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Product Search for Immunoprecipitation

Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.

Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:

13175 Results for "Immunoprecipitation"

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NB100-127

AIP/ARA9 Antibody (35-2) - BSA Free

(4 Reviews)

(33 Publications)

0.1 ml

NB100-127

Western Blot: AIP/ARA9 Antibody (35-2) [NB100-127] - Detection on HEK 293T/17 cells. Lane 1: IP ARA9, WB ARA9, Lane 2: WB ARA9 with whole cell lysate. Photo courtesy of Liling Zeng, Boston University.

<b>Biological Strategies Validation. </b>Western Blot: AIP/ARA9 Antibody (35-2) [NB100-127] - Effect of miR-34a (A) and anti-miR-34a (B) on endogenous AIP protein levels in HEK293 and in GH3 (C) cells 48 hours after transfection. Data are shown as mean +/- SEM, **, P<0.01 ***, P<0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25658813/) licensed under a CC-BY license.

Flow Cytometry: AIP/ARA9 Antibody (35-2) [NB100-127] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using AIP/ARA9 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Immunohistochemistry-Paraffin: AIP/ARA9 Antibody (35-2) [NB100-127] - AIP/ARA9 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human AIP/ARA9 (35-2) Monoclonal Antibody (Catalog # NB100-127) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in splenocytes.

EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(A) Cartoon of human EPS15 domain-organization and the amino-acid sequence. Indicated by color code are the SPOP/SPOPL binding site (red) and the lysine residue (yellow), which is ubiquitinated in a CRL3SPOPL–dependent manner in vivo. In addition, the amino-terminal Ca2+-binding EF-hand motifs (EH), the coiled-coil domain involved in dimerization and the two carboxy-terminal ubiquitin-interacting motifs (UIMs) involved in ubiquitin-binding are indicated. (B) EPS15 ubiquitin-profiling. Peptides containing EPS15 modification sites were quantified with LC-MS/MS after enrichment of the K-epsilon -GG motif from whole cell digests of HeLa cells treated with siSPOPL or siControl. Normalized precursor mass intensity profiles for EPS15 sites corresponding to K793, K801 and K693 are shown (raw data in Figure 4—figure supplement 1B). Quantification of the beta -Actin K113 and the polyubiquitin K11 linkage peptide control for comparable enrichment. Data are mean ± SD, N = 3. **p≤0.01. (C) Purified SPOPL was incubated as indicated with GST-tagged wild-type EPS15 or GST-EPS15 mutants, where the predicted SPOPL binding motifs have been mutated individually (GST-EPS15S605-S607A and EPS15S744-S746A, respectively), pulled down with glutathione sepharose (IP [GST]) and bound proteins were analyzed by Coomassie blue staining (upper panel) and immunoblotting (lower panels). Note that SPOPL readily binds to GST-EPS15 and GST-EPS15S605-S607A, but this interaction is strongly reduced with the GST-EPS15S744-S746A mutant. (D) HeLa cells stably expressing GFP-tagged wild-type EPS15, the EPS15S744-S746A or the EPS15K793R mutants from a doxycycline-inducible promoter were transfected as indicated (+) with control siRNA or siRNA depleting SPOPL. The levels of EPS15-GFP, EGFR and for control tubulin (TUB) were analyzed by immunoblotting with specific antibodies. Experiments were quantified in Fiji and the EPS15 levels plotted as fold-increase compared to controls. Data are mean ± SEM, N = 4. *p≤0.05. Note that SPOPL depletion does not further increase the levels of both EPS15 mutants. (E) Total cell extracts were prepared from HeLa cells expressing either GFP-tagged wild-type, the EPS15S744-S746A mutant or the EPS15K793R mutant in the presence (+) or absence (-) of HA-tagged SPOPL overexpression. The levels of EPS15-GFP, SPOPL-HA and control GAPDH were analyzed by immunoblotting. Note that overexpression of SPOPL-HA is able to induce degradation of wild-type but not the EPS15S744-S746A-GFP or the EPS15K793R-GFP mutant.DOI://dx.doi.org/10.7554/eLife.13841.009EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(A) Alignments of the carboxy-terminal domains of EPS15 proteins from various species. Conserved SPOPL-binding motifs and putative ubiquitination sites are highlighted by yellow boxes. (B) Peptides containing EPS15 modification sites were quantified with LC-MS/MS after enrichment of the K-epsilon -GG motif from whole cell HeLa digests treated with siSPOPL and siControl. Raw intensities for each of the triplicate LC-MS/MS runs are shown with each of the siControl conditions scaled to 100% intensity. Normalized precursor mass intensity profiles for EPS15 sites corresponding to K793, K801 and K693 are shown, with only K793 showing significant downregulation in the depletion condition. Quantification of a peptide corresponding to beta -Actin K113 and the poly-ubiquitin K11 linkage peptide is also shown to demonstrate that enrichment variations did not influence the quantification of the EPS15 sites. Additionally, the total ion chromatographic intensities for each run are plotted to provide insight into the consistency of each of the separate experiments performed on different days. Data are mean ± SD, N = 3. (C) HeLa cell lines stably expressing wild-type EPS15-GFP, the EPS15S744-746A-GFP mutant or the EPS15K793R-GFP mutant from the inducible doxycycline-promoter were treated with doxycycline for 3 days, and analyzed by live cell imaging. Displayed are maximal projections of Z-stack acquisitions, fully covering cell height. Scale bar = 10 μm.DOI://dx.doi.org/10.7554/eLife.13841.010 Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: AIP/ARA9 Antibody (35-2) [NB100-127] - Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A & B—Examples of low AIP expression; C & D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody (negative control); F—Normal human pituitary staining with AIP (positive control); Scale bar = 1000 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-127SS

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MAB901

MMP-1 Antibody (36665) [Unconjugated]

(42 Publications)

200 μg

MAB901

Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of PC-3 human prostate cancer parental cell line and MMP-1 knockout PC-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Inhibition of PKC alpha decreases the protein expression of MMP‐1 and MMP‐9 induced by cell cross‐talk in SMC. A, Evaluation of PKC alpha activation in control SMC (S NG) vs SMC previously co‐cultured with MAC in HG (Sc HG). Note that, the phosphorylated form of PKC alpha is significantly increased in SMC that were before exposed to MAC in HG conditions. Silencing of CCR2 or p65 decreases the phosphorylation of PKC alpha to control levels. B, Effect of PKC alpha inhibitor – Go 6976 on the MMP‐1 and MMP‐9 protein expression induced by SMC‐MAC cross‐talk. S NG – control SMC, Sc NG – SMC which were co‐cultured with MAC in normal glucose, Sc HG ‐ SMC which were co‐cultured with MAC in high glucose, Sc HGGo ‐ SMC which were co‐cultured with MAC in high glucose in presence of 1 μmol/L Go 6976. n = 3, *P < .05, **P < .01, #P < .05, ##P < .01 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29992758), licensed under a CC-BY license. Not internally tested by R&D Systems.

DDC up-regulates MMP-1 through ERK1/2 and Akt activation(A) Co-cultures were treated with 100 μM DDC for the indicated time. Phosphorylation of ERK1/2, p38 and Akt were determined by Western blotting analysis. The corresponding non-phosphorylated ERK1/2, p38, Akt and beta -actin were used for protein loading control. (B) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of ERK1/2 inhibitor (U0126, 10 μM). MMP-1 protein levels were analysed by Western blotting. (C) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of p38 inhibitor (SB203580, 10 μM). MMP-1 protein levels were analysed by Western blotting. (D) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of Akt inhibitor (T3830, 50 μM). MMP-1 protein levels were analysed by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

DDC up-regulates MMP-1 expression in a dose-dependent manner and lowered collagen I in LX-2 cellsCo-culture systems were treated with 25, 50, 100 and 200 μM DDC for 24 h. LX-2 cells and the culture medium were collected and analysed. (A) Quantitative RT-PCR results of MMP-1 expression in LX-2 cells. The results were normalized with beta -actin and expressed as fold increase relative to the control in each experiment. (B) 20 μg aliquots of total protein extracts from LX-2 cells were subjected to Western blotting analysis with anti-MMP-1 antibody (MAB 901 and MAB 3223 for active form only) and beta -actin antibody as a loading control, as described in the Materials and Methods section. (C) 30 μg of LX-2 lysates or 20 μl aliquots of 10-fold concentrated culture medium with 5×loading buffer were assayed by Casein Zymography for MMP-1 activity. (D) Levels of collagen I in LX-2 cells were assayed by western blotting. (E) Levels of collagen I in the culture medium were assayed by ELISA. *P<0.05 compared with untreated C3A-2E1/LX-2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Co‐culture of SMC and MAC in high glucose conditions (24 h) induces a significant increase in the protein expression of MMP‐1 and MMP‐9. A, B: Protein expression of MMP‐1 (A) and MMP‐9 (B) was assessed in SMC after co‐culture with THP‐derived macrophages and evaluated by Western blot assay. C, D: Protein expression of MMP‐1 (C) and MMP‐9 (D) in SMC after co‐culture with human macrophages derived from freshly isolated monocytes from control subjects (CM) or diabetic patients with ACS (DM). S NG –control SMC, S HG – SMC exposed to HG, Sc NG or Sc CM – SMC which were co‐cultured with MAC in normal glucose, Sc HG or Sc DM ‐ SMC which were co‐cultured with MAC in high glucose. n = 3, *P < .05, **P < .01, ***P < .001, #P < .05, ##P < .01, ###P < .001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29992758), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of CCR2 and p65 silencing in the regulation of MMP‐1 and MMP‐9 expression in SMC. Protein expression of MMP‐1 (A) and MMP‐9 (B) determined by Western blot in control SMC (S NG), SMC which were co‐cultured with MAC in HG conditions (Sc HG), and SMC subjected to silencing of CCR‐2 and p65 and co‐cultured with MAC in HG conditions (Cneg, p65, CCR2 siRNA). Note that silencing of CCR2 or p65 decreases significantly the protein expression of MMP‐1 and MMP‐9 induced by SMC‐MAC co‐culture in high glucose conditions. n = 3, *P < .05, #P < .05 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29992758), licensed under a CC-BY license. Not internally tested by R&D Systems.

DDC up-regulates MMP-1 expression in an H2O2-associated mannerCo-cultures were treated with 100 μM DDC, 2000 Units catalase, 25 μM vitamin E and 5μM H2O2 combined with 100 μM DDC for 24 h. Cell lysates from the LX-2 cells were collected and analysed. (A) 20 μg aliquots of total protein extracts from LX-2 cells were subjected to Western blotting analysis with anti-MMP-1 antibody (MAB 901) and beta -actin antibody as a loading control, as described in the Materials and Methods section. (B) Levels of collagen I in LX-2 cells were assayed by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

DDC up-regulates MMP-1 expression in LX-2 cellsCo-culture systems were treated with 100 μM DDC for 24 h. LX-2 cells and the culture medium were collected and analysed. (A) Quantitative RT-PCR results of MMP-1 expression in LX-2 cells. The results were normalized with beta -actin and expressed as fold increase relative to the control in each experiment. (B) 20 μg aliquots of total protein extracts from LX-2 cells were subjected to western blotting analysis with anti-MMP-1 antibody (MAB 901) and beta -actin antibody as a loading control, as described in the Materials and Methods section. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

MMP-1, MMP-3, and MMP-13 expression. (A-D) MMP-1; (E-H) MMP-3; (I-L) MMP-13 (A,E,I) ACL from young normal knee; (B,F,J) ACL from aging knee; (C,G,K) fibroblast-like cell aggregates in the degenerated ACL; (D,H,L) chondrocyte-like cell aggregates in the degenerated ACL. (Original magnification ×40). The graph (M) represents the percentage (mean ± SEM) of MMP-1-, MMP-3-, and MMP-13-positive cells in each group. **P < 0.01. (N) Western blotting for MMP-1, MMP-3, and MMP-13 (n = 3 for each category). Image collected and cropped by CiteAb from the following open publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar4165), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB901-SP

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NB300-202

STEP Antibody (23E5) - BSA Free

(1 Review)

(41 Publications)

0.1 ml

NB300-202

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-202AF350
Alexa Fluor 405	NB300-202AF405
Alexa Fluor 488	NB300-202AF488
Alexa Fluor 532	NB300-202AF532
Alexa Fluor 594	NB300-202AF594
Alexa Fluor 647	NB300-202AF647
Alexa Fluor 700	NB300-202AF700
Alexa Fluor 750	NB300-202AF750
Allophycocyanin	NB300-202APC
Biotin	NB300-202B
CoraFluor 1	NB300-202CL1
DyLight 350	NB300-202UV
DyLight 405	NB300-202V
DyLight 488	NB300-202G
DyLight 550	NB300-202R
DyLight 594	NB300-202DL594
DyLight 650	NB300-202C
DyLight 680	NB300-202FR
DyLight 755	NB300-202IR
FITC	NB300-202F
HRP	NB300-202H
Janelia Fluor 525	NB300-202JF525
Janelia Fluor 549	NB300-202JF549
Janelia Fluor 646	NB300-202JF646
Janelia Fluor 669	NB300-202JF669
PE	NB300-202PE
PerCP	NB300-202PCP
mFluor Violet 450 SE	NB300-202MFV450
mFluor Violet 500 SE	NB300-202MFV500
mFluor Violet 610 SE	NB300-202MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-202SS

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NBP1-77080

NLRP3/NALP3 Antibody - BSA Free

(2 Reviews)

(35 Publications)

0.1 mg

NBP1-77080

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. Representative image of immunofluorescent staining of NLRP3 related genes in corneal and conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32884023/) licensed under a CC-BY license.

hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. (A) Representative image of immunofluorescent staining of NLRP3 related genes in corneal and conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). (B,C) qRT-PCR showed the mRNA levels of NLRP3 related genes in conjunctiva and cornea (four corneas/conjunctivas were mixed as a sample). Data was shown as mean ± SEM. *p < 0.05.

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. (A) Representative image of immunofluorescent staining of NLRP3 related genes in corneal & conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). (B,C) qRT-PCR showed the mRNA levels of NLRP3 related genes in conjunctiva & cornea (four corneas/conjunctivas were mixed as a sample). Data was shown as mean ± SEM. *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32884023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Co-immunoprecipitation assay: Figure showing interaction of NLRP3, NLRC4 & NAIP with ASC during CFT073 infection in THP-1m. Cytoplasmic extracts of THP-1m infected with or without CFT073, 6 h post stimulation were subjected to IP & WB by various antibodies (ASC, NLRP3, NLRC4, & NAIP) as described in material & methods. Cytoplasmic extract used as input was subjected to check NLRP3, ASC, NLRC4 & NAIP expression as shown in figure. ASC antibody was used to co-immunoprecipitate the complex from cytoplasmic extracts & then immunoblotted to detect NLRP3, NLRC4 & NAIP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31551961), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 regulated the activation of NLRP3, rather than the activation of caspase-8, via NF-kappa B pathway. a, b NF-kappa B was activated in ischemic retina at the early stage after reperfusion. c, d Intraocular injection rHMGB1 or GA could promote or suppress the production of phosphor-NF-kappa B p65. e, f Intravitreal injection of NF-kappa B p65 inhibitor, JSH-23, significantly reduced the activation of NLRP3 & decreased the processing of IL-1 beta , g rather than caspase-8. However, intravitreal injection of caspase-8 inhibitor, Z-IETD-fmk, decreased the production of phosphor-NF-kappa B p65, obviously (h). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26224068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Ec

Species Glossary

Reactivity: Hu, Mu, Ec

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77080AF350
Alexa Fluor 405	NBP1-77080AF405
Alexa Fluor 488	NBP1-77080AF488
Alexa Fluor 532	NBP1-77080AF532
Alexa Fluor 594	NBP1-77080AF594
Alexa Fluor 647	NBP1-77080AF647
Alexa Fluor 700	NBP1-77080AF700
Alexa Fluor 750	NBP1-77080AF750
Allophycocyanin	NBP1-77080APC
Biotin	NBP1-77080B
CoraFluor 1	NBP1-77080CL1
DyLight 350	NBP1-77080UV
DyLight 405	NBP1-77080V
DyLight 488	NBP1-77080G
DyLight 550	NBP1-77080R
DyLight 594	NBP1-77080DL594
DyLight 650	NBP1-77080C
DyLight 680	NBP1-77080FR
DyLight 755	NBP1-77080IR
FITC	NBP1-77080F
HRP	NBP1-77080H
Janelia Fluor 525	NBP1-77080JF525
Janelia Fluor 549	NBP1-77080JF549
Janelia Fluor 646	NBP1-77080JF646
Janelia Fluor 669	NBP1-77080JF669
PE	NBP1-77080PE
PerCP	NBP1-77080PCP
mFluor Violet 450 SE	NBP1-77080MFV450
mFluor Violet 500 SE	NBP1-77080MFV500
mFluor Violet 610 SE	NBP1-77080MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77080SS

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AF2550

Osteoactivin/GPNMB Antibody [Unconjugated]

(40 Publications)

100 μg

AF2550

Western blot shows lysates of U-118-MG human glioblastoma/astrocytoma cell line and T98G human glioblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Osteoactivin/GPNMB at approximately 95 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Osteoactivin/GPNMB was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

<b>Genetic Strategies Validation. </b>U‑87 MG human glioblastoma/astrocytoma parental cell line ctrl and Osteoactivin/GPNMB U-87 MG KD cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of ctrl cells), red (antibody staining) and far-red (identification of KD cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. Ctrl and KD cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 0.2 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

<b>Genetic Strategies Validation. </b>Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma parental cell line and Osteoactivin/GPNMB knockdown U-87 MG cell line (KD). Nitrocellulose membrane was probed with 0.4 µg/mL Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) followed by HRP-conjugated secondary antibody. Specific bands were detected for Osteoactivin/GPNMB at approximately 76 and 120 kDa (as indicated) in the parental U-87 MG cell line, but is significantly reduced in the U-87 MG cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

$U‑87 MG human glioblastoma/astrocytoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Goat Anti-Human Osteoactivin/GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2550) pre-coupled to Dynabeads Protein G. Immunoprecipitated Osteoactivin/GPNMB was detected in Western Blot with a Rabbit Osteoactivin/GPNMB Antibody. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

GPNMB and galectin-3 levels are elevated in FTD-GRN brains. a, b GPNMB and galectin-3 levels (ng/mg protein) were measured in frontal lobe tissue lysates generated from cognitively normal controls (CTL; n = 27) and FTD-GRN patients (n = 25). Data analyzed using unpaired t-test. c Representative immunoblots for GPNMB and galectin-3 in frontal lobe lysates from cognitively normal controls (n = 8) and FTD-GRN (n = 8) patients. d GPNMB levels (ng/mL) in CSF samples form cognitively normal controls (n = 14), FTD-GRN (n = 9), FTD-C9orf72 (n = 12) and FTD-MAPT (n = 12) samples quantified by ELISA. Data analyzed using one-way ANOVA. e, f GPNMB immunostaining was performed on frontal lobe tissue sections from cognitively normal controls (n = 5) (e) and FTD-GRN (n = 5) (f) patients. g, h Immunostaining for p-TDP 43 was stained on adjacent sections from identical samples in e, f as marker of FTLD pathology. i GPNMB staining intensity in human brain sections (e, f) were measured and presented as fold change. Representative immunofluorescence staining for cell markers (green) (j, n, r), GPNMB (red) (k, o, s), DAPI (blue) (i, p, t) in paraffin sections of brains from FTD-GRN cases. Iba-1, GFAP, NeuN used for markers of human microglia, astrocytes, and neurons respectively. GPNMB and Iba-1 signals overlap (arrow) (m) whereas, no overlapping signal was observed in co-staining with GFAP or NeuN (q, u). Scale bars were labeled in the images. Data analyzed by unpaired t-test. Scale bars (20 µm) labeled in images and quantitative data are shown as mean ± SEM, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33028409), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2550

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2550-SP

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AF2910

Alkaline Phosphatase/ALPL Antibody [Unconjugated]

(44 Publications)

100 μg

AF2910

Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 2 µg/mL overnight at 4 °C. Tissue was stained red and counterstained with DAPI (blue). Image courtesy of Paul J. Simmons, Ph.D., Director, Center for Stem Cell Research, The University of Texas Health Science Center at Houston, Houston, Texas, USA. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2910-SP

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NBP2-24873

Delta Np73 Antibody (38C674.2) - BSA Free

(41 Publications)

0.1 mg

NBP2-24873

Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) Staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry-Frozen: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) analysis of Delta Np73 in embryonic day 15 (A) and day 17 (B) E15 rat embryonic kidney at 1:2000. Delta Np73 is expressed in ureteric bud (UB) branches, and metanephrogenic mesenchyme (MM), as well as in cells invading the glomerular cleft. DeltaNp73 is expressed in the ureteric bud (UB) branches, metanephrogenic mesenchyme (MM), and nephron progenitors.

Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) Formalin-fixed, paraffin-embedded lung squamous cell carcinoma probed with p73 antibody at 5 ug/ml.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-313A

Alt Catalog# IMG-313A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24873AF350
Alexa Fluor 405	NBP2-24873AF405
Alexa Fluor 488	NBP2-24873AF488
Alexa Fluor 532	NBP2-24873AF532
Alexa Fluor 594	NBP2-24873AF594
Alexa Fluor 647	NBP2-24873AF647
Alexa Fluor 700	NBP2-24873AF700
Alexa Fluor 750	NBP2-24873AF750
Biotin	NBP2-24873B
DyLight 350	NBP2-24873UV
DyLight 405	NBP2-24873V
DyLight 488	NBP2-24873G
DyLight 550	NBP2-24873R
DyLight 594	NBP2-24873DL594
DyLight 650	NBP2-24873C
DyLight 680	NBP2-24873FR
DyLight 755	NBP2-24873IR
FITC	NBP2-24873F
HRP	NBP2-24873H
Janelia Fluor 549	NBP2-33262JF549
Janelia Fluor 646	NBP2-33262JF646

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24873SS
Azide and BSA Free	NBP2-33262

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NB600-362

HA Tag Antibody - BSA Free

(3 Reviews)

(42 Publications)

0.1 ml

NB600-362

Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-362] - HA-tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1 ug/mL rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals). Cells were stained using the appropriate secondary antibody, donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).

Western Blot: HA Tag Antibody - BSA Free [NB600-362] - 200, 67, 22, 7, or 2 ng of E. coli whole cell lysateexpressing a multi-tag fusion protein. Antibodies: Affinitypurified, goat anti-HA antibody used for WBat 0.04 ug/ml (1:25,000). Detection: Chemiluminescencewith an exposure time of 10 seconds.

Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-362] - HA tagged RFX6 overexpression in HEK293 stained with HA Tag antibody at a dilution of 1:250. ICC/IF image submitted by a verified customer review.

SUMO1 conjugates are not localized to amyloid plaques. (a) Sagittal brain sections of 24‐week‐old KI/AD & WT/AD animals were stained using anti‐HA antibodies (red), 6E10 antibodies (green) that label amyloid beta 1–42 among other amyloid beta variants (epitope lies within amino acids 3–8 of amyloid beta) & anti‐MAP2 antibodies (blue). Sections of the hippocampal subiculum are shown. Images are representatives of three independent experiments. Scale bar, 25 μm. (b) Anti‐HA signal intensity in amyloid plaques & in cell nuclei was quantified using ImageJ (N = 3, ***significance between WT/AD & KI/AD, p = .0007 in Student's t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29633471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$SUMO1 conjugates remain nuclear during increased amyloid burden. (a) Western blot analysis of subcellular fractions of 36‐week‐old KI/WT & KI/AD mouse brain using anti‐HA antibody (upper two panels) & antibodies to GluN1 (a marker of the postsynaptic compartment) & Synaptophysin (a marker of the presynaptic compartment) to validate the fractionation procedure (lower two panels). H, homogenate; P1, nuclear pellet; S1, supernatant after P1 sedimentation; P2, crude synaptosomal pellet; S2, supernatant after P2 sedimentation; LP1, lysed synaptosomal membranes; LS1, supernatant after LP1 sedimentation; LP2, pellet after LS1 sedimentation, SPM, synaptic plasma membranes. Bracket indicates the anti‐HA signal representing SUMOylation, arrow indicates RanGAP1, stars indicate nonspecific signal detected by the anti‐HA antibody. (b) Brain sagittal sections of aged (36 weeks old) KI/AD (left panels) & WT/AD (right panels) mice were immunostained using antibodies directed against HA (red, labels HA‐HA conjugates), MAP2 (green, labels neuronal somata & dendrites), & Synapsin1 (Syn1, magenta, labels synapses). The images show triple‐labeled neurons of hippocampal subiculum (b), cortical layer 5 (c), & proximal apical dendrite from hippocampal CA3 (d). The white line shows the orientation of the line‐scan used to generate the image stack shown in the bottom side view. Scale bar: 10 μm. Note that the anti‐HA immunosignal is mainly located in neuronal nuclei, only background staining is observed in WT/AD mice. Little anti‐HA signal is observed along MAP2‐positive structures & does not colocalize with Synapsin1 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29633471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Species Glossary

Reactivity: ET

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-362AF350
Alexa Fluor 405	NB600-362AF405
Alexa Fluor 488	NB600-362AF488
Alexa Fluor 532	NB600-362AF532
Alexa Fluor 594	NB600-362AF594
Alexa Fluor 647	NB600-362AF647
Alexa Fluor 700	NB600-362AF700
Alexa Fluor 750	NB600-362AF750
Allophycocyanin	NB600-362APC
Biotin	NB600-362B
CoraFluor 1	NB600-362CL1
DyLight 350	NB600-362UV
DyLight 405	NB600-362V
DyLight 488	NB600-362G
DyLight 550	NB600-362R
DyLight 594	NB600-362DL594
DyLight 650	NB600-362C
DyLight 680	NB600-362FR
DyLight 755	NB600-362IR
FITC	NB600-362F
HRP	NB600-362H
Janelia Fluor 525	NB600-362JF525
Janelia Fluor 549	NB600-362JF549
Janelia Fluor 646	NB600-362JF646
Janelia Fluor 669	NB600-362JF669
PE	NB600-362PE
PerCP	NB600-362PCP

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AF860

BID Antibody [Unconjugated]

(1 Review)

(40 Publications)

100 μg

AF860

Western blot shows Jurkat human acute T cell leukemia cell line treated with apoptosis inducer anti-Fas for the indicated times. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BID at approximately 20 kDa (as indicated). For additional reference short (5 seconds, left panel) and long (60 seconds, right panel) exposures are shown. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

$Survivin is predominantly expressed in obese hASCs and is influenced by the inflammatory environment. (a) Survivin protein expression and quantification in total adipose tissue (AT), adipocyte fraction (AD) and stromal-vascular fraction (SVF) from SAT of lean and obese patients. Fumarylacetoacetase (FAA) was used as a loading control. Ponceau S staining is also provided. *P<0.001 versus total AT and AD. (b) Survivin and p53 protein levels and quantification in hASCs from lean and obese patients. GAPDH was used as a loading control. *P<0.01 versus lean. (c) Survivin secreted levels and quantification in medium from lean- and obese-derived hASCs. Ponceau S staining was used to check loading. *P<0.05 versus lean conditioned medium. (d) Total cell extracts of lean- and obese-derived hASCs were subjected to immunoblotting with antibodies against apoptotic and antiapoptotic markers and GAPDH was used as a loading control. No significant differences were found. (e) Lean hASCs were cultured in RPMI medium (C: control) or for 24 h in conditioned medium (CM) from macrophages (M0, M1 and M2) and cells were subjected to immunobloting with survivin and GAPDH antibodies. *P<0.05 versus CM from control, and M0; #P<0.05 versus M2. (f) Left panel, lean hASCs were cultured in RPMI medium (C: control) or RPMI supplemented with IL-1 beta (IL1B) or IL-1 beta and IL-1 beta receptor antagonist (a); right panel, with CM of M1 macrophages or CM of M1 and IL-1 beta receptor antagonist (a). Survivin protein levels were measured and GAPDH was used as a loading control. Quantification of three experiments is shown. *P<0.05 versus control (c); #P<0.05 as indicated in figure. Data information: Values are expressed as mean±S.E.M. Statistical analysis: unpaired Student’s t-test. n=3–4 patients for each group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28518147), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$Survivin is predominantly expressed in obese hASCs and is influenced by the inflammatory environment. (a) Survivin protein expression and quantification in total adipose tissue (AT), adipocyte fraction (AD) and stromal-vascular fraction (SVF) from SAT of lean and obese patients. Fumarylacetoacetase (FAA) was used as a loading control. Ponceau S staining is also provided. *P<0.001 versus total AT and AD. (b) Survivin and p53 protein levels and quantification in hASCs from lean and obese patients. GAPDH was used as a loading control. *P<0.01 versus lean. (c) Survivin secreted levels and quantification in medium from lean- and obese-derived hASCs. Ponceau S staining was used to check loading. *P<0.05 versus lean conditioned medium. (d) Total cell extracts of lean- and obese-derived hASCs were subjected to immunoblotting with antibodies against apoptotic and antiapoptotic markers and GAPDH was used as a loading control. No significant differences were found. (e) Lean hASCs were cultured in RPMI medium (C: control) or for 24 h in conditioned medium (CM) from macrophages (M0, M1 and M2) and cells were subjected to immunobloting with survivin and GAPDH antibodies. *P<0.05 versus CM from control, and M0; #P<0.05 versus M2. (f) Left panel, lean hASCs were cultured in RPMI medium (C: control) or RPMI supplemented with IL-1 beta (IL1B) or IL-1 beta and IL-1 beta receptor antagonist (a); right panel, with CM of M1 macrophages or CM of M1 and IL-1 beta receptor antagonist (a). Survivin protein levels were measured and GAPDH was used as a loading control. Quantification of three experiments is shown. *P<0.05 versus control (c); #P<0.05 as indicated in figure. Data information: Values are expressed as mean±S.E.M. Statistical analysis: unpaired Student’s t-test. n=3–4 patients for each group Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28518147), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF860

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF860-SP

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NB100-900WB

HIF-1 alpha Antibody Pack

(36 Publications)

7 Vials

NB100-900WB

Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]

<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]

<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]

Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Analysis of HIF-1 alpha in overexpression and endogenous HIF-1 alpha & HIF-2 alpha using anti-HIF-1 alpha antibody NB100-134. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Wester blot image submitted by a verified customer review.

<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Western blot image submitted by a verified customer review. HIF-1 alpha Antibody [NB100-449]

Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein. HIF-1 alpha Antibody (HA111) [NB100-296]

Reactivity:

Hu, Mu, Rt, Ca, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse

Host: Mouse

Validated by:

Validated by:

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NB120-11427

Parvalbumin Antibody - BSA Free

(37 Publications)

0.1 mg

NB120-11427

Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin and parvalbumin antibodies shown in layer 2/3 at 63x magnification (scale = 10 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2013.00156/abstract), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human cerebellum tissue.

Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human skeletal muscle tissue.

Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human tonsil tissue.

Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin and parvalbumin antibodies shown in layer 2/3 at 63× magnification (scale = 10 μm).

Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin & parvalbumin antibodies shown in layer 2/3 at 63× magnification (scale = 10 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24065886), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Parvalbumin Antibody - BSA Free [NB120-11427] - Female Ambra1+/− mice show reduced numbers of hippocampal PV interneurons. a Nissl staining of dorsal hippocampus coronal sections from WT & Ambra1+/− female mice (scale bar: 500 μm). b Subdivision of hippocampal layers (stratum oriens, CA1 pyramidal layer, stratum radiatum, stratum lacunosum moleculare, stratum moleculare, dentate gyrus upper blade, hilus, dentate gyrus lower blade), corresponding to the area depicted in a (scale bar: 100 μm). Ambra1+/− females show normal hippocampal layering (n = 3 mice per genotype). c Double labeling for Ambra1 (green) & PV (red) in the CA1 from a WT female; sections were DAPI-counterstained (blue) for neuronal nuclei (scale bar: 50 μm). The higher magnification image (scale bar, 5 μm) from the stratum pyramidale shows a PV neuron (right; merge in orange) & an unidentified neuron (left), both expressing Ambra1. The DAPI signal is omitted for clarity & neuron borders are indicated by a dash line (n = 3 mice; three sections per animal). d, e Immunohistochemical labeling of PV in a coronal brain section of dorsal hippocampus obtained from female (d) & male (e) mice (scale bars: 500 μm). On the right are higher magnification images (scale bars: 50 μm). The plots show stereological quantification of PV interneurons (n = 6 mice per genotype & per gender; 14 sections per animal). The reduction in PV interneuron numbers is selective for female Ambra1+/− (two-tailed unpaired t test: *P = 0.043) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29488136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Fe

Species Glossary

Reactivity: Hu, Mu, Rt, Fe

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-11427AF350
Alexa Fluor 405	NB120-11427AF405
Alexa Fluor 488	NB120-11427AF488
Alexa Fluor 532	NB120-11427AF532
Alexa Fluor 594	NB120-11427AF594
Alexa Fluor 647	NB120-11427AF647
Alexa Fluor 700	NB120-11427AF700
Alexa Fluor 750	NB120-11427AF750
Allophycocyanin	NB120-11427APC
Biotin	NB120-11427B
CoraFluor 1	NB120-11427CL1
DyLight 350	NB120-11427UV
DyLight 405	NB120-11427V
DyLight 488	NB120-11427G
DyLight 550	NB120-11427R
DyLight 594	NB120-11427DL594
DyLight 650	NB120-11427C
DyLight 680	NB120-11427FR
DyLight 755	NB120-11427IR
FITC	NB120-11427F
HRP	NB120-11427H
Janelia Fluor 525	NB120-11427JF525
Janelia Fluor 549	NB120-11427JF549
Janelia Fluor 646	NB120-11427JF646
Janelia Fluor 669	NB120-11427JF669
PE	NB120-11427PE
PerCP	NB120-11427PCP
mFluor Violet 450 SE	NB120-11427MFV450
mFluor Violet 500 SE	NB120-11427MFV500
mFluor Violet 610 SE	NB120-11427MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB120-11427SS

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NB120-6405

MHC Class I Antibody (OX18) - BSA Free

(37 Publications)

0.1 mg

NB120-6405

Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).

Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-MHC Class I (OX18) NB120-6405 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow (Intracellular): MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were stained with MHC Class I NB120-6405 (Blue) and a matched isotype control NBP2-27287 (Orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by DyLight488-conjugated anti-mouse secondary antibody.

Effects of anodal tDCS on protein expression. Representative immunohistochemical images of Ox18 + cells in the SVZ ipsilateral to anodal tDCS or sham stimulation. Staining for Ox18 (MHC I) in the ipsilateral SVZ revealed more Ox18 + cells in animals treated by anodal tDCS (left panel) compared to sham stimulation (right panel) by trend (scale bars = 100 μm). Results are displayed as mean ± SEM.

Immunohistochemistry: MHC Class I Antibody (OX18) - BSA Free [NB120-6405] - Effects of anodal tDCS on protein expression. Representative immunohistochemical images of Ox18 + cells in the SVZ ipsilateral to anodal tDCS or sham stimulation. Staining for Ox18 (MHC I) in the ipsilateral SVZ revealed more Ox18 + cells in animals treated by anodal tDCS (left panel) compared to sham stimulation (right panel) by trend (scale bars = 100 μm). Results are displayed as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31708742), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Species Glossary

Reactivity: Rt

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-6405AF350
Alexa Fluor 405	NB120-6405AF405
Alexa Fluor 488	NB120-6405AF488
Alexa Fluor 532	NB120-6405AF532
Alexa Fluor 594	NB120-6405AF594
Alexa Fluor 647	NB120-6405AF647
Alexa Fluor 700	NB120-6405AF700
Alexa Fluor 750	NB120-6405AF750
Allophycocyanin	NB120-6405APC
Allophycocyanin/Cy7	NB120-6405APCCY7
Biotin	NB120-6405B
CoraFluor 1	NB120-6405CL1
DyLight 350	NB120-6405UV
DyLight 405	NB120-6405V
DyLight 488	NB120-6405G
DyLight 550	NB120-6405R
DyLight 594	NB120-6405DL594
DyLight 650	NB120-6405C
DyLight 680	NB120-6405FR
DyLight 755	NB120-6405IR
FITC	NB120-6405F
HRP	NB120-6405H
Janelia Fluor 525	NB120-6405JF525
Janelia Fluor 549	NB120-6405JF549
Janelia Fluor 646	NB120-6405JF646
Janelia Fluor 669	NB120-6405JF669
PE	NB120-6405PE
PE/Atto594	NB120-6405PEATT594
PE/Cy5.5	NB120-6405PECY55
PE/Cy7	NB120-6405PECY7
PerCP	NB120-6405PCP
mFluor Violet 450 SE	NB120-6405MFV450
mFluor Violet 500 SE	NB120-6405MFV500
mFluor Violet 610 SE	NB120-6405MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB120-6405SS
Azide and BSA Free	NBP2-80848

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

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