VE-Cadherin Antibody (123413) [Unconjugated]

Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VE-Cadherin was detected in immersion fixed HUVEC cells using Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immortalized HAECs retain phenotypic and functional characteristics of primary cells. (A) (Top) Immortalized HAEC confluent monolayers stained with CD31-AF488 antibody (green) and DAPI (blue). (Bottom) Flow cytometry histogram plots of primary or immortalized HAECs labeled with CD31-AF488 antibody. Secondary antibody only (dashed line) and nonstained cells (dotted line) were used as negative controls. (B) (Top) Immortalized HAEC confluent monolayers stained with VE-cadherin-AF488 (green) antibody, Acti-Stain 555 phalloidin (red), and DAPI (blue). (Bottom) Flow cytometry histogram plots of immortalized HAECs labeled with VE-cadherin-AF488 and ZO-1-AF647 antibodies. Secondary antibody only (dashed line) was used as a negative control. (C) Tube forming assay of primary (top) and immortalized (bottom) HAECs at 0 h and 8 h after seeding in Matrigel-coated wells. (D) Acetylated low-density lipoprotein (Ac-LDL) uptake assay of primary HAECs (top), immortalized HAECs (middle), and fibroblasts (negative control; bottom) treated with fluorescently labeled Ac-LDL (red) for 2.5 h and stained with DAPI (blue). (E) IL-8 detection in culture supernatants 24 h after treatment of primary or immortalized HAECs with increasing concentrations of TNF-alpha , IFN-gamma , or IL-6. (F) IL-8 detection in culture supernatants 24 h after treatment of immortalized HAECs with increasing concentrations of IL-2 or IL-1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29229737), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 123413
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

VE-Cadherin Antibody (123413) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
  Asp48-Gln593
  Accession # P33151
• Specificity: Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin and
  no cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 0.5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VE-Cadherin Antibody (123413) [Unconjugated]

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74% homology.

1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
3. Overduin, M. et al. (1995) Science 267:386.
4. Takeichi, M. (1991) Science 251:1451.
5. Nose, A. et al. (1987) EMBO J. 6:3655.
6. Carmeliet, P. et al. (1999) Cell 98:147.
7. Gory-Faure, S. et al. (1999) Development 126:2093.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VE-Cadherin Antibody (MAB9381)(29)

Publications using MAB9381

• Liu, C;Shen, M;Liu, Y;Manhas, A;Zhao, SR;Zhang, M;Belbachir, N;Ren, L;Zhang, JZ;Caudal, A;Nishiga, M;Thomas, D;Zhang, A;Yang, H;Zhou, Y;Ameen, M;Sayed, N;Rhee, JW;Qi, LS;Wu, JC; CRISPRi/a screens in human iPSC-cardiomyocytes identify glycolytic activation as a druggable target for doxorubicin-induced cardiotoxicity Cell stem cell 2024-11-07 [PMID: 39515331] (Immunocytochemistry, Human)
• Lietuvninkas, L;Baccouche, B;Kazlauskas, A; The Multi-Kinase Inhibitor RepSox Enforces Barrier Function in the Face of Both VEGF and Cytokines Biomedicines 2023-08-31 [PMID: 37760872] (ICC, Human)
• Eulashini Chuntharpursat-Bon, Oleksandr V. Povstyan, Melanie J. Ludlow, David J. Carrier, Marjolaine Debant, Jian Shi et al. PIEZO1 and PECAM1 interact at cell-cell junctions and partner in endothelial force sensing Communications Biology 2023-04-01 [PMID: 37005489]
• S Summer, E Rossmanith, M Pasztorek, C Fiedler, M Gröger, S Rauscher, V Weber, MB Fischer Mesenchymal stem cells support human vascular endothelial cells to form vascular sprouts in human platelet lysate-based matrices PLoS ONE, 2022-12-15;17(12):e0278895. 2022-12-15 [PMID: 36520838] (ICC, Human)
• Somasundaram Raghavan, Masuma Akter Brishti, Daniel Mohr Collier, M. Dennis Leo Hypoxia induces purinergic receptor signaling to disrupt endothelial barrier function Frontiers in Physiology 2022-11-21 [PMID: 36479340]
• XY Cui, B Stavik, B Thiede, PM Sandset, SM Kanse FSAP Protects against Histone-Mediated Increase in Endothelial Permeability In Vitro International Journal of Molecular Sciences, 2022-11-08;23(22):. 2022-11-08 [PMID: 36430180] (ICC, Human)
• Bowen Zhao, Yueqi Ni, Hong Zhang, Yin Zhao, Lu Li Endothelial deletion of TBK1 contributes to BRB dysfunction via CXCR4 phosphorylation suppression Cell Death Discovery 2022-10-28 [PMID: 36307391]
• Olmsted ZT, Paluh JL. A combined human gastruloid model of cardiogenesis and neurogenesis iScience 2022-06-17 [PMID: 35721464]
• Deepti Rana, Ajoy Kandar, Nasim Salehi-Nik, Ilyas Inci, Bart Koopman, Jeroen Rouwkema Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues Bioactive Materials 2022-06-01 [PMID: 35087964]
• Hao Chen, Huaxiao Yang, Chen Zhang, Si Chen, Xin Zhao, Mark Zhu et al. Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a Molecular NIR-II Cell Tracker Research (Wash D C) 2021-06-19 [PMID: 34250496]
• Kevin B. Denis, Jolene I. Cabe, Brooke E. Danielsson, Katie V. Tieu, Carl R. Mayer, Daniel E. Conway The LINC complex is required for endothelial cell adhesion and adaptation to shear stress and cyclic stretch Molecular Biology of the Cell 2021-08-19 [PMID: 34191529]
• Nicholas E. Propson, Ethan R. Roy, Alexandra Litvinchuk, Jörg Köhl, Hui Zheng Endothelial C3a receptor mediates vascular inflammation and blood-brain barrier permeability during aging Journal of Clinical Investigation 2021-01-04 [PMID: 32990682]
• R Daum, D Visser, C Wild, L Kutuzova, M Schneider, G Lorenz, M Weiss, S Hinderer, UA Stock, M Seifert, K Schenke-La Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture Cells, 2020-03-23;9(3):. 2020-03-23 [PMID: 32210018] (ICC, Human)
• Gesa L. Tiemeier, Rozemarijn de Koning, Gangqi Wang, Sarantos Kostidis, Rosalie G. J. Rietjens, Wendy M. P. J. Sol et al. Lowering the increased intracellular pH of human-induced pluripotent stem cell-derived endothelial cells induces formation of mature Weibel-Palade bodies Stem Cells Translational Medicine 2020-07-01 [PMID: 32163224]
• Marilina Antonelou, Erik Michaëlsson, Rhys D.R. Evans, Chun Jing Wang, Scott R. Henderson, Lucy S.K. Walker et al. Therapeutic Myeloperoxidase Inhibition Attenuates Neutrophil Activation, ANCA-Mediated Endothelial Damage, and Crescentic GN Journal of the American Society of Nephrology 2020-02-01 [PMID: 31879336]
• Antonina Akk, Luke E. Springer, Lihua Yang, Samantha Hamilton-Burdess, John D. Lambris, Huimin Yan et al. Complement activation on neutrophils initiates endothelial adhesion and extravasation Molecular Immunology 2019-10-01 [PMID: 31542608] (Western Blot, Immunocytochemistry, Human)
• Y Kiyan, S Tkachuk, K Kurselis, N Shushakova, K Stahl, D Dawodu, R Kiyan, B Chichkov, H Haller Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signalling Sci Rep, 2019-09-19;9(1):13591. 2019-09-19 [PMID: 31537875] (ICC, Human)
• Tácia Tavares Aquinas Liguori, Gabriel Romero Liguori, Luiz Felipe Pinho Moreira, Martin Conrad Harmsen Adipose tissue–derived stromal cells’ conditioned medium modulates endothelial‐mesenchymal transition induced by IL‐1 beta /TGF‐ beta 2 but does not restore endothelial function Cell Proliferation 2019-11-01 [PMID: 31468648]
• T Neri, E Hiriart, PP van Vliet, E Faure, RA Norris, B Farhat, B Jagla, J Lefrancois, Y Sugi, T Moore-Morr, S Zaffran, RS Faustino, AC Zambon, JP Desvignes, D Salgado, RA Levine, JL de la Pomp, A Terzic, SM Evans, R Markwald, M Pucéat Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis Nat Commun, 2019-04-26;10(1):1929. 2019-04-26 [PMID: 31028265] (ICC, Human)
• Jarel K. Gandhi, Travis Knudsen, Matthew Hill, Bhaskar Roy, Lori Bachman, Cynthia Pfannkoch-Andrews et al. Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions Stem Cells Translational Medicine 2019-06-01 [PMID: 30768863] (Immunocytochemistry, Human)
• BL Ecker, A Kaur, SM Douglass, MR Webster, FV Almeida, GE Marino, AJ Sinnamon, MG Neuwirth, GM Alicea, A Ndoye, M Fane, X Xu, MS Sim, GB Deutsch, MB Faries, GC Karakousis, AT Weeraratna Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis Cancer Discov, 2018-10-02;9(1):82-95. 2018-10-02 [PMID: 30279172] (ICC, Human)
• J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 2018-06-13;0(0):9636897187803. 2018-06-13 [PMID: 29895168] (Flow Cytometry, Human)
• K Kulhankova, KJ Kinney, JM Stach, FA Gourronc, IM Grumbach, AJ Klingelhut, W Salgado-Pa The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function Infect. Immun., 2018-02-20;0(0):. 2018-02-20 [PMID: 29229737] (Flow Cytometry, ICC, Human)
• JC Bramley, CG Drummond, NJ Lennemann, CA Good, KS Kim, CB Coyne A Three-Dimensional Cell Culture System To Model RNA Virus Infections at the Blood-Brain Barrier mSphere, 2017-06-21;2(3):. 2017-06-21 [PMID: 28656176] (ICC, Human)
• Gabriela Odent Grig Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells J Tissue Eng Regen Med, 2016-12-12;0(0):. 2016-12-12 [PMID: 27943613] (Flow Cytometry, Human)
• Paola Orecchia, Romana Conte, Enrica Balza, Gabriella Pietra, Maria Cristina Mingari, Barbara Carnemolla Targeting Syndecan-1, a molecule implicated in the process of vasculogenic mimicry, enhances the therapeutic efficacy of the L19-IL2 immunocytokine in human melanoma xenografts Oncotarget 2015-10-09 [PMID: 26460958]
• Anusuya Das, Steven M. Lenz, Anthony O. Awojoodu, Edward A. Botchwey Abluminal Stimulation of Sphingosine 1-Phosphate Receptors 1 and 3 Promotes and Stabilizes Endothelial Sprout Formation Tissue Engineering Part A 2015-01-01 [PMID: 25315888]
• Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs. Cell Stem Cell, 2008-07-03;3(1):55-68. 2008-07-03 [PMID: 18593559] (ICC, Mouse)
• Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption. Am. J. Physiol. Heart Circ. Physiol., 2007-03-23;293(1):H610-9. 2007-03-23 [PMID: 17384130] (ICC, Human)

Bioinformatics

• Gene Symbol: CDH5
• Uniprot:
  • P33151()