K693 are shown, with only K793 showing significant downregulation in the depletion condition. Quantification of a peptide corresponding to beta -Actin K113 and the poly-ubiquitin K11 linkage peptide is also shown to demonstrate that enrichment variations did not influence the quantification of the EPS15 sites. Additionally, the total ion chromatographic intensities for each run are plotted to provide insight into the consistency of each of the separate experiments performed on different days. Data are mean ± SD, N = 3. (C) HeLa cell lines stably expressing wild-type EPS15-GFP, the EPS15S744-746A-GFP mutant or the EPS15K793R-GFP mutant from the inducible doxycycline-promoter were treated with doxycycline for 3 days, and analyzed by live cell imaging. Displayed are maximal projections of Z-stack acquisitions, fully covering cell height. Scale bar = 10 μm.DOI://dx.doi.org/10.7554/eLife.13841.010 Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-127_mouse-monoclonal-aip-ara9-antibody-35-2-23220248215548.jpg)
![Immunohistochemistry: AIP/ARA9 Antibody (35-2) [NB100-127] - Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A & B—Examples of low AIP expression; C & D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody (negative control); F—Normal human pituitary staining with AIP (positive control); Scale bar = 1000 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-127_mouse-monoclonal-aip-ara9-antibody-35-2-310202415284525.jpg)
![Immunohistochemistry: AIP/ARA9 Antibody (35-2) [NB100-127] - Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A & B—Examples of low AIP expression; C & D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody (negative control); F—Normal human pituitary staining with AIP (positive control); Scale bar = 1000 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-127_mouse-monoclonal-aip-ara9-antibody-35-2-310202415175264.jpg)
![Immunohistochemistry: AIP/ARA9 Antibody (35-2) [NB100-127] - Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A & B—Examples of low AIP expression; C & D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody (negative control); F—Normal human pituitary staining with AIP (positive control); Scale bar = 1000 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-127_mouse-monoclonal-aip-ara9-antibody-35-2-310202415345336.jpg)
![Immunohistochemistry: AIP/ARA9 Antibody (35-2) [NB100-127] - Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A & B—Examples of low AIP expression; C & D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody (negative control); F—Normal human pituitary staining with AIP (positive control); Scale bar = 1000 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-127_mouse-monoclonal-aip-ara9-antibody-35-2-310202415345334.jpg)
![Immunohistochemistry: AIP/ARA9 Antibody (35-2) [NB100-127] - Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A & B—Examples of low AIP expression; C & D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody (negative control); F—Normal human pituitary staining with AIP (positive control); Scale bar = 1000 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-127_mouse-monoclonal-aip-ara9-antibody-35-2-310202415345332.jpg)
![Immunohistochemistry: AIP/ARA9 Antibody (35-2) [NB100-127] - Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A & B—Examples of low AIP expression; C & D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody (negative control); F—Normal human pituitary staining with AIP (positive control); Scale bar = 1000 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-127_mouse-monoclonal-aip-ara9-antibody-35-2-310202415304289.jpg)