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ATG9A Antibody [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Dr, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-56893AF350
Alexa Fluor 405
NB110-56893AF405
Alexa Fluor 488
NB110-56893AF488
Alexa Fluor 532
NB110-56893AF532
Alexa Fluor 594
NB110-56893AF594
Alexa Fluor 647
NB110-56893AF647
Alexa Fluor 700
NB110-56893AF700
Alexa Fluor 750
NB110-56893AF750
Biotin
NB110-56893B
DyLight 350
NB110-56893UV
DyLight 405
NB110-56893V
DyLight 488
NB110-56893G
DyLight 550
NB110-56893R
DyLight 594
NB110-56893DL594
DyLight 650
NB110-56893C
DyLight 680
NB110-56893FR
DyLight 755
NB110-56893IR
FITC
NB110-56893F
HRP
NB110-56893H
Janelia Fluor 646
NB110-56893JF646
Unconjugated
NB110-56893
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ATG9A Antibody [Janelia Fluor® 549] Summary

Immunogen
A synthetic peptide made to a region within the C-terminus of human ATG9A (between residues 750-839). [Swiss-Prot: Q7Z3C6].
Localization
Membrane; multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG9A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 26353861).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for ATG9A Antibody [Janelia Fluor® 549]

  • APG9 autophagy 9-like 1 (S. cerevisiae)
  • APG9 autophagy 9-like 1
  • APG9L1APG9-like 1
  • ATG9 autophagy related 9 homolog A (S. cerevisiae)
  • autophagy 9-like 1 protein
  • autophagy-related protein 9A
  • FLJ22169
  • mATG9
  • MGD3208

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TBK1 Antibody
(108A429)
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Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.
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Immunohistochemistry-Paraffin: WIPI2 Antibody [NBP1-88879] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
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ATG16L1 Antibody
NB110-60928
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Immunohistochemistry-Paraffin: ATG16L1 Antibody [NB110-60928] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.
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FAK Antibody
(SR46-04)
NBP2-67327
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NBP1-84267
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STING/TMEM173
Antibody
NBP2-24683
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RB1CC1 Antibody
NB100-77279
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Western Blot: RB1CC1 Antibody [NB100-77279] - Detection of Human FIP200/RB1CC1 on HeLa whole cell lysate using NB100-77279. FIP200/RB1CC1 was also immunoprecipitated by rabbit anti-FIP200/RB1CC1 antibody NB100-77317.
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ATG9A Antibody
[Janelia Fluor® ...
NB110-56893JF549
ATG9A Antibody [Janelia Fluor® 549]
Species: Hu, Mu, Rt, Bv, Ch, Dr, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

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FAQs for ATG9A Antibody (NB110-56893JF549) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-56893AF350
Alexa Fluor 405 NB110-56893AF405
Alexa Fluor 488 NB110-56893AF488
Alexa Fluor 532 NB110-56893AF532
Alexa Fluor 594 NB110-56893AF594
Alexa Fluor 647 NB110-56893AF647
Alexa Fluor 700 NB110-56893AF700
Alexa Fluor 750 NB110-56893AF750
Biotin NB110-56893B
DyLight 350 NB110-56893UV
DyLight 405 NB110-56893V
DyLight 488 NB110-56893G
DyLight 550 NB110-56893R
DyLight 594 NB110-56893DL594
DyLight 650 NB110-56893C
DyLight 680 NB110-56893FR
DyLight 755 NB110-56893IR
FITC NB110-56893F
HRP NB110-56893H
Janelia Fluor 549 NB110-56893JF549
Janelia Fluor 646 NB110-56893JF646
Learn about Custom Antibody Labeling

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Bioinformatics Tool for ATG9A Antibody (NB110-56893JF549)

Discover related pathways, diseases and genes to ATG9A Antibody (NB110-56893JF549). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for ATG9A Antibody (NB110-56893JF549)

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Pathways for ATG9A Antibody (NB110-56893JF549)

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PTMs for ATG9A Antibody (NB110-56893JF549)

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Research Areas for ATG9A Antibody (NB110-56893JF549)

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Blogs on ATG9A.

Check out the latest blog posts on ATG9A.
Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.
Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy
By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat...  Read full blog post.

  Read full blog post.
Atg9b - a marker for autophagosome induction and assembly
Atg9 is the only essential transmembrane protein involved in cellular autophagy. Autophagy regulates cellular homeostasis by allowing the turnover and recycling of misfolded proteins and damaged organelles. Formation of the double-membrane isolatio...  Read full blog post.
ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.
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Western Blot: LC3A Antibody [NB100-2331] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (http://stemcellres.com/content/5/6/140), licensed under a CC-BY licence.
LC3A Antibody
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Western Blot: ATG5 Antibody [NB110-53818] - Western blot analysis in mouse wildtype ES cell lysate (Lane 1) using NB110-53818. Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.
ATG5 Antibody
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Bioinformatics

Gene Symbol ATG9A