O-GlcNAc Antibody (RL2) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Dr, Fi, Ha, Pm, Vi, XpSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
RL2
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594

Order Details

O-GlcNAc Antibody (RL2) [Alexa Fluor® 594] Summary

Immunogen
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Localization
Cytoplasmic and Nuclear
Specificity
Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 26004176). Xenopus reactivity reported in scientific literature (PMID: 17329255). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for O-GlcNAc Antibody (RL2) [Alexa Fluor® 594]

  • GlcNAc
  • O-linked N-acetylglucosamine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for O-GlcNAc Antibody (NB300-524AF594) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-524AF350
Alexa Fluor 405 NB300-524AF405
Alexa Fluor 488 NB300-524AF488
Alexa Fluor 532 NB300-524AF532
Alexa Fluor 594 NB300-524AF594
Alexa Fluor 647 NB300-524AF647
Alexa Fluor 700 NB300-524AF700
Alexa Fluor 750 NB300-524AF750
Allophycocyanin NB300-524APC
Allophycocyanin/Cy7 NB300-524APCCY7
Biotin NB300-524B
DyLight 350 NB300-524UV
DyLight 405 NB300-524V
DyLight 488 NB300-524G
DyLight 550 NB300-524R
DyLight 594 NB300-524DL594
DyLight 650 NB300-524C
DyLight 680 NB300-524FR
DyLight 755 NB300-524IR
HRP NB300-524H
Janelia Fluor 549 NB300-524JF549
Janelia Fluor 646 NB300-524JF646
PE NB300-524PE
PE/Atto594 NB300-524PEATT594
PE/Cy5.5 NB300-524PECY55
PE/Cy7 NB300-524PECY7
PerCP NB300-524PCP

Additional O-GlcNAc Products

Array NB300-524AF594

Blogs on O-GlcNAc.

O-GlcNAc, Glucose Deprivation and Cancer
O-linked beta-N-acetylglucosamine (O-GlcNAc) is a sugar attachment to serine or threonine hydroxyl moieties on nuclear and cytoplasmic proteins. O-GlcNAc modified proteins are generally either cytoplasmic or nuclear proteins, and unlike asparagine-lin...  Read full blog post.

Recombinant Monoclonal Antibodies

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