Flow Cytometry is a laser based biological assay used for cell detection, counting and sorting of specific biomarkers. Cells are first suspended in solution and fluorescently labeled antibodies are applied. Then the cells are passed single-file through a series of lasers which analyze size, shape and the fluorescent characteristics of each cell. Results can then be electronically gated to filter the data to a specific cell type and whether or not they contain bound antibodies. This form of assay is particularly useful for analyzing blood samples.
Novus offers a range of support options for flow cytometry when you buy a product to help you with your flow cytometry experiment including over 1,000 Flow images using our pre-conjugated antibodies:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF247-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-3638SS | |||
| Azide and BSA Free | NBP2-80568 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF941-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-425-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3437-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3870-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC202P | |||
| Carrier Free | MAB202-SP | |||
| Carrier Free | MAB202-500 | |||
| Carrier Free | IC202N-100UG | |||
| Carrier Free | IC202G-100UG | |||
| Carrier Free | IC202V-100UG | |||
| Carrier Free | IC202U-100UG | |||
| Carrier Free | IC202T-100UG | |||
| Carrier Free | IC202S-100UG | |||
| Carrier Free | IC202R-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40760SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77808SS | |||
| Azide and BSA Free | NBP2-80664 | |||
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs. Image collected and cropped by CiteAb from the following publication (eneuro.org/content/4/1/ENEURO.0171-16.2017), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Immunocytochemistry-Immunofluorescence-NB110-3638-img0005.jpg "Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs. Image collected and cropped by CiteAb from the following publication (eneuro.org/content/4/1/ENEURO.0171-16.2017), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Immunocytochemistry-Immunofluorescence-NB110-3638-img0003.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Western-Blot-NB110-3638-img0002.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic Induced Epitope Retrieval (PIER) was used.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Immunohistochemistry-Paraffin-NB110-3638-img0001.jpg)
![Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Detection of Alkaline Phosphatase/ALPP in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # NB110-3638, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Flow-Cytometry-NB110-3638-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - GRPs enhance axonal growth in DRGs in vitro. A–C, Representative montage images are shown for control dissociated DRG culture (A), dissociated DRGs cocultured with rat GRPs (B), & dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs (C). beta III-tubulin (red) immunofluorescence highlights the neurons; GRPs are visualized by immunostaining for alkaline phosphatase (green). D shows quantification for the average length of the longest axon per neuron ± SEM (n ≥ 30 neurons in three separate experiments; **p ≤ 0.01 by Student’s t test). Scale bar, 250 μm. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202415371956.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - GRPs enhance axonal growth in DRGs in vitro. A–C, Representative montage images are shown for control dissociated DRG culture (A), dissociated DRGs cocultured with rat GRPs (B), & dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs (C). beta III-tubulin (red) immunofluorescence highlights the neurons; GRPs are visualized by immunostaining for alkaline phosphatase (green). D shows quantification for the average length of the longest axon per neuron ± SEM (n ≥ 30 neurons in three separate experiments; **p ≤ 0.01 by Student’s t test). Scale bar, 250 μm. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202415175250.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - Coculture with neural & glial progenitor cells increases axonal outgrowth from adult DRG neurons. A–C, Representative montage images of control-dissociated DRG cultures (A), dissociated DRGs cocultured with rat GRPs/NRPs (B), & dissociated DRG cultures exposed to conditioned medium from parallel rat GRP/NRP cultures (C) are shown. Immunofluorescence for beta III-tubulin (red) & nestin (green) highlight DRG neurons & GRP/NRP nuclei, respectively. D, Quantitation of the average lengths of the longest axon per neuron (±SEM) for the above conditions is shown. Coculture with GRPs/NRPs significantly increases in axon length compared with the standard DRG culture; exposure to conditioned medium from GRP/NRP cultures showed a further increase in axon length (n ≥ 30 neurons in three separate experiments; *p ≤ 0.05 & ***p ≤ 0.001 by Student’s t test). Scale bar, 250 μm. E, Quantitation of axon growth parameters for 7 d injury-conditioned DRG neurons cultured on coverslips laid over a bed of GRP/NRP cells (coculture) or control conditions is shown (n ≥ 200 neurons analyzed in three separate experiments; p values represent ANOVA with Tukey post hoc analyses). Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202416235514.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - Coculture with neural & glial progenitor cells increases axonal outgrowth from adult DRG neurons. A–C, Representative montage images of control-dissociated DRG cultures (A), dissociated DRGs cocultured with rat GRPs/NRPs (B), & dissociated DRG cultures exposed to conditioned medium from parallel rat GRP/NRP cultures (C) are shown. Immunofluorescence for beta III-tubulin (red) & nestin (green) highlight DRG neurons & GRP/NRP nuclei, respectively. D, Quantitation of the average lengths of the longest axon per neuron (±SEM) for the above conditions is shown. Coculture with GRPs/NRPs significantly increases in axon length compared with the standard DRG culture; exposure to conditioned medium from GRP/NRP cultures showed a further increase in axon length (n ≥ 30 neurons in three separate experiments; *p ≤ 0.05 & ***p ≤ 0.001 by Student’s t test). Scale bar, 250 μm. E, Quantitation of axon growth parameters for 7 d injury-conditioned DRG neurons cultured on coverslips laid over a bed of GRP/NRP cells (coculture) or control conditions is shown (n ≥ 200 neurons analyzed in three separate experiments; p values represent ANOVA with Tukey post hoc analyses). Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202416284919.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - Coculture with neural & glial progenitor cells increases axonal outgrowth from adult DRG neurons. A–C, Representative montage images of control-dissociated DRG cultures (A), dissociated DRGs cocultured with rat GRPs/NRPs (B), & dissociated DRG cultures exposed to conditioned medium from parallel rat GRP/NRP cultures (C) are shown. Immunofluorescence for beta III-tubulin (red) & nestin (green) highlight DRG neurons & GRP/NRP nuclei, respectively. D, Quantitation of the average lengths of the longest axon per neuron (±SEM) for the above conditions is shown. Coculture with GRPs/NRPs significantly increases in axon length compared with the standard DRG culture; exposure to conditioned medium from GRP/NRP cultures showed a further increase in axon length (n ≥ 30 neurons in three separate experiments; *p ≤ 0.05 & ***p ≤ 0.001 by Student’s t test). Scale bar, 250 μm. E, Quantitation of axon growth parameters for 7 d injury-conditioned DRG neurons cultured on coverslips laid over a bed of GRP/NRP cells (coculture) or control conditions is shown (n ≥ 200 neurons analyzed in three separate experiments; p values represent ANOVA with Tukey post hoc analyses). Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-31020241628494.jpg)
![Western Blot: Perilipin Antibody [NB110-40760] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/mL anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Western-Blot-NB110-40760-img0002.jpg "Western Blot: Perilipin Antibody [NB110-40760] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/mL anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence.")
![Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NB110-40760-img0005.jpg)
![Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Flow-Cytometry-NB110-40760-img0008.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of fat tissue from mouse with Perilipin antibody at 1:200 dilution. The antibody generated an expected staining in the adipocytes towards periphery of the cells.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NB110-40760-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NB110-40760-img0006.jpg)
![Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on MCF7 cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Flow-Cytometry-NB110-40760-img0007.jpg)
![Imaging Mass Cytometry: Perilipin Antibody [NB110-40760] - Human bone marrow analyzed by IMC. Perilipin antibody conjugated with 151Eu and used 1:50 on human bone marrow FFPE. Perilipin - red. DNA- white. IMC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Imaging-Mass-Cytometry-NB110-40760-img0009.jpg)
![Flow Cytometry: Lightning-Link Fluorescein Antibody Labeling Kit [707-0010] - Mouse anti-duck CD4 antibody was conjugated with FITC using a Lightening-Link kit. The conjugated antibody was used to stain duck spleen and thymus followed by analysis with flow cytometry. Image from verified customer review.](http://images.novusbio.com/fullsize/Lightning-Link-Fluorescein-Antibody-Labeling-Kit-Flow-Cytometry-707-0010-img0003.jpg "Flow Cytometry: Lightning-Link Fluorescein Antibody Labeling Kit [707-0010] - Mouse anti-duck CD4 antibody was conjugated with FITC using a Lightening-Link kit. The conjugated antibody was used to stain duck spleen and thymus followed by analysis with flow cytometry. Image from verified customer review.")
![Immunofluorescence: Lightning-Link Fluorescein Antibody Labeling Kit [707-0010] - RAW264 macrophage cell line stained with chicken anti-F4/80 antibody conjugated to Fluorescein using Lightning-Link®, counter-stained with DAPI.](http://images.novusbio.com/fullsize/Lightning-Link-Fluorescein-Antibody-Labeling-Kit-Immunofluorescence-707-0010-img0002.jpg)
![Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Immunohistochemistry-Paraffin-NB100-77808-img0014.jpg "Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.")
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0012.jpg)
![Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal. IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Immunohistochemistry-NB100-77808-img0013.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0001.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0007.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor(R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa Fluor 700. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0008.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0009.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0011.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0010.jpg)