CEACAM8/CD66b Antibody (G10F5)

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody.

Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal. IHC image submitted by a verified customer review.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor(R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa Fluor 700. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Flow cytometry image submitted by a verified customer review.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verifed customer review.

Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.

• Reactivity: Hu, Mu
• Applications: Flow, IHC, IHC-Fr, IHC-P, CyTOF-ready, ICC/IF
• Clone: G10F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CEACAM8/CD66b Antibody (G10F5) Summary

• Immunogen: Human peripheral blood cells.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM8
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1ug/million cells
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: It is recommended that CD66b clone G10F5 be titrated for optimal performance for each application. This antibody is CyTOF ready. Use in Immunocytochemistry reported in scientific literature (PMID:33122293).

Reactivity Notes

Predicited to cross react with Chimpanzee. Use in Mouse reported in scientific literature (PMID:33122293).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: IgM purified

Alternate Names for CEACAM8/CD66b Antibody (G10F5)

• Carcinoembryonic antigen CGM6
• carcinoembryonic antigen gene family member 6
• carcinoembryonic antigen-related cell adhesion molecule 8
• CD66b antigen
• cd66b clone g10f5
• cd66b G10F5
• cd66b granulocyte marker
• cd66b ihc
• cd66b marker
• cd66b neutrophil marker
• cd66b neutrophils
• CD66b
• CD67 antigen
• CD67
• CEACAM8
• CEACAM-8
• CGM6
• NCA-95
• Non-specific cross-reacting antigen NCA-95

Background

CEACAM-8/CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM-8/CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM-8/CD66b expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c. Clone G10F5 can be used for a number of applications including Flow Cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEACAM8/CD66b Antibody (NB100-77808)(8)

Publications using NB100-77808

• Chen R, Cai Q, Lin P et al. Role of immune-inflamed phenotype in the prognosis of hypopharyngeal carcinoma following primary surgery Head & neck 2022-10-12 [PMID: 36222335]
• Rhoades JL Cibull ML Thompson JS et al. Role of natural killer cells in the pathogenesis of human acute graft-versus-host disease. Transplantation. [PMID: 8333033]
• Abdelnabi MN, Molina MF, Soucy G et al. Sex-Dependent Hepatoprotective Role of IL-22 Receptor Signaling in Non-Alcoholic Fatty Liver Disease-Related Fibrosis Cellular and molecular gastroenterology and hepatology 2022-08-13 [PMID: 35970323] (IHC-P, Human)
  
  Details:
  Human liver Tissue, dilution used 1:100
• Adachi A, Honda T, Egawa G et al. Estradiol suppresses psoriatic inflammation in mice by regulating neutrophil and macrophage functions The Journal of allergy and clinical immunology 2022-05-04 [PMID: 35589416] (IHC, Human)
• Pinzaru AM, Kareh M, Lamm N et al. Met Mutation is a Potential Therapeutic Target for Advanced Endometrial Cancer Genes Dev 2020-09-28 [PMID: 33122293] (ICC, IHC, Mouse)
• Au-Yeung CL, Yeung TL, Achreja A et al. ITLN1 modulates invasive potential and metabolic reprogramming of ovarian cancer cells in omental microenvironment Nat Commun 2020-07-15 [PMID: 32669559] (IHC-P, Human)
• Giurisato E, Lonardi S, Telfer B et al. Extracellular-regulated protein kinase 5-mediated control of p21 expression promotes macrophage proliferation associated with tumor growth and metastasis Cancer Res. 2020-06-19 [PMID: 32561530] (IHC, Human)

Reviews for CEACAM8/CD66b Antibody (NB100-77808) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 07/28/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Spleen
• Species: Human
• Lot: A-2

Comments

• Comments: IHC-P was performed using a Leica bond autostainer.
  Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS

reviewed by: Verified Customer IHC Human 06/09/2017

Summary

• Application: IHC
• Sample Tested: Liver cancer tissue
• Species: Human

Comments

• Comments: IHC - Single staining of CEACAM-8/CD66b on FFPE section of human liver. Primary Ab is mouse IgM kappa monoclonal Ab from Novus Biologicals (NB100-77808). Secondary Ab is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. The cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal.

Bioinformatics

• Gene Symbol: CEACAM8
• Entrez:
  • 1088(Human)
• Uniprot:
  • P31997()