S1P1/EDG-1 Antibody (713412) [Allophycocyanin]

HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and eGFP was stained with either (A) Rat Anti-Mouse S1P1/EDG-1 APC-conjugated Monoclonal Antibody (Catalog # FAB7089A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 APC-conjugated Monoclonal Antibody (Catalog # FAB7089A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 713412
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

S1P1/EDG-1 Antibody (713412) [Allophycocyanin] Summary

• Immunogen: Mouse S1P₁/EDG‑1 synthetic peptide (T4-H28)
  Accession # O08530
• Specificity: Detects mouse S1P₁/EDG‑1 peptide in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: S1PR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P1/EDG-1 Antibody (713412) [Allophycocyanin]

• CD363 antigen
• CD363
• CHEDG1
• D1S3362
• EDG1
• EDG-1
• EDG1edg-1
• Endothelial differentiation G-protein coupled receptor 1
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 1
• FLJ58121
• S1P receptor 1
• S1P receptor Edg-1
• S1P1
• S1P1ECGF1
• S1PR1
• sphingosine 1-phosphate receptor 1
• sphingosine 1-phosphate receptor EDG1
• Sphingosine 1-phosphate receptor Edg-1
• sphingosine-1-phosphate receptor 1

Background

S1P₁ (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P₁ is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P₁ share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S1P1/EDG-1 Antibody (FAB7089A)(7)

Publications using FAB7089A

• Rashu, R;Ninkov, M;Wardell, CM;Benoit, JM;Wang, NI;Meilleur, CE;D'Agostino, MR;Zhang, A;Feng, E;Saeedian, N;Bell, GI;Vahedi, F;Hess, DA;Barr, SD;Troyer, RM;Kang, CY;Ashkar, AA;Miller, MS;Haeryfar, SMM; Targeting the MR1-MAIT cell axis improves vaccine efficacy and affords protection against viral pathogens PLoS pathogens 2023-06-29 [PMID: 37384813] (Flow Cytometry, Mouse)
• Xiong, W;Chen, S;Xiang, H;Zhao, S;Xiao, J;Li, J;Liu, Y;Shu, Z;Ouyang, J;Zhang, J;Liu, H;Wang, X;Zou, H;Chen, Y;Chen, A;Lu, H; S1PR1 attenuates pulmonary fibrosis by inhibiting EndMT and improving endothelial barrier function Pulmonary pharmacology & therapeutics 2023-06-07 [PMID: 37295666] (Flow Cytometry, Mouse)
• P Chongsathi, C Jackson, S Koyama, F Loebel, X Cui, SH Farber, K Woroniecka, AA Elsamadicy, CA Dechant, HR Kemeny, L Sanchez-Pe, TA Cheema, NC Souders, JE Herndon, JV Coumans, JI Everitt, BV Nahed, JH Sampson, MD Gunn, RL Martuza, G Dranoff, WT Curry, PE Fecci Sequestration of T cells in bone marrow in the setting of glioblastoma and other intracranial tumors Nat. Med., 2018-08-13;0(0):. 2018-08-13 [PMID: 30104766] (Flow Cytometry, Mouse)
• R Mashud, A Nomachi, A Hayakawa, K Kubouchi, S Danno, T Hirata, K Matsuo, T Nakayama, R Satoh, R Sugiura, M Abe, K Sakimura, S Wakana, H Ohsaki, S Kamoshida, H Mukai Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1 Sci Rep, 2017-08-09;7(1):7663. 2017-08-09 [PMID: 28794483] (Flow Cytometry, Mouse)
• IL4RA on lymphatic endothelial cells promotes T cell egress during sclerodermatous graft versus host disease JCI Insight, 2016-08-04;1(12):. 2016-08-04 [PMID: 27547823] (Flow Cytometry, Mouse)
• Cuenca M, Romero X, Sintes J, Terhorst C, Engel P Targeting of Ly9 (CD229) Disrupts Marginal Zone and B1 B Cell Homeostasis and Antibody Responses. J Immunol, 2015-12-14;196(2):726-37. 2015-12-14 [PMID: 26667173] (Flow Cytometry, Mouse)
• Othy S, Hegde P, Topcu S, Sharma M, Maddur M, Lacroix-Desmazes S, Bayry J, Kaveri S Intravenous gammaglobulin inhibits encephalitogenic potential of pathogenic T cells and interferes with their trafficking to the central nervous system, implicating sphingosine-1 phosphate receptor 1-mammalian target of rapamycin axis. J Immunol, 2013-03-22;190(9):4535-41. 2013-03-22 [PMID: 23526819] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: S1PR1
• Uniprot:
  • O08530()