CD3 Antibody (17A2) [Alexa Fluor® 594]

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Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD3 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # ...read more
CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Alexa Fluor® 594‑conjugated Monoclonal Antibody (red; Catalog # FAB4841T) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF
Clone
17A2
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 594

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CD3 Antibody (17A2) [Alexa Fluor® 594] Summary

Immunogen
T cell hybridoma D1
Specificity
Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
Cd247
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 uL/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 Antibody (17A2) [Alexa Fluor® 594]

  • CD_antigen: CD3e
  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • IMD18
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma. This antibody has been shown to react with the epsilon chain of the CD3 complex. The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB4841U
Alexa Fluor 405 FAB4841V
Alexa Fluor 488 FAB4841G
Alexa Fluor 488 FAB4841G
Alexa Fluor 532 FAB4841X
Alexa Fluor 594 FAB4841T
Alexa Fluor 594 FAB4841T
Alexa Fluor 647 FAB4841R
Alexa Fluor 647 FAB4841R
Alexa Fluor 700 FAB4841N
Alexa Fluor 700 FAB4841N
Alexa Fluor 750 FAB4841S
Allophycocyanin FAB4841A
Allophycocyanin FAB4841A
Biotin BAM4841
DyLight 350 FAB4841D
DyLight 405 FAB4841E
DyLight 488 FAB4841K
DyLight 550 FAB4841L
DyLight 594 FAB4841M
DyLight 650 FAB4841W
DyLight 680 FAB4841Y
DyLight 755 FAB4841Z
Fluorescein FAB4841F
Fluorescein FAB4841F
HRP FAB4841H
Janelia Fluor 549 FAB4841I
Janelia Fluor 646 FAB4841J
PerCP (Peridinin-chlorophyll Protein Complex) FAB4841C
PerCP (Peridinin-chlorophyll Protein Complex) FAB4841C
Phycoerythrin FAB4841P
Phycoerythrin FAB4841P

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Bioinformatics

Gene Symbol Cd247