![Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on RH30 cells with O-GlcNAc [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Alexa-Fluor-R-647-Flow-Cytometry-NB300-524AF647-img0005.jpg "Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on RH30 cells with O-GlcNAc [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Dr, Fi, Ha, Pm, V-Vi, XpSpecies Glossary |
| Applications | WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IP, ChIP |
| Clone | RL2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alexa Fluor 647 |
| Immunogen | Pore complex-lamina fraction purified from rat liver nuclear envelopes. |
| Localization | Cytoplasmic and Nuclear |
| Specificity | Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
Flow | Mouse | 10/05/2015 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for O-GlcNAc Antibody (NB300-524AF647)Find related products by research area.
|
|
O-GlcNAc, Glucose Deprivation and Cancer O-linked beta-N-acetylglucosamine (O-GlcNAc) is a sugar attachment to serine or threonine hydroxyl moieties on nuclear and cytoplasmic proteins. O-GlcNAc modified proteins are generally either cytoplasmic or nuclear proteins, and unlike asparagine-lin... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor (R) 647] [NB300-524AF647] - An intracellular stain was performed on Jurkat cells with O-GlcNAc antibody (RL2) NB300-524 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Alexa-Fluor-R-647-Flow-Cytometry-NB300-524AF647-img0001.jpg "Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor (R) 647] [NB300-524AF647] - An intracellular stain was performed on Jurkat cells with O-GlcNAc antibody (RL2) NB300-524 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on SK-MEL-28 cells with O-GlcNAc antibody (RL2) NB300-524AF647 (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature . Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Alexa-Fluor-R-647-Flow-Cytometry-NB300-524AF647-img0002.jpg "Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on SK-MEL-28 cells with O-GlcNAc antibody (RL2) NB300-524AF647 (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature . Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on HeLa cells with O-GlcNAc Antibody [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Alexa-Fluor-R-647-Flow-Cytometry-NB300-524AF647-img0003.jpg "Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on HeLa cells with O-GlcNAc Antibody [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on Neuro2a cells with O-GlcNAc Antibody [RL2] NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Alexa-Fluor-R-647-Flow-Cytometry-NB300-524AF647-img0004.jpg "Flow Cytometry: O-GlcNAc Antibody (RL2) [Alexa Fluor® 647] [NB300-524AF647] - An intracellular stain was performed on Neuro2a cells with O-GlcNAc Antibody [RL2] NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
